Inactivation of various bacteriophages by different ultraviolet technologies: Development of a reliable virus indicator system for water reuse

Bae, Kyung Seon; Shin, Gwy-Am

doi:10.4491/eer.2016.032

Cited by 13 publications

(6 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, phages with circular single-stranded DNA are more sensitive than those with linear single-stranded RNA. Those with linear double-stranded DNA exhibited the highest photoreactivation following UV exposure [81,82].…”

Section: Discussionmentioning

confidence: 99%

Bacteriophage as a potential therapy to control antibiotic-resistant Pseudomonas aeruginosa infection through topical application onto a full-thickness wound in a rat model

Rezk

Abdelsattar

Elzoghby

et al. 2022

Journal of Genetic Engineering and Biotechnology

View full text Add to dashboard Cite

Background Antibiotic-resistant Pseudomonas aeruginosa (P. aeruginosa) is one of the most critical pathogens in wound infections, causing high mortality and morbidity in severe cases. However, bacteriophage therapy is a potential alternative to antibiotics against P. aeruginosa. Therefore, this study aimed to isolate a novel phage targeting P. aeruginosa and examine its efficacy in vitro and in vivo. Results The morphometric and genomic analyses revealed that ZCPA1 belongs to the Siphoviridae family and could infect 58% of the tested antibiotic-resistant P. aeruginosa clinical isolates. The phage ZCPA1 exhibited thermal stability at 37 °C, and then, it decreased gradually at 50 °C and 60 °C. At the same time, it dropped significantly at 70 °C, and the phage was undetectable at 80 °C. Moreover, the phage ZCPA1 exhibited no significant titer reduction at a wide range of pH values (4–10) with maximum activity at pH 7. In addition, it was stable for 45 min under UV light with one log reduction after 1 h. Also, it displayed significant lytic activity and biofilm elimination against P. aeruginosa by inhibiting bacterial growth in vitro in a dose-dependent pattern with a complete reduction of the bacterial growth at a multiplicity of infection (MOI) of 100. In addition, P. aeruginosa-infected wounds treated with phages displayed 100% wound closure with a high quality of regenerated skin compared to the untreated and gentamicin-treated groups due to the complete elimination of bacterial infection. Conclusion The phage ZCPA1 exhibited high lytic activity against MDR P. aeruginosa planktonic and biofilms. In addition, phage ZCPA1 showed complete wound healing in the rat model. Hence, this research demonstrates the potential of phage therapy as a promising alternative in treating MDR P. aeruginosa.

show abstract

Section: Discussionmentioning

confidence: 99%

Bacteriophage as a potential therapy to control antibiotic-resistant Pseudomonas aeruginosa infection through topical application onto a full-thickness wound in a rat model

Rezk

Abdelsattar

Elzoghby

et al. 2022

Journal of Genetic Engineering and Biotechnology

View full text Add to dashboard Cite

show abstract

“…Briefly, for each bacteriophage, the top agar layer having a confluent lysis of host cells was harvested by scraping into a small volume of phosphate-buffered saline (PBS), and bacteriophages were extracted with an equal volume of chloroform. The supernatant was recovered by low speed (4,000 x g) centrifugation for 30 min at 4°C and stored at −80°C until use [24].…”

Section: Preparation Of Virus Samplementioning

confidence: 99%

Sensing viruses using terahertz nano-gap metamaterials

Park

Shin

et al. 2017

Biomed. Opt. Express

161

117

View full text Add to dashboard Cite

We demonstrate highly sensitive detection of viruses using terahertz split-ring resonators with various capacitive gap widths. Two types of viruses, with sizes ranging from 60 nm (PRD1) to 30 nm (MS2), were detected at low densities on the metamaterial surface. The dielectric constants of the virus layers in the THz frequency range were first measured using thick films, and the large values found identified them as efficient target substances for dielectric sensing. We observed the resonance-frequency shift of the THz metamaterial following deposition of the viruses on the surface at low-density. The resonance shift was higher for the MS2 virus, which has a relatively large dielectric constant. The frequency shift increases with surface density until saturation and the sensitivity is then obtained from the initial slope. Significantly, the sensitivity increases by about 13 times as the gap width in the metamaterials is decreased from 3 µm to 200 nm. This results from a combination of size-related factors, leading to field enhancement accompanying strong field localization.

show abstract

“…For systems treating oxidized, filtered wastewaters, Title 22 requires that processes provide a CT (product of total chlorine residual and contact time) value of 450 mg-min/L with a contact time of at least 90 min. Alternatively, systems must use spiking tests to demonstrate that the system can achieve 5-log inactivation of the virus surrogate, bacteriophage MS2 ( CDPH, 2014 ; Bae and Shin, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Control of sulfides and coliphage MS2 using hydrogen peroxide and UV disinfection for non-potable reuse of pilot-scale anaerobic membrane bioreactor effluent

et al. 2021

View full text Add to dashboard Cite

Highlights Anaerobic membrane bioreactor effluent was treated for non-potable reuse. Photon shielding by HS − inhibited UV inactivation of MS2 bacteriophage. Hydrogen peroxide oxidized sulfides to sulfate at pH ≥ 8. 180–225 mJ cm −2 UV doses achieved 5-log MS2 inactivation post-H 2 O 2 . Anaerobic reuse was competitive with aerobic reuse at <285 μM sulfides.

show abstract

Inactivation of various bacteriophages by different ultraviolet technologies: Development of a reliable virus indicator system for water reuse

Cited by 13 publications

References 10 publications

Bacteriophage as a potential therapy to control antibiotic-resistant Pseudomonas aeruginosa infection through topical application onto a full-thickness wound in a rat model

Bacteriophage as a potential therapy to control antibiotic-resistant Pseudomonas aeruginosa infection through topical application onto a full-thickness wound in a rat model

Sensing viruses using terahertz nano-gap metamaterials

Control of sulfides and coliphage MS2 using hydrogen peroxide and UV disinfection for non-potable reuse of pilot-scale anaerobic membrane bioreactor effluent

Contact Info

Product

Resources

About