Inapparent Persistent Virus Infection in Continuously Grown Aedes aegypti Mosquito Cells

Peleg, J.

doi:10.1099/0022-1317-5-4-463

Cited by 93 publications

(34 citation statements)

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“…aegypti; posteriormente, se establecieron otras líneas celulares de esta misma especie obtenidas, en orden cronológico, por Singh en 1967 (10), Peleg en 1968 (11,12) (14)(15)(16)(17).…”

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Características de nuevos cultivos celulares derivados de tejidos embrionarios de Aedes aegypti (Diptera: Culicidae).

Silva¹,

Escovar²,

Bello³

2005

biomedica

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Introducción. Los cultivos celulares de insectos son una metodología útil en estudios biomédicos y tecnológicos. Objetivo. El propósito principal del presente trabajo fue obtener y caracterizar cultivos celulares derivados de tejidos embrionarios de Aedes aegypti. Materiales y métodos. Se emplearon huevos embrionados para los explantes de tejidos en los medios de cultivos MM/VP12 y L-15/Grace, con suplemento de 20% de suero fetal bovino y una mezcla al 1% de antibiótico y antimicótico, con un rango de pH entre 6,8 y 7,0. Los cultivos se incubaron a una temperatura de 28 o C sin atmósfera de CO 2 . Resultados. El crecimiento celular se obtuvo en el medio L-15/Grace, 3 semanas después de haber sido sembrados los tejidos embrionarios; sin embargo, se necesitaron 6 meses para la formación de la monocapa confluente. Desde agosto de 2003 hasta junio de 2004, se habían realizado 28 subcultivos. Las células se caracterizaron morfológicamente; predominaron las formas epitelioides en subcultivos de pases altos. También se reconocieron las particularidades morfométricas del cariotipo y, además, se determinaron los perfiles isoenzimáticos y moleculares de los cultivos celulares, los cuales se compararon con muestras de adultos de la especie tomadas de la misma colonia y con líneas celulares derivadas de otros insectos. Discusión. Estas células representan, potencialmente, un importante sistema in vitro en investigaciones básicas y aplicadas. Palabras clave: Aedes aegypti, cultivos celulares, vesículas, cariotipos, isoenzimas, RAPD-PCR.Characteristics of new cell cultures derived from embryonic tissues of Aedes aegypti (Diptera: Culicidae) Introduction. Cell cultures from insects are a useful methodology in technological and biomedical studies.Objective. The present work was aimed at obtaining and characterizing cell cultures derived from Aedes aegypti embryonic tissues. Materials and methods. Embryonated eggs were used for embryonic tissue explants in L-15/ Grace and MM/VP12 culture media, supplemented with 20% fetal bovine serum and a mixture of 1% antimycotic and antibiotics, at a pH ranging from 6.8 to 7.0. The incubation temperature was 28 o C; a CO 2 atmosphere was not required. Results. Cell growth was obtained in L-15/Grace medium three weeks after embryonic tissues explants. Six months were required for achieving a confluent monolayer. Twenty-eight serial cell subcultures were carried out from August 2003 to June 2004. Cell morphology was characterized as epithelial in the later subcultures. Karyotype morphometry as well as molecular and isozymatic profiles were established. The cultures were compared with adult samples from the species taken from the same colony and with cell lines derived from other insects.

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“…aegypti; posteriormente, se establecieron otras líneas celulares de esta misma especie obtenidas, en orden cronológico, por Singh en 1967 (10), Peleg en 1968 (11,12) (14)(15)(16)(17).…”

unclassified

Características de nuevos cultivos celulares derivados de tejidos embrionarios de Aedes aegypti (Diptera: Culicidae).

Silva¹,

Escovar²,

Bello³

2005

biomedica

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“…ever in the vertebrate cell infection results in cell death whereas in the mosquito cell the rate of cell division is unaltered and a persistent infection results (2,4,5).…”

mentioning

confidence: 99%

The growth of Semliki Forest virus in cultured mosquito cells: Ultrastructural observations

Raghow

Davey

Dalgarno

1973

Archiv f Virusforschung

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Togaviruses (8) replicate in both their vertebrate and invertebrate hosts. Recent studies in these two systems show that the initial growth kinetics of two representative Mphaviruses (8), Semliki Forest virus (1, 2) and Ross River virus (5) are similar in cultured vertebrate and invertebrate (Aedes albopictus) cells. However in the vertebrate cell infection results in cell death whereas in the mosquito cell the rate of cell division is unaltered and a persistent infection results (2, 4, 5).

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“…This relatively short period is in marked contrast to the 50-week interval reported by Igarashi (1979) for resistance to occur in dengue-1 PI cultures, indicating that we have not observed the same phenomenon. The possibility that the resistance is interferon-mediated is also unlikely, since the induction of resistance was not sensitive to actinomycin D. In addition, other investigators have shown that mosquito cells do not express interferon activity (Peleg, 1969;Murray & Morahan, 1973;Stollar & Shenk, 1973;Davey & Delgarno, 1974;Kascsak & Lyons, 1974).…”

Section: Simultaneous Infection Ofc6/36 Cells With Dengue-1 and Dengue-3mentioning

confidence: 99%

Demonstration of Interference between Dengue Virus Types in Cultured Mosquito Cells using Monoclonal Antibody Probes

Dittmar

Castro

Haines

1982

Journal of General Virology

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SUMMARYCultured Aedes albopictus cells (clone C6/36), persistently infected (PI) with dengue virus type 1 (dengue-1) were found resistant to superinfection with dengue virus type 3 (dengue-3). This was determined by indirect immunofluorescent (IF) staining of cultures using monoclonal antibody against a dengue-3 type-specific antigen. Dengue-1 PI cultures stained with this antibody 3 days after superinfection with dengue-3 virus (m.o.i. of 2) had dengue-3 antigen in 0.1 to 1.0% of the cells. Control cultures infected with dengue-3 at the same multiplicity contained denguek3 antigen in greater than 90 % of the cells. The resistance to superinfection was not interferon-mediated, and occurred within 20 h after primary infection. In cultures simultaneously infected with two dengue virus types, one virus type was excluded from replication in most cells. A small population of cells was also found (about 1%) that contained type-specific antigen of both dengue virus types.

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Inapparent Persistent Virus Infection in Continuously Grown Aedes aegypti Mosquito Cells

Cited by 93 publications

References 1 publication

Características de nuevos cultivos celulares derivados de tejidos embrionarios de Aedes aegypti (Diptera: Culicidae).

Características de nuevos cultivos celulares derivados de tejidos embrionarios de Aedes aegypti (Diptera: Culicidae).

The growth of Semliki Forest virus in cultured mosquito cells: Ultrastructural observations

Demonstration of Interference between Dengue Virus Types in Cultured Mosquito Cells using Monoclonal Antibody Probes

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