IncHI2 Plasmids Are the Key Vectors Responsible for
            <i>oqxAB</i>
            Transmission among Salmonella Species

Wong, Marcus Ho Yin; Chan, Edward Wai-Chi; Xie, Liqi; Li, Ruichao; Chen, Sheng

doi:10.1128/aac.01555-16

Cited by 35 publications

(29 citation statements)

References 18 publications

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“…The aac (6′)-Ib-cr gene is usually found in a cassette as part of an integron in a multi-resistance plasmid, which may contain other PMQR genes [34,35], and IS26 is often associated [36]. In pRJ749, aac (6′)-Ib-cr was located at a cluster of resistance genes flanked by IS26, which consisted of the aac (6′)-Ib-cr, bla OXA-1 , catB3, arr-3, qacEΔ1, and Δsul1 elements as previously reported [37]. This cassette structure is widely present in Enterobacteriaceae in China, as demonstrated by BLAST.…”

Section: Discussionmentioning

confidence: 59%

Emerging coexistence of three PMQR genes on a multiple resistance plasmid with a new surrounding genetic structure of qnrS2 in E. coli in China

Ying

Zhou

Xie

et al. 2020

Antimicrob Resist Infect Control

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Background: Quinolones are commonly used for treatment of infections by bacteria of the Enterobacteriaceae family. However, the rising resistance to quinolones worldwide poses a major clinical and public health risk. This study aimed to characterise a novel multiple resistance plasmid carrying three plasmid-mediated quinolone resistance genes in Escherichia coli clinical stain RJ749. Methods: MICs of ceftriaxone, cefepime, ceftazidime, ciprofloxacin, and levofloxacin for RJ749 and transconjugant c749 were determined by the Etest method. Conjugation was performed using sodium azide-resistant E. coli J53 strain as a recipient. The quinolone resistance-determining regions of gyrA, gyrB, parC, and parE were PCR-amplified. Results: RJ749 was highly resistant to quinolones, while c749 showed low-level resistance. S1-nuclease pulsed-field gel electrophoresis revealed that RJ749 and c749 both harboured a plasmid. PCR presented chromosomal mutation sites of the quinolone resistance-determining region, which mediated quinolone resistance. The c749 genome comprised a single plasmid, pRJ749, with a multiple resistance region, including three plasmid-mediated quinolone resistance (PMQR) genes (aac (6′)-Ib-cr, qnrS2, and oqxAB) and ten acquired resistance genes. One of the genes, qnrS2, was shown for the first time to be flanked by two IS26s. Three IS26-mediated circular molecules carrying the PMQR genes were detected. Conclusions:We revealed the coexistence of three PMQR genes on a multiple resistance plasmid and a new surrounding genetic structure of qnrS2 flanked by IS26 elements. IS26 plays an important role in horizontal spread of quinolone resistance.

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Section: Discussionmentioning

confidence: 59%

Emerging coexistence of three PMQR genes on a multiple resistance plasmid with a new surrounding genetic structure of qnrS2 in E. coli in China

Ying

Zhou

Xie

et al. 2020

Antimicrob Resist Infect Control

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“…Rapid spread of mcr‐1 is in part (if not all) due to its presence in different types of transmissible plasmids, which include IncI2, IncX4, IncP, IncHI1, IncHI2, IncF, IncFI, and IncFII 22. Of these, IncHI2 is the most common type in Salmonella 21,23 and the most active in acquiring foreign resistance genes 24,25. However, previous studies did not provide a comprehensive view and the evolution routes of the IncHI2 class of mcr‐1 ‐carrying plasmids.…”

Section: Introductionmentioning

confidence: 99%

“…[22] Of these, IncHI2 is the most common type in Salmonella [21,23] and the most active in acquiring foreign resistance genes. [24,25] However, previous studies did not provide a comprehensive view and the evolution routes of the IncHI2 class of mcr-1-carrying plasmids.…”

Section: Introductionmentioning

confidence: 99%

Carriage of Distinct mcr‐1‐Harboring Plasmids by Unusual Serotypes of Salmonella

et al. 2020

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Colistin acts as a last‐resort antibiotic against lethal infections by carbapenem‐resistant Enterobacterial pathogens. Enterobacteriaceae carrying mobile colistin resistance (MCR) genes are rapidly emerging and threatening human health and food safety. Despite mcr‐1 being prevalent in Escherichia coli, its dissemination in Salmonella is not well characterized. Herein, two unusual serotypes of colistin‐resistant Salmonella isolates are reported first, namely serotype Ngor (ST5399) and Goldcoast (ST2529). Using whole genome sequencing, it is shown that mcr‐1 is located on the IncHI2‐like plasmid pTB501 (188,527 bp) of strain SSDFZ54 and the IncX4‐type plasmid pTB602 (33,303 bp) in strain SSDFZ69, respectively. Furthermore, the backbone, tra‐ and antimicrobial resistance genes relative variable regions in the mcr‐1‐bearing IncHI2 plasmids are systematically characterized. Phylogenetic analysis shows that all IncHI2‐type plasmids from non‐Chinese regions are clustered together, suggesting a possible Chinese origin. Taken together, these findings extend the understanding of Salmonella as a vehicle of mcr‐1 carriage and distribution.

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“…Typhimurium in chicken from China and pHK0653 from clinical S . Typhimurium in Hong Kong (Wong et al, 2016 ), except that a 4,284-bp fragment ( floR -IS CR2 ) was absent in pHK0653 (Figures 2 , 3 ), further suggesting that the same or similar oqxAB -carrying IncHI2 plasmids are transmitted among Enterobacteriaceae isolates from various sources in different geographic regions of China. Additionally, the backbones of pHNLDF400 and pHNYJC8 showed 99% identity to those of our previously sequenced colistin-resistant mcr-1 -bearing plasmids pHNSHP45-2 (KU341381) obtained from porcine E. coli isolate SHP45 in Shanghai, China (Zhi et al, 2016 ) and pHNAH67 (KX246266) obtained from an E. coli isolate from chicken in Anhui province, China.…”

Section: Resultsmentioning

confidence: 92%

“…Indiana among animals in China, as well as in clinical S . Typhimurium isolates in Hong Kong (Li et al, 2013 ; Wong et al, 2016 ). Thus, this study was aimed at determining the distribution and genetic elements (IS 26 and plasmids) of oqxAB among E. coli isolates from different sources (animals, animal-derived food products, and human clinics) in Guangzhou, China, determining the complete nucleotide sequence of two IncHI2 plasmids carrying oqxAB and comparing them with those previously reported from different sources in China, and outlining the possible routes of oqxAB transmission via the food chain.…”

Section: Introductionmentioning

confidence: 99%

Characterization of oqxAB in Escherichia coli Isolates from Animals, Retail Meat, and Human Patients in Guangzhou, China

et al. 2017

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The purpose of this study was to investigate the prevalence and genetic elements of oqxAB among Escherichia coli isolates from animals, retail meat, and humans (patients with infection or colonization) in Guangzhou, China. A total of 1,354 E. coli isolates were screened for oqxAB by PCR. Fifty oqxAB-positive isolates were further characterized by pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), S1-PFGE, genetic environment analysis, plasmid replicon typing, and plasmid sequencing. oqxAB was detected in 172 (33.79%), 60 (17.34%), and 90 (18.07%) E. coli isolates from animal, food, and human, respectively. High clonal diversity was observed among oqxAB-positive isolates. In 21 oqxAB-containing transformants, oqxAB was flanked by two IS26 elements in the same orientation, formed a composite transposon Tn6010 in 19 transformants, and was located on plasmids (33.3~500 kb) belonging to IncN1-F33:A-:B- (n = 3), IncHI2/ST3 (n = 3), F-:A18:B- (n = 2), F-:A-:B54 (n = 2), or others. Additionally, oqxAB was co-located with multiple resistance genes on the same plasmid, such as aac(6′)-Ib-cr and/or qnrS, which were identified in two F-:A18:B- plasmids from pigs, and blaCTX−M−55, rmtB, fosA3, and floR, which were detected in two N1-F33:A-:B- plasmids from patients. The two IncHI2/ST3 oqxAB-bearing plasmids, pHNLDF400 and pHNYJC8, which were isolated from human patient and chicken meat, respectively, contained a typical IncHI2-type backbone, and were similar to each other with 2-bp difference, and also showed 99% identity to the Salmonella Typhimurium oqxAB-carrying plasmids pHXY0908 (chicken) and pHK0653 (human patient). Horizontal transfer mediated by mobile elements may be the primary mechanism underlying oqxAB spread in E. coli isolates obtained from various sources in Guangzhou, China. The transmission of identical oqxAB-carrying IncHI2 plasmids between food products and humans might pose a serious threat to public health.

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IncHI2 Plasmids Are the Key Vectors Responsible for oqxAB Transmission among Salmonella Species

Cited by 35 publications

References 18 publications

Emerging coexistence of three PMQR genes on a multiple resistance plasmid with a new surrounding genetic structure of qnrS2 in E. coli in China

Emerging coexistence of three PMQR genes on a multiple resistance plasmid with a new surrounding genetic structure of qnrS2 in E. coli in China

Carriage of Distinct mcr‐1‐Harboring Plasmids by Unusual Serotypes of Salmonella

Characterization of oqxAB in Escherichia coli Isolates from Animals, Retail Meat, and Human Patients in Guangzhou, China

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