Incidence and distribution of leek yellow stripe virus in allium crops in Serbia

Abstract

Leek yellow stripe virus (LYSV) is one of the most frequent and important viruses in leek and garlic crops worldwide. In Serbia this virus is found both in leek and garlic, and often at high percentages. During two consecutive years, 2012 and 2013, a total 92 samples were collected from 11 inspected leek-, garlic-and onion-growing locations and they were analyzed for the presence of LYSV using DAS-ELISA. LYSV was detected in 31.5% of the tested samples. In 2012, the presence of LYSV was only detected in leek plants, and in 55.6% of the tested samples. During 2013, LYSV was detected in 85% of leek and 58.3% of garlic samples. In total, LYSV was detected in 56.4% of leek samples and 17.1% garlic samples. LYSV incidence was confirmed using RT-PCR with LYSV specific primers amplifying 1020 bp fragment representing coat protein and part of nuclear inclusion B genes. Molecular identification was confirmed by sequencing of two selected isolates, 181-13 (MG242625) from garlic and 298-13 (MG242624) from leek, and comparing them to the GenBank sequences of LYSV. Phylogenetic analysis of 55 sequences of LYSV from all over the world showed some correlation between host plant and geographical origin of the isolates, forming five separate clades. Two Serbian LYSV isolates fell into distant clades. The Serbian leek isolate 298-13 of LYSV belongs to clade B, while isolate 181-13 originating from garlic belongs in clade E.

Serological and molecular detection of Leek yellow stripe virus infecting onion (Allium cepa L.) and leek (Allium ampeloprasum L.) in Ankara, Turkey

“…Therefore, the low LYSV infection rate could most likely be attributed to the absence of aphid vectors on fields that limit the spread of LYSV. Low LYSV infection rates on onions were also reported on surveys in other regions of Turkey and other countries (Dovas and Vovlas 2003, Fidan 2010, Sevik and Akcura 2013, Vučurović et al 2017). However, infection rate of 60.1% was observed in Tehran region of Iran (Shahraeen et al 2008).…”

Leek Yellow Stripe Virus Can Adjust for Host Adaptation by Trimming the N-Terminal Domain to Allow the P1 Protein to Function as an RNA Silencing Suppressor

“…LYSV infects garlic and leek and is detected in garlicgrowing areas worldwide. Although onions generally are not considered to be natural hosts for LYSV (Katis et al, 2012;Vučurović et al, 2017), onion damage due to LYSV infection has also been reported in some regions (Santosa and Ertunç, 2020;Sharhraeen et al, 2008). LYSV detected in garlic-growing areas in Japan can be classified into two types, northern type (LYSV-N) and southern type (LYSV-S), based on a characteristic deletion of 200-300 bases in the P1 gene (Takaki et al, 2005;Yoshida et al, 2012).…”

First Report of Leek Yellow Stripe Virus on Allium Sativum L. In Ukraine

“…Leek yellow stripe virus (LYSV) is one of the potyviruses infecting garlic in many countries including southern/eastern EU states (Vončina et al, 2016;Vučurović et al, 2017), but was never reported from Ukraine. Using doubleantibody sandwich enzyme-linked immunosorbent assay with virus-specific antibodies, LYSV was detected in 28% of samples from 3/4 screened regions of Ukraine, similarly to previously described OYDV in Ukraine (Snihur et al, 2019).…”