Incidence and genetic diversity of Peach latent mosaic viroid and Hop stunt viroid in stone fruits in Serbia

Abstract

Tissue-imprint hybridization (TIH) assay was validated for large-scale detection of Peach latent mosaic viroid (PLMVd) and Hop stunt viroid (HSVd). All 72 collected leaves (100%) from 2 PLMVd-and 2 HSVd-infected trees were positive in TIH, regardless of the geographic orientation of the scaffold, level of the canopy and position of the leaf in the shoot. In a large-scale survey in Serbia, we tested by TIH 871 trees of stone fruits, representing 602 cultivars from fruit collections in Belgrade, Čačak and Novi Sad. PLMVd was detected in 185 (50%) peach trees or 95 (54%) cultivars and HSVd in 2 apricot trees and cultivars (2%). The occurrence of HSVd is a new report for Serbia. No viroid infection was found in European plums, sweet cherries, sour cherries and wild Prunus spp. PLMVd-infected peach cultivars originated from the world's main breeding centres of this crop.Western European and Asian cultivars were the most infected (58%) followed by those originating from North America (50%). Nine PLMVd and two HSVd isolates were sequenced and analyzed. All showed PMLVd sequences clustered together in the previously reported phylogenetic group III. Both HSVd isolates were found to be derived from recombinant events, but that of the cv. Saturn represented a putative new phylogenetic group of HSVd.

Simultaneous detection and genetic variability of stone fruit viroids in the Czech Republic

“…However, HSVd was detected in three out of a total of 220 apricot trees, plus one accession of peach rootstocks, B-VA-2 (Table 2). Generally, the incidence of HSVd in the tested samples seemed to be low and consistent with results reported previously (Mandic et al 2008).…”

Simultaneous detection of six RNA plant viruses affecting tomato crops using a single digoxigenin-labelled polyprobe

“…Besides improving sensitivity, non-isotopic molecular hybridisation diagnostic methods have been concerned with reducing the processing time and the overall cost. In this way, in addition to the dot-blot, the tissue-printing technique has proved to be a simple and reliable method for sample preparation to detect plant viruses (Más and Pallas 1995;Cañizares et al 2001) and viroids (Romero-Durbán et al 1995;Mandic et al 2007).…”

Low incidence of peach latent mosaic viroid in peach mother blocks in Serbia

“…Afterwards, in a more comprehensive study, Mandić et al (2008) reported high PLMVd incidence (50%) in collection orchards. The viroid incidence in collections may be attributed to an initial introduction of contaminated plants.…”

“…In later studies, PLMVd was detected in more than half of the analyzed peach varieties from stone fruit collections (Mandić et al, 2008). PLMVd is a pathogen covered by special supervision of stone fruit mother blocks as regulated by the Ministry of Agriculture and Environmental Protection of the Republic of Serbia.…”