Incidence and Molecular Characterization of Carbapenemase Genes in Association with Multidrug-Resistant Clinical Isolates of Pseudomonas aeruginosa from Tertiary Healthcare Facilities in Southwest Nigeria

Olaniran, Oluwatoyin B; Adeleke, O E; Donia, Ahmed; Shahid, Ramla; Bokhari, Habib

doi:10.1007/s00284-021-02706-3

Cited by 10 publications

(9 citation statements)

References 42 publications

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“…A study conducted at a tertiary healthcare facility in southwest Nigeria also found a prevalence of 38.4%. These values are in agreement with those of our study and within the values that were verified [ 44 ]. A recent study conducted in Korea revealed that bla NDM-1 was among the most detected metallo-beta-lactamase genes in carbapenem-resistant P. aeruginosa isolates.…”

Section: Discussionsupporting

confidence: 94%

Antimicrobial Resistance, Genetic Lineages, and Biofilm Formation in Pseudomonas aeruginosa Isolated from Human Infections: An Emerging One Health Concern

Silva,

López

et al. 2023

Antibiotics

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Pseudomonas aeruginosa (PA) is a leading nosocomial pathogen and has great versatility due to a complex interplay between antimicrobial resistance and virulence factors. PA has also turned into one the most relevant model organisms for the study of biofilm-associated infections. The objective of the study focused on analyzing the antimicrobial susceptibility, resistance genes, virulence factors, and biofilm formation ability of thirty-two isolates of PA. PA isolates were characterized by the following analyses: susceptibility to 12 antimicrobial agents, the presence of resistance genes and virulence factors in PCR assays, and the quantification of biofilm production as evaluated by two distinct assays. Selected PA isolates were analyzed through multilocus sequence typing (MLST). Thirty PA isolates have a multi-resistant phenotype, and most of the isolates showed high levels of resistance to the tested antibiotics. Carbapenems showed the highest prevalence of resistance. Various virulence factors were detected and, for the quantification of biofilm production, the effectiveness of different methods was assessed. The microtiter plate method showed the highest accuracy and reproducibility for detecting biofilm-producing bacteria. MLST revealed four distinct sequence types (STs) in clinical PA, with three of them considered high-risk clones of PA, namely ST175, ST235, and ST244. These clones are associated with multidrug resistance and are prevalent in hospitals worldwide. Overall, the study highlights the high prevalence of antibiotic resistance, the presence of carbapenemase genes, the diversity of virulence factors, and the importance of biofilm formation in PA clinical isolates. Understanding these factors is crucial for effective infection control measures and the development of targeted treatment strategies.

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Section: Discussionsupporting

confidence: 94%

Antimicrobial Resistance, Genetic Lineages, and Biofilm Formation in Pseudomonas aeruginosa Isolated from Human Infections: An Emerging One Health Concern

Silva,

López

et al. 2023

Antibiotics

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“…While the majority (7/16; 44%) of the isolates carried bla-VIM, only 19% (3/16) carried bla-NDM-1. The current investigation shows a much higher prevalence of MBL genes among P. aeruginosa isolates compared to similar studies conducted in India (12%), Pakistan (18%), China (55%), Iran (40%), and Nigeria (17%) (25)(26)(27)(28). Moreover, it needs to be noted that we investigated the presence of only four variants (bla-IMP, bla-VIM, bla-SPM, and bla-NDM-1).…”

Section: Discussioncontrasting

confidence: 46%

Metallo-β-lactamase mediated rapid increase in carbapenem resistance ofPseudomonas aeruginosa

Anjum,

Mitu,

Arefin

et al. 2024

Preprint

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Antibiotic-resistantPseudomonas aeruginosais a common nosocomial pathogen all over the world. We detected the presence ofP. aeruginosain 22% (53 out of 238) of the test samples collected from patients with infections including secondary wound infections, abscesses and urinary tract infections admitted to two academic hospitals in Bangladesh. Resistance to carbapenems (imipenem, and meropenem) was present among 30% (16 out of 53) of these clinical P. aeruginosa isolates, which is more than 2-fold higher compared to that of previous studies. Such a rapid increase in carbapenem resistance was mediated by metallo-β-lactamase (MBL). Expression of MBL was detected in 90% (14 out of 16) of these resistant isolates. Molecular analyses revealed that the carbapenem-resistant isolates carried at least one of the MBL variants, either bla-VIM or bla-NDM-1. All the bla-NDM-1 positives carried a 0.5 MDa plasmid. ERIC-PCR revealed the highly heterogeneous nature of theP. aeruginosaisolates indicating multiple sources of infection within the hospital. However, the majority of XDR isolates belonged to a single cluster of drug-resistant bacterial infections. These findings indicate that Metallo-β-lactamase (MBL) mediated resistance to carbapenem inP. aeruginosaposes a serious threat to the spread of infections among hospitalized patients.

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“…23 A recent study from Southwest Nigeria found that 17% of clinical P. aeruginosa isolates were extensively drug resistant and harboured metallo-β-lactamase genes. 28 Thirteen different carbapenemase and ESBL genes were detected in a collection of 430 isolates, while in the present study four different carbapenemase and two different ESBL genes were identified in 123 isolates. A recent study from Northeast Nigeria reported bla NDM-1 in one clinical P. aeruginosa isolate of ST-773, in addition to other carbapenemase-encoding genes ( bla NDM , bla KPC ) found in other Gram-negative clinical isolates.…”

Section: Discussionsupporting

confidence: 41%

High incidence of carbapenemase-producingPseudomonas aeruginosaclinical isolates from Lagos, Nigeria

Olalekan

Bader

Iwalokun

et al. 2023

JAC-Antimicrobial Resistance

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Background Carbapenem-resistant Pseudomonas aeruginosa strains are on the rise worldwide. This study characterized clinical isolates of P. aeruginosa from three Nigerian hospitals for carbapenem resistance. Methods Strains isolated from wounds (n = 88), urine/catheter tips (n = 25), sputum/tracheotomy aspirates (n = 5), ear swabs (n = 4) and vaginal swabs (n = 1) were identified by MALDI-TOF and antibiotic susceptibility testing was performed using the VITEK 2 system. The genomic DNA of each isolate was subject to sequencing using Illumina and Oxford nanopore technology. Bioinformatics analyses were performed to detect antimicrobial resistance genes, clonal affiliations and phylogenetic relations of 123 non-duplicate P. aeruginosa isolates, whereas assembly of the nanopore reads using the plasmIDent pipeline enabled the identification of plasmids. Results Forty-three percent of the isolates were resistant to all antibiotic categories tested. More than 40% of the isolates were resistant to the carbapenems imipenem and/or meropenem (39% and 44%, respectively). Among the meropenem-resistant isolates, 48 (89%) carried at least one carbapenemase gene. The predominant one was blaNDM-1 (n = 34), which conferred resistance to all five antibiotic categories and highly increased the MICs of both meropenem and imipenem. The other recurrent carbapenemase genes were blaVIM-2 (n = 4), and blaVIM-5-like (n = 11), which co-existed with blaNDM-1 in two isolates. Conclusions The study revealed a high rate of carbapenem resistance and conjugative, broad host range plasmids carrying carbapenemase-encoding genes, especially the NDM-1 type, among isolates of P. aeruginosa. This may forebode the emergency of ubiquitous carbapenem resistance urging the implementation of infection control and antimicrobial stewardship strategies in Nigerian hospitals.

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Incidence and Molecular Characterization of Carbapenemase Genes in Association with Multidrug-Resistant Clinical Isolates of Pseudomonas aeruginosa from Tertiary Healthcare Facilities in Southwest Nigeria

Cited by 10 publications

References 42 publications

Antimicrobial Resistance, Genetic Lineages, and Biofilm Formation in Pseudomonas aeruginosa Isolated from Human Infections: An Emerging One Health Concern

Antimicrobial Resistance, Genetic Lineages, and Biofilm Formation in Pseudomonas aeruginosa Isolated from Human Infections: An Emerging One Health Concern

Metallo-β-lactamase mediated rapid increase in carbapenem resistance ofPseudomonas aeruginosa

High incidence of carbapenemase-producingPseudomonas aeruginosaclinical isolates from Lagos, Nigeria

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