Incidence and Polymerase Chain Reaction Assay of Listeria monocytogenes from Raw Milk in Gyeongnam Province of Korea

Ha, Kwang-Soo; Park, Seon-Ja; Seo, Sook Jae; Park, Jung Hyun; Chung, Duck-Hwa

doi:10.4315/0362-028x-65.1.111

Cited by 10 publications

(4 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Our results are lower than those of other studies carried out in other countries such as Turkey and Korea, where the prevalence was 4% for the first city (Vardar-Ünlü et al, and 2% for the second (Ha et al, 2002). In Europe, the prevalence of listeriosis increased by 19.1% between 200819.1% between and 200919.1% between (EFSA, 2011.…”

Section: Discussioncontrasting

confidence: 73%

Prevalence of Listeria monocytogenes in Raw Cow's Milk in the Region of Batna, Algeria

Sabrina¹,

Mamache²,

Hamdi³

et al. 2021

American Journal of Animal and Veterinary Sciences

View full text Add to dashboard Cite

The aim of this study is the detection of the presence of L. monocytogenes in raw milk in the region of Batna using the real-time polymerase chain reaction (real-time PCR) technique, in order to assess the riskrun by the consumer of raw milk in the study area. 65 milk samples were investigated for the detection of L. monocytogenes. The detection of the hlyA gene in all samples was done by real-time PCR assay by comparative cycle threshold method. The results obtained were negative for all the samples analyzed, therefore the prevalence of L. monocytogenes in the 65 samples collected in this study is 0%. The absence of L. monocytogenes in the samples could be linked to the good health of the selected cattle. In fact, L. monocytogenes is considered to be very widespread in industrialized countries and rare in North Africa and Algeria.

show abstract

Section: Discussioncontrasting

confidence: 73%

Prevalence of Listeria monocytogenes in Raw Cow's Milk in the Region of Batna, Algeria

Sabrina¹,

Mamache²,

Hamdi³

et al. 2021

American Journal of Animal and Veterinary Sciences

View full text Add to dashboard Cite

show abstract

“…En Algérie la prévalence de L. monocytogenes varie de 1,9% à 3,2% (Bellouni, 1990;Lebres & Mouffok, 2000;Hamdi et al, 2007). Dans d'autres pays comme la Turquie et la Corée, la prévalence était 6% (Vardar-Ünlü et al, 1998), 2% pour le second (Ha et al, 2002;Jemmi & Stephan, 2006). En Europe, la prévalence de listériose a augmenté 19% entre 2008(EFSA, 2011.…”

Section: Résultats Et Discussionunclassified

“…Des études effectuées en Italie et en Suisse ont montré des résultats négatifs (Ha et al, 2002;Jemmi & Stephan, 2006). D'autre part, une étude réalisée au Liban sur cinquante échantillons de fromage Akkawi choisis au hasard dans la région Liban Nord et analysés par la technique Mini-VIDAS, a montré que parmi les cinquante échantillons, cinq étaient positifs (Dabboussi et al, 2012).…”

Section: Résultats Et Discussionunclassified

Activités biologiques des extraits aqueux de Pergularia tomentosa L. (Asclepiadaceae)

Kassaa¹,

Omari²,

Saati³

et al. 2016

LSJ

View full text Add to dashboard Cite

Al Kassaa Imad, Khaled el Omari, Marwa Saati, Bachar Ismail et Monzer Hamze. 2016. Prévalence de Listeria monocytogenes dans le lait cru de vache au Liban Nord. Lebanese Science Journal, 17(1): 37-43. La listériose, bien qu'elle soit une zoonose, est une pathologie invasive qui peut affecter les nouveau-nés, les femmes enceintes ainsi les adultes immunodéprimés. Les manifestations cliniques se traduisent par des gastroentérites fébriles, des formes sévères invasives incluant les septicémies, méningites, rhombencéphalites, infections prénatales et avortements. Les espèces de Listeria sont des bactéries ubiquitaires, capables de s'adapter à l'environnement, chez l'animal et les aliments végétaux. Dans ce travail, notre objectif a été d'évaluer la présence de Listeria monocytogenes dans du lait cru collecté au Liban Nord. Au total, cent échantillons de lait de vache ont été analysés par la technique Grand VIDAS (Biomérieux France). Les résultats obtenus ont révélé l'absence de Listeria monocytogenes dans tous les échantillons analysés. ABSTRACT Al Kassaa Imad, Khaled el Omari, Marwa Saati, Bachar Ismail and Monzer Hamze. 2016. Prevalence of Listeria monocytogenes in raw cow milk in north Lebanon. Lebanese Science Journal, 17(1): 37-43. Listeriosis, although a zoonosis, is an invasive disease that can affect newborns, pregnant women and immunocompromised adults. Clinical manifestations can be expressed by febrile gastroenteritis, invasive forms including severe sepsis, meningitis, rhombencephalitis, prenatal infections and abortions. Species of Listeria bacteria are ubiquitous and adaptable to the environment in animal and plant foods. This study aimed to determine the prevalence of Listeria monocytogenes in 100 samples of fresh cow milk

show abstract

“…Another adaptation of PCR that also uses multiple primer sets is nested PCR; however, these primer sets are used in sequential reactions and are designed against the same target. It is used to increase sensitivity and specificity and has been used for the identification of L. monocytogenes in clinical samples [57,[107][108][109][110], environmental samples [78], and milk samples [97,111,112]. Commercially available PCR tests are the BAXÒ PCR system (Qualicon, Wilmington, DE, USA) and the ProbeliaÒ assay (Sanofi Diagnostic Pasteur, Marne la Coquette, France), which have been trialed in the field on a variety of different sample types [113][114][115][116][117][118][119][120][121][122].…”

Section: Polymerase Chain Reaction (Pcr)mentioning

confidence: 99%

Methods for the isolation and identification ofListeriaspp. andListeria monocytogenes: a review

Hughes²,

2005

View full text Add to dashboard Cite

Listeria monocytogenes is an important food-borne pathogen and is widely tested for in food, environmental and clinical samples. Identification traditionally involved culture methods based on selective enrichment and plating followed by the characterization of Listeria spp. based on colony morphology, sugar fermentation and haemolytic properties. These methods are the gold standard; but they are lengthy and may not be suitable for testing of foods with short shelf lives. As a result more rapid tests were developed based on antibodies (ELISA) or molecular techniques (PCR or DNA hybridization). While these tests possess equal sensitivity, they are rapid and allow testing to be completed within 48 h. More recently, molecular methods were developed that target RNA rather than DNA, such as RT-PCR, real time PCR or nucleic acid based sequence amplification (NASBA). These tests not only provide a measure of cell viability but they can also be used for quantitative analysis. In addition, a variety of tests are available for sub-species characterization, which are particularly useful in epidemiological investigations. Early typing methods differentiated isolates based on phenotypic markers, such as multilocus enzyme electrophoresis, phage typing and serotyping. These phenotypic typing methods are being replaced by molecular tests, which reflect genetic relationships between isolates and are more accurate. These new methods are currently mainly used in research but their considerable potential for routine testing in the future cannot be overlooked.

show abstract

Incidence and Polymerase Chain Reaction Assay of Listeria monocytogenes from Raw Milk in Gyeongnam Province of Korea

Cited by 10 publications

References 17 publications

Prevalence of Listeria monocytogenes in Raw Cow's Milk in the Region of Batna, Algeria

Prevalence of Listeria monocytogenes in Raw Cow's Milk in the Region of Batna, Algeria

Activités biologiques des extraits aqueux de Pergularia tomentosa L. (Asclepiadaceae)

Methods for the isolation and identification ofListeriaspp. andListeria monocytogenes: a review

Contact Info

Product

Resources

About