Incidence and Sero-survey of Canine Adenovirus Type 2 in Various Animal Species

Yang, Dong-Kun; Kim, Ha-Hyun; Yoon, Soon-Seek; Ji, Miryun; Cho, In‐Soo

doi:10.4167/jbv.2018.48.3.102

Cited by 8 publications

(16 citation statements)

References 21 publications

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“…CAVs can infect several animal species, including pet dogs, and wild animals such as wolves, coyotes, skunks, foxes, bears, and lions (3,4). CAV infection of unvaccinated wild raccoon dogs was confirmed by serological testing (5,6). CAV-1 and CAV-2 give rise to infectious canine hepatitis and infectious laryngotracheitis, respectively, due to their propensity to infect different cell types (7,8).…”

Section: Introductionmentioning

confidence: 94%

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Recharacterization of the Canine Adenovirus Type 1 Vaccine Strain based on the Biological and Molecular Properties

et al. 2019

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Section: Introductionmentioning

confidence: 94%

“…Diverse biological techniques, including immunofluorescence (IF) assays, are used to diagnose CAV infections in dogs (4,10). VN, hemagglutination inhibition (HI), and indirect IF assays also provide information on the biological features of CAVs (6,11).…”

Section: Introductionmentioning

confidence: 99%

Recharacterization of the Canine Adenovirus Type 1 Vaccine Strain based on the Biological and Molecular Properties

et al. 2019

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“…Dulbecco's modified Eagle's medium (DMEM) (Gibco BRL, USA) containing 5% (v/v) fetal bovine serum (FBS; Gibco BRL, USA), penicillin (100 IU/mL), streptomycin (100 µg/ mL), and amphotericin B (0.25 µg/mL) was used to maintain Madin-Darby canine kidney (MDCK) cells (CCL34; ATCC, USA) at 37°C in 5% (v/v) CO 2 incubator. The APQA1701-40P strain of CAV-2, which had undergone 40 serial passages in MDCK cell culture, was used as the antigen for I-ELISA and the VN test [7]. Bloods from 165 dogs residing in Chungbuk and Gyeongbuk provinces between 2016 and 2018 were collected and their sera were subjected to the VN and I-ELISA tests.…”

Section: Cells Virus and Serum Samplesmentioning

confidence: 99%

“…There are several methods for detecting canine adenovirus (CAV) antigen, including virus isolation, polymerase chain reaction, histopathology, rapid immunochromatographic strip assay and immunohistochemistry [6][7][8][9]. CAV antibodies induced by inoculation of vaccine or natural infection can also be measured by several methods, such as hemagglutination inhibition (HI), virus neutralization (VN), enzyme-linked immunosorbent assay (ELISA), and the indirect immunofluorescence assay in animal serum samples [10,11].…”

Section: Introductionmentioning

confidence: 99%

“…Although VN test is accurate and useful for detecting CAV-2 antibodies, the method takes 4 to 5 days and is not suitable for large-scale sero-surveillance [11]. Previously, we reported a sero-survey of CAV-2 conducted with the VN test in various animal species [7]. However, ELISA is the preferred method for detecting CAV-2 antibodies in large-scale investigations because it does not require live CAV-2 and only a small amount of serum is needed; the procedure is also relatively simpler and safer than that of the VN test.…”

Section: Introductionmentioning

confidence: 99%

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Development of indirect ELISA for the detection of canine adenovirus type 2 antibodies in dog sera

et al. 2020

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Background: Canine adenovirus type 2 (CAV-2) induces infectious laryngotracheitis in members of the family Canidae, including dogs. To date, no ELISA kits specific for CAV-2 antibody have been commercialized for dogs in Korea. Objectives: We aimed to develop new indirect enzyme-linked immunosorbent assay (I-ELISA) to perform rapid, accurate serological surveys of CAV-2 in dog serum samples. Methods: In total, 165 serum samples were collected from dogs residing in Chungbuk and Gyeongbuk provinces between 2016 and 2018. The Korean CAV-2, named the APQA1701-40P strain, was propagated in Madin-Darby canine kidney cells and purified in an anionexchange chromatography column for use as an antigen for I-ELISA. The virus-neutralizing antibody titers of CAV-2 in the dog sera were measured by virus neutralization (VN) test. Results: We compared the results obtained between the VN and new I-ELISA tests. The sensitivity, specificity, and accuracy of new I-ELISA were 98.6%, 86.4% and 97.0% compared with VN test, respectively. New I-ELISA was significantly correlated with VN (r = 0.91). Conclusions: These results indicate that new I-ELISA is useful for sero-surveillance of CAV-2 in dog serum.

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Keratouveitis in juvenile dogs and its presumed association with canine adenovirus infection

Joyce

Oliver

Fricker³

et al. 2023

Veterinary Ophthalmology

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Objective We hypothesized that keratouveitis still occurs despite current widespread use of Canine adenovirus (CAV)‐2 vaccinations and assessed the utility of CAV‐1 and CAV‐2 titers in elucidation of its etiopathogenesis. Animals studied Nine dogs with unexplained keratouveitis (14 eyes) and nine control dogs. Procedures The Animal Health Trust clinical database was searched between 2008 and 2018 to identify cases of keratouveitis. Inclusion criteria included known vaccination status, interval from vaccination to development of clinical signs and availability of CAV titers. Cases were excluded if they were older than 1 year of age, or other causative ocular pathology for corneal edema was identified. Nine age‐matched dogs without corneal edema but with CAV titers were included as controls. Results Mean CAV‐1 and CAV‐2 titers were not statistically different between dogs with keratouveitis and controls (p = .16 and p = .76, respectively). Three cases had CAV‐1 titers >5000 and two of these cases had rising convalescence titers (greater than an 11‐fold increase) suggesting infection with wild‐type CAV‐1. The six other cases did not appear to be associated with CAV infection or vaccination. Conclusion Keratouveitis continues to occur despite the advent of CAV‐2 vaccinations. While this study found no evidence to indicate CAV‐2 vaccination causes keratouveitis, the data indicates that in a proportion of cases, contemporaneous wild‐type CAV‐1 infection is a possible cause.

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Incidence and Sero-survey of Canine Adenovirus Type 2 in Various Animal Species

Cited by 8 publications

References 21 publications

Recharacterization of the Canine Adenovirus Type 1 Vaccine Strain based on the Biological and Molecular Properties

Recharacterization of the Canine Adenovirus Type 1 Vaccine Strain based on the Biological and Molecular Properties

Development of indirect ELISA for the detection of canine adenovirus type 2 antibodies in dog sera

Keratouveitis in juvenile dogs and its presumed association with canine adenovirus infection

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