2019
DOI: 10.1364/ol.44.001710
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Incidence angle calibration for prismless total internal reflection fluorescence microscopy

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Cited by 8 publications
(11 citation statements)
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References 26 publications
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“…We milled periodic nano-hole arrays into a thin Au-lm deposited on the same BK-7 glass substrate as used before. We observed on a dark-eld microscope structural colors that varied among plasmonic hole arrays according to their periodicities, P, i.e., inter-hole spacing (340, 380 and 420 nm) 10,20,22 Fig. 3c shows typical transmission spectra obtained from those arrays upon white-light illumination.…”
Section: Ultra-thin Polymer Layers Have Homogenous Ri Over Hundred µMmentioning
confidence: 99%
See 1 more Smart Citation
“…We milled periodic nano-hole arrays into a thin Au-lm deposited on the same BK-7 glass substrate as used before. We observed on a dark-eld microscope structural colors that varied among plasmonic hole arrays according to their periodicities, P, i.e., inter-hole spacing (340, 380 and 420 nm) 10,20,22 Fig. 3c shows typical transmission spectra obtained from those arrays upon white-light illumination.…”
Section: Ultra-thin Polymer Layers Have Homogenous Ri Over Hundred µMmentioning
confidence: 99%
“…We excited TPPS uorescence at 488 nm, using TIRF. The focused laser spot is seen twice in the periphery of the BFP image, marking the positions of the incoming and back-re ected beam, respectively, and allowing a direct calibration of the beam angle 𝜗 from the BFP image 22,23 . As expected from the evanescent-wave decay in axial direction, the detected intensity dropped with increasing Δ, Fig.…”
Section: Ultra-thin Polymer Layers Have Homogenous Ri Over Hundred µMmentioning
confidence: 99%
“…In one study, a thin homogenous layer of fluorophores in air was used to produce a SAF ring of known radius r c (air) ¼ f obj that was compared to the annular distribution of excitation light in azimuthal beam-spinning TIRF with the fluorescence filter removed (65). Two follow-up studies used a similar strategy but with either a thin layer of organic dye for TIRF-SIM calibration (45) or else with Qdots embedded in poly(methyl methacrylate) for A-TIRFM (66). In the latter case, the fluorophore layer was topped with solutions of different, known RI (previously measured with an Abbe refractometer), and the laser spot (seen when the emission filter was taken out) was steered to these known angles to attain a more precise multiangle calibration.…”
Section: Saf Applications With Aperture Filteringmentioning
confidence: 99%
“…Generally, researchers [8][9][10] simply map BFP image pixel positions to incident angles by using the maximum aperture angle as the benchmark, because it theoretically corresponds to the maximum radius of a BFP image. However, manufacturing and assembly errors of an objective lens, also with a size-extended imaging spot, usually lead to the above mapping relationship inaccurate [11]. For this problem, Lee et al [12] developed an incident angle calibration method by introducing a point source to search the aperture angle-dependent maximum pupil radius.…”
Section: Introductionmentioning
confidence: 99%