Incidence of Bacterial and Fungal Contamination of Donor Corneas Preserved by Organ Culture

Hagenah, M.; Böhnke, Matthias; Engelmann, Katrin; Winter, R.

doi:10.1097/00003226-199507000-00012

Cited by 51 publications

(42 citation statements)

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“…These results fall within the range expected from previously published data (Garweg et al 1989;Hagenah et al 1995;Frueh et al 1995). Due to a low overall incidence of contaminations we would have needed a 6-ford higher number of donor pairs to detect an anticipated difference in the contamination rate of 10%.…”

Section: Discussionsupporting

confidence: 93%

Corneoscleral discs excised from enucleated and non‐enucleated eyes are equally suitable for transplantation

Garweg

Hagenah

Engelmann

et al. 1997

Acta Ophthalmologica Scandinavica

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ABSTRACT.Purpose: To assess whether the biological quality of corneoscleral tissue dissected in situ is, after organ culture, comparable to that harvested after enucleation.Merhoh: Corneoscleral discs were prepared from 23 donor eyes, either after enucleation, under laminar flow conditions (right eyes; group 1) or by direct excision in situ (left eyes; group 2). Endothelial cell counts were made and the degree oftissue contamination assessed both prior to and upon termination of organ culture.

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Section: Discussionsupporting

confidence: 93%

Corneoscleral discs excised from enucleated and non‐enucleated eyes are equally suitable for transplantation

Garweg

Hagenah

Engelmann

et al. 1997

Acta Ophthalmologica Scandinavica

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“…In the present study, the mean death-to-tissue retrieval time (8.4 h) was quite short as compared with that in previous reports (10-26 h). 5,13,18,24,25 We did not find any relationships between death-topreservation time and the risk of contamination, whereas previous reports had suggested that a death-topreservation time of more than 15 h was associated with increased risk of positive microbial rim culture. 26 However, this result was significant only in univariate analysis and not in multivariate analysis.…”

Section: Discussioncontrasting

confidence: 87%

Influencing factors on the suitability of organ-cultured corneas

Gavrilov

Borderie

Laroche

et al. 2010

Eye

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“…[9][10][11][12][13][14][15] The Hamburg eye bank found 4.7% fungal and 2.6% bacterial contaminants in 4546 tested cultures. 9 In contrast, the Besançon group reported 1.1% fungal and 4.6% bacterial contamination in 1156 tested storage media.…”

Section: Discussionmentioning

confidence: 99%

Time Course of Antibiotic and Antifungal Concentrations in Corneal Organ Culture

Seiler

Tschopp

Zimmerli

et al. 2016

Cornea

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Purpose: Contamination with bacteria and/or fungi is a serious complication in organ-cultured corneas. Hence, antibiotic and antifungal agents are added to the culture medium. The concentration of different antimicrobial and antifungal additives to the media over time has so far not been investigated in detail and is the aim of this study.Methods: Nine human fresh corneoscleral discs were stored in corneal culture medium consisting of 2% fetal bovine serum and minimal essential medium. In addition, the culture medium contained 1200 mg/mL penicillin G, 25 mg/mL amphotericin B, 120 mg/mL streptomycin, and 100 mg/mL voriconazole. The concentration of amphotericin B used was 10 times higher than in clinical routine to facilitate its detection. The cultures were kept at 37°C for 28 days. At days 0, 7, 14, 21, and 28, samples of the culture medium were harvested for analysis of antimicrobial concentrations by liquid chromatography and electrospray ionization tandem mass spectrometry.Results: During corneal storage, the concentration of all antibiotics and antifungal agents declined significantly. By day 28, penicillin G was reduced to 14% of the original concentration. Amphotericin B and streptomycin retained approximately 60% of the original concentration to the end of the experiment and voriconazole maintained stable concentrations after an initial decline to approximately 80% at 7 days.Conclusions: Throughout the entire storage period, the concentrations of penicillin G, streptomycin, and voriconazole exceeded the minimum inhibitory concentrations of all common contaminants, obviating the need for a change of the medium for antimicrobial reasons. Based on the minimum inhibitory concentrations and our findings, the initial concentration of amphotericin B should be raised to 5 mg/mL. Warm preservation at 31 to 37°C is more commonly used in European eye banks: corneas can be stored up to 4 weeks in organ culture medium consisting of minimum essential medium with the addition of 2% fetal bovine serum.2-6 Antibiotics and antifungal agents are added to the organ culture media to kill contaminants and to avoid iatrogenic infections at transplantation. Despite this prophylaxis, severe complications like endophthalmitis can still occur. 7,8 Little is known about the decay characteristics and efficacy over time of antimicrobial agents kept at body temperature. Therefore, we studied the concentration of the most commonly used antimicrobial and antifungal additives over time under the standard conditions established at the local eye bank at the Department of Ophthalmology, University Hospital in Bern. METHODS Sample PreparationNine human corneoscleral discs unsuitable for transplantation were excised under sterile conditions from intact globes not later than 6 hours post mortem and stored each in a corneal culture medium consisting of 2% fetal bovine serum and minimal essential medium (Sigma Aldrich, St Louis, MO) following the standard protocol of the eye bank of the University Hospital of Bern. The culture medium contained 1...

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Incidence of Bacterial and Fungal Contamination of Donor Corneas Preserved by Organ Culture

Cited by 51 publications

References 0 publications

Corneoscleral discs excised from enucleated and non‐enucleated eyes are equally suitable for transplantation

Corneoscleral discs excised from enucleated and non‐enucleated eyes are equally suitable for transplantation

Influencing factors on the suitability of organ-cultured corneas

Time Course of Antibiotic and Antifungal Concentrations in Corneal Organ Culture

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