Incidence of bovine clinical mastitis in Jammu region and antibiogram of isolated pathogens

Bhat, Adil Majid; Soodan, J.S.; Singh, Ranjit; Dhobi, Ishfaq Ahmad; Hussain, Tufail; Dar, Mohammad Yousuf; Mir, Masood Saleem

doi:10.14202/vetworld.2017.984-989

Cited by 41 publications

(41 citation statements)

References 17 publications

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“…The overall prevalence of CM was found to be 12.0% (30/250). Similar figures have been reported for CM among dairy cattle in India (11.5%) [ 7 ] and Ethiopia (12.5%) [ 60 ], although its prevalence among dairy cattle in China is estimated to be lower (3.3%) [ 19 ]. The frequency of bacterial species isolated from the CM cases indicated the occurrence of environmental ( E .…”

Section: Discussionsupporting

confidence: 76%

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Prevalence and etiology of mastitis in dairy cattle in El Oro Province, Ecuador

Amer

Gálvez

Fukuda

et al. 2018

The Journal of Veterinary Medical Science

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This study described the occurrence of clinical and subclinical forms of mastitis in 250 cattle from 5 dairy farms around the cities of Santa Rosa and Machala, El Oro Province, Ecuador. Clinical mastitis (CM) was determined based on obvious changes in milk (mild), signs of inflammation in the udder (moderate), and/or generalized clinical symptoms (severe). Subclinical mastitis (SCM) was assessed using the California mastitis test. CM and SCM were detected in 30 (12.0%) and 150 (60%) of the 250 tested cattle, respectively. Prevalence at the udder quarter level was 57.7% (577/1,000), which was higher among forequarters (369/577; 63.9%) than hindquarters. Of the 577 mastitic milk samples subjected to microbiological analysis, 35 were excluded due to contamination and 20 tested negative. Identification of bacterial isolates revealed that 33.3% of the 93 CM samples contained coliforms, 25.8% coagulase-positive staphylococci, 20.4% coagulase-negative staphylococci (CNS), 9.7% streptococci, 7.5% Bacillus spp., and 3.2% Klebsiella spp. Bacterial profiling of the 429 SCM milk samples showed that 55.4% contained CNS, 22.1% Bacillus spp., 9.3% streptococci, and 6.1% coagulase-positive staphylococci. In vitro antibiotic susceptibility testing of the obtained isolates indicated that all were susceptible to amoxicillin, ampicillin, cefotaxime, enrofloxacin, sulfamethoxazole-trimethoprim, gentamicin, and neomycin. No multidrug-resistant strains were observed.

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Section: Discussionsupporting

confidence: 76%

“…However, it has been reported that Sta . aureus predominates in clinical cases in India and Ethiopia [ 7 , 50 ].…”

Section: Discussionmentioning

confidence: 99%

Prevalence and etiology of mastitis in dairy cattle in El Oro Province, Ecuador

Amer

Gálvez

Fukuda

et al. 2018

The Journal of Veterinary Medical Science

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“…Higher prevalence of mastitis in multiparous cows might be ascribed to loosening of sphincter and patency of teat canal in older cows. Moreover, the median ligaments, which provide support to the teat, also get relaxed with age leading to hanging of udder and thus it makes more prone to mastitis (Boujenane et al, 2015;Bhat et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Prevalence, risk factors and bacterial causes of bovine mastitis in southern Ethiopia

Abebe¹,

Abera²,

Denbarga³

et al. 2020

Ethiop. Vet. J.

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Mastitis is one of the most economically important diseases in dairy farms worldwide. It is particularly important in Ethiopia where no routine prevention and control practices are in place. This cross-sectional study was carried out between October 2017 and June 2018 to estimate the prevalence of mastitis, identify the associated risk factors and isolate bacterial causes in dairy farms located in southern Ethiopia using standard microbiological methods and questionnaire survey. A total of 686 lactating cows which were found in 122 selected dairy farms were investigated by physical examination and California mastitis test. The overall cow-level prevalence of mastitis was 54.2% (95% CI: 50.5 – 57.9%). Based on the study site, the prevalence was 55.7% in Hawassa, 54.3% in Arsi Negele, 52.6% each in Wondo Genet and Wolayta Soddo towns with no significant (p > 0.05) difference among the sites. The majority of mastitis cases were subclinical (48.1%) while the clinical mastitis was only 6.1%. Of the 122 herds tested, 109 (89.3%; 95% CI: 82.1 – 93.9%) had at least a cow positive for mastitis. The study showed that high parity number (OR = 1.6; p = 0.015), flat (OR = 4.5; p <0.001) and round (OR = 2; p <0.001) teat end shape, history of mastitis in preceding lactation (OR = 3.3; p <0.001), and slightly (OR = 3.5; p < 0.001), moderately (OR = 4.9; p < 0.001), and very dirty (OR = 9.2; p < 0.001) udder and legs were the major risk factors which are significantly associated with higher prevalence of mastitis. Based on the available media and reagents, the major bacteria isolated from subclinical mastitic milk samples were Staphylococcus spp. (57.3%), Streptococcus spp. (18.6%), E. coli (17.3%) and Bacillus spp. (7.5%) in order of their abundance. The present study revealed a high prevalence of mastitis, particularly the subclinical one, and the associated risk factors. Enhancing the awareness of dairy farmers, regular screening of cows for subclinical mastitis, proper treatment of the clinical cases, improving the hygienic condition of the cows, and culling of chronically infected cows are critically important to prevent and control bovine mastitis. Keywords: Mastitis, Prevalence, Risk factors, Southern Ethiopia

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“…Although the prevalence of clinical mastitis is lower than subclinical, it can result in severe milk loss and a high risk of cow culling. Escherichia coli are the etiological agents often isolated from clinical cases in India and China [6,7]. It is normally found in environment and gastrointestinal tract of humans and animals [8].…”

Section: Introductionmentioning

confidence: 99%

Antiseptic effect of natural teat dip containing lactic acid against mastitis-causing Escherichia coli

et al. 2019

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Aim: This study aimed to estimate the enumeration of total bacteria and coliform on teat skin from dairy cows and evaluate the efficacy of the natural rice gel containing 5% v/v lactic acid (NGL) against Escherichia coli standard and field strains isolated from bovine teat skin. Materials and Methods: A total of 100 bacterial teat skin samples (25 cows) were collected from dairy cows in smallholder farm. The cows were housed in freestall barns. The colonization of total bacteria and E. coli on teat skin was measured by 3M Petrifilm method. The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of lactic acid were evaluated for reference strain of E. coli ATCC 25922 and two field strains of E. coli. The natural teat sanitizer was formulated using 5% NGL with modified rice gel. In vitro antiseptic efficacy of 5% NGL was determined by time-kill kinetic assay. E. coli morphology after exposure with 5% NGL was examined under a scanning electron microscope (SEM). Results: The total bacteria and coliform counts from bovine teat skin were 2.11×104 and 1.54×101 colony-forming units/ml, respectively. The MIC and MBC of lactic acid on the tested bacteria were 0.5% v/v. The natural teat dip was successfully prepared with minimum change in consistency after 1 year of storage at 4°C. The reduction rate of 5% NGL on E. coli ATCC 25922 and field strain showed 32.77% and 27.58%, respectively. An appearance under SEM of non-viable E. coli after being incubated with 5% NGL clearly showed atypical form and rough surface cell membrane. Conclusion: The rice gel containing 5% v/v lactic acid is a promising preparation as a natural teat antiseptic for reducing bacteria on teat skin. It was shown to be effective against E. coli causing bovine mastitis in dairy cows.

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Incidence of bovine clinical mastitis in Jammu region and antibiogram of isolated pathogens

Cited by 41 publications

References 17 publications

Prevalence and etiology of mastitis in dairy cattle in El Oro Province, Ecuador

Prevalence and etiology of mastitis in dairy cattle in El Oro Province, Ecuador

Prevalence, risk factors and bacterial causes of bovine mastitis in southern Ethiopia

Antiseptic effect of natural teat dip containing lactic acid against mastitis-causing Escherichia coli

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