Incidence of class A extended-spectrum  -lactamases in Champagne-Ardenne (France): a 1 year prospective study

Brasme, Lucien; Nordmann, Patrice; Fidel, F.; Lartigue, M.-F.; Bajolet, Odile; Poirel, Laurent; Forte, Dominique; Vernet-Garnier, Véronique; Madoux, Janick; Reveil, J.C.; Alba-Sauviat, C.; Baudinat, I.; Bineau, P.; Bouquigny-Saison, C.; Eloy, C.; Lafaurie, C.; Siméon, D.; Verquin, J. P.; Noël, Fabrice; Strady, C.; Champs, Christophe de

doi:10.1093/jac/dkm319

Cited by 51 publications

(16 citation statements)

References 34 publications

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“…The presence of bla CTX-M-15 has previously been associated with E. coli strains of phylogroups B2 and D, and in some instances, with specifi c PFGE types (9)(10)(11)(12)16). We detected an emerging and globally disseminated CTX-M-15 phylogroup B2 E. coli strain corresponding to the ST131 that was responsible for clonal outbreaks in Canada, France, Spain, and Portugal (11,14,16,23). Other CTX-M-15 B2 strains belong to clonal complexes ST695, ST405, ST354, or ST28, which have previously been detected in different geographic areas among isolates that do not express CTX-M-15 (online Appendix Figure, available from www.cdc.gov/EID/content/14/2/195-appG.htm).…”

Section: Discussionmentioning

confidence: 79%

“…These strains and plasmids were considered representative of these areas because they either caused outbreaks or were the fi rst isolates recovered in those countries (3,11,16,19,(21)(22)(23). Samples were isolated from urine (n = 33/43, 77%), wounds (n = 4/43, 9.%), respiratory tract infections (n = 3/43, 7%) and other sites (1 from feces, 1 from an intravenous catheter, and 1 from blood) in hospitalized patients.…”

Section: Bacterial Strains Production Of Esbl and Susceptibility Tementioning

confidence: 99%

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Dissemination of Clonally RelatedEscherichia coliStrains Expressing Extended-Spectrum β-Lactamase CTX-M-15

Coque

Novais

Carattoli

et al. 2008

Emerg. Infect. Dis.

668

469

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Section: Discussionmentioning

confidence: 79%

Section: Bacterial Strains Production Of Esbl and Susceptibility Tementioning

confidence: 99%

Dissemination of Clonally RelatedEscherichia coliStrains Expressing Extended-Spectrum β-Lactamase CTX-M-15

Coque

Novais

Carattoli

et al. 2008

Emerg. Infect. Dis.

668

469

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“…Our study is original, because it involved a set of consecutive nonduplicated and nonselected strains and because 50% of all tested strains were chromosomal AmpC producers not covered by some ESBL detection guidelines (3,6). In addition, we compared numerous phenotypic methods, including some modified methods that have not been systematically evaluated.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Nine Phenotypic Methods for Detection of Extended-Spectrum β-Lactamase Production by Enterobacteriaceae

et al. 2011

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The detection of extended-spectrum ␤-lactamase-producing (ESBL) bacteria is of importance for infection control and epidemiological surveillance. We aimed to compare phenotypic methods available in the routine laboratory and to evaluate two-step strategies using these methods for the detection of ESBL-positive Enterobacteriaceae. Two methods used for routine susceptibility testing (Vitek2 and disk diffusion methods) and seven methods designed for the detection of ESBL production (ESBL Etests, combination disks, double-disk synergy [DDS] methods on Mueller-Hinton [MH] agar and cloxacillin-containing MH agar, and the Cica-Beta test) were tested against 107 strains of Enterobacteriaceae not susceptible to extended-spectrum cephalosporins. All strains were screened for the presence of acquired ESBL-encoding genes by PCR, and the PCR result was considered the gold standard for evaluation of the other test methods. Among the 107 strains, 52 (49%) were ESBL positive. With Vitek2, sensitivities were the highest when using extended cards (73% to 79%), but 25% to 31% of the strains yielded indeterminate results. For the disk diffusion method, sensitivities were the highest (96%) when testing at least cefotaxime, cefepime, and a third compound (ceftazidime, cefpodoxime, or aztreonam). For the specific methods, specificities ranged from 62% (ceftazidime ESBL Etest) to 100% (DDS using a disk spacing of 20 mm). When a method designed for ESBL detection was used on strains considered ESBL negative or with an indeterminate result by a first routine susceptibility method, sensitivities reached 100% for a majority of combinations. In conclusion, two-step strategies using phenotypic methods available in most clinical laboratories may reach a sensitivity of 100% for ESBL detection among a large panel of species, including AmpC producers, providing a sensible choice of tests.

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“…Different expert systems revealed only a penicillinase pattern, without any alert on expanded-spectrum cephalosporin MICs. This difficulty was previously observed with other TEMtype ESBL such as the TEM-24 or CMT type, especially TEM-125 (23)(24)(25). As with the clinical TEM-125-producing strain TO799, TEM-187 was not easy to detect following either the American (26,27).…”

mentioning

confidence: 88%

TEM-187, a New Extended-Spectrum β-Lactamase with Weak Activity in a Proteus mirabilis Clinical Strain

Corvec

Beyrouthy

Crémet

et al. 2013

Antimicrob Agents Chemother

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A Proteus mirabilis clinical strain (7001324) was isolated from urine sample of a patient hospitalized in a long-term-care facility. PCR and cloning experiments performed with this strain identified a novel TEM-type ␤-lactamase (TEM-187) differing by four amino acid substitutions (Leu21Phe, Arg164His, Ala184Val, and Thr265Met) from TEM-1. This characterization provides further evidence for the diversity of extended-spectrum ␤-lactamases (ESBL) produced by P. mirabilis and for their potential spread to other Enterobacteriaceae due to a lack of sensitive detection methods used in daily practice. Before the CTX-M era, TEM-type extended-spectrum ␤-lactamases (TEM-ESBL) were the most prevalent mechanism of resistance to ␤-lactam antibiotics in Enterobacteriaceae. They emerged from the parental penicillinases TEM-1 and TEM-2 (1). Since the 1990s, different enzymes that combine novel substitutions leading to an ESBL pattern in Proteus mirabilis isolates such as TEM-10, TEM-24, TEM-52, TEM-72, TEM-87, and TEM-92 have been reported (2-6).In 2002, during a study on ESBL detection and the agreement between four different techniques, Leverstein-van Hall et al. identified a novel TEM variant that associated the Arg164His substitution observed in numerous TEM-ESBL with Leu21Phe and Thr265Met substitutions (7). This ␤-lactamase, designated TEM-75, was produced by Escherichia coli or Klebsiella pneumoniae strains and is easily detected by different methods; the ESBL-Etest method was considered the best. Recently, we reported on an ESBL-producing Proteus mirabilis isolate incorrectly detected as a TEM-24-producing clone recovered from urine of spinal cord injury patients (8). During this outbreak period, one patient was previously infected by a new TEM-derived ESBL called TEM-187 with a new combination of four substitutions in bla TEM gene.In this study, we characterized the genetic support and the enzymatic activity of TEM-187. A Proteus mirabilis clinical strain (7001324) was isolated from a urine sample of a patient hospitalized in the Physical Medicine Department at Nantes University Hospital, France. This patient had been treated with different antibiotics for urinary tract colonization/infections in the previous months. P. mirabilis 7001324 harbored a high level of resistance to amoxicillin and ticarcillin but was fully susceptible to penicillinclavulanate combinations and expanded-spectrum cephalosporins according to the results determined with a Vitek2 automated system with an AST-N103 card (bioMérieux, Marcy l'Etoile, France) or with a Phoenix automated system with an NMIC-93 gallery (BD Diagnostics, Sparks, MD), using a standard protocol. The double-disk synergy test (Mast Cica-␤ ESBL test) was negative for P. mirabilis 7001324 (9). Alone, a modified double-disk test with a 35-mm interdisk distance between ceftazidime-and amoxicillin-clavulanate-containing disks was positive. ␤-Lactam MICs were determined by a microdilution method on MuellerHinton agar (BD) with an inoculum of 10 4 CFU per spot (Table 1). P. mirabilis...

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Incidence of class A extended-spectrum -lactamases in Champagne-Ardenne (France): a 1 year prospective study

Abstract: A careful detection of bla(CTX-M)-type spread to other species would help to anticipate clonal endemics such as those observed in Enterobacter aerogenes TEM-24.

Cited by 51 publications

References 34 publications

Dissemination of Clonally RelatedEscherichia coliStrains Expressing Extended-Spectrum β-Lactamase CTX-M-15

Dissemination of Clonally RelatedEscherichia coliStrains Expressing Extended-Spectrum β-Lactamase CTX-M-15

Comparison of Nine Phenotypic Methods for Detection of Extended-Spectrum β-Lactamase Production by Enterobacteriaceae

TEM-187, a New Extended-Spectrum β-Lactamase with Weak Activity in a Proteus mirabilis Clinical Strain

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