Incidence of
 Acinetobacter
 Species Other than
 A. baumannii
 among Clinical Isolates of
 Acinetobacter
 : Evidence for Emerging Species

Turton, Jane F.; Shah, Jayesh; Ozongwu, C.; Pike, Rachel

doi:10.1128/jcm.02467-09

Cited by 166 publications

(143 citation statements)

References 27 publications

Supporting

Mentioning

130

Contrasting

Unclassified

Order By: Relevance

“…However, in our experiments the fish showed congested internal organs, whereas the pale liver was observed in channel catfish infected with A. baumanii (29). It should be emphasised that significant numbers of A. lwoffii and A. johnsonii were also recovered from human specimens often associated with bacteriaemia (28). This fact indicates that fish may transmit these pathogens to humans.…”

Section: Discussioncontrasting

confidence: 45%

Acinetobacter johnsonii and Acinetobacter lwoffii - the emerging fish pathogens

Kozinska

Paździor

Pękała

et al. 2014

Bulletin of the Veterinary Institute in Pulawy

118

View full text Add to dashboard Cite

The aim of this study was to characterise Acinetobacter sp. isolated from fish. Eight isolates obtained from diseased rainbow trout and common carp cultured in Poland were analysed. The isolates were identified using API 20 NE system as Acinetobacter sp. Afterwards, they were identified by sequencing 16S rDNA gene fragment. The bacteria were identified as A. johnsonii (two isolates), A. lwoffii (two isolates), A. junii/johnsonii (one isolate), A. calcoaceticus (one isolate), and Acinetobacter sp. (two isolates). The drug resistance of isolates was examined. The majority of the isolates were resistant to ampicilin, amoxicillin, and cephalothin and all demonstrated sensitivity to fluoroquinolones, except of one isolate. Two isolates were selected for the experimental infection of trout and carp to confirm their pathogenicity. Experimentally infected fish showed disease symptoms similar to those observed in fish naturally infected with these bacteria. This is the first report concerning pathogenicity of A. johnsonii for rainbow trout and A. lwoffii for common carp. These bacteria were regarded as emerging opportunistic pathogens of fish farmed in Poland. Acinetobacter strains are commonly known as microorganisms transmitting the antibiotic resistance genes. Therefore, they might have a great impact on the resistance transfer in aquaculture.

show abstract

Section: Discussioncontrasting

confidence: 45%

Acinetobacter johnsonii and Acinetobacter lwoffii - the emerging fish pathogens

Kozinska

Paździor

Pękała

et al. 2014

Bulletin of the Veterinary Institute in Pulawy

118

View full text Add to dashboard Cite

show abstract

Section: Resultsmentioning

confidence: 99%

“…Most Acinetobacter species are non-pathogenic or opportunistic, with significant research efforts focused on the frequently antibiotic-resistant, opportunistic pathogen Acinetobacter baumannii. However, opportunistic infections are also caused by Acinetobacter lwoffii, Acinetobacter ursingii (Turton et al, 2010) or strains in the Acinetobacter calcoaceticus-A. baumannii (Acb) complex (Gerner-Smidt et al, 1991), most commonly by genomic groups 13TU and 3 (Chuang et al, 2011;Karah et al, 2011) which correspond to the species Acinetobacter nosocomialis and Acinetobacter pittii, respectively (Nemec et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

“…The presence of intact umuDC or umuDAb-umuC operons typically associated with UV-induced mutagenesis (Nohmi, 2006) in half of all sequenced A. baumannii strains, and nine of 11 other Acinetobacter strains, however, suggests that many Acinetobacter species may be mutagenic upon UV exposure. As A. baumannii, other Acb group members and even non-Acb group species are associated with nosocomial infections (Turton et al, 2010), it is critical to understand whether this genus has the capacity to mutate in response to DNA-damaging events that the bacteria are likely to encounter, such as UV light or antibiotic exposure.Acinetobacter strains belonging to different recognized phenotypic clusters (Acb group, haemolytic species) were assayed for UV-induced mutagenesis activity by measuring the increase in the frequency of resistance to rifampicin after exposure to UV-C light and subsequent overnight growth. Additionally, all of the umuD or umuDAb alleles (types A-D) shown in Table 2 were represented in the strains tested.…”

mentioning

confidence: 99%

“…There was no association of UV-induced mutagenesis with the drug-resistance status of the A. baumannii strains (Table 2), nor were there significant homology differences among the umuDC loci of these three strains. However, the observation that the first and third most common pathogens in this genus (A. baumannii and A. ursingii, respectively;Turton et al, 2010) can undergo mutagenesis after DNA damage is crucial to understanding the evolution of these emerging pathogens.A. ursingii is notable for having three umuD genes: umuDAb-dinP and two type D umuDC loci.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Diverse responses to UV light exposure in Acinetobacter include the capacity for DNA damage-induced mutagenesis in the opportunistic pathogens Acinetobacter baumannii and Acinetobacter ursingii

et al. 2012

View full text Add to dashboard Cite

Error-prone and error-free DNA damage repair responses that are induced in most bacteria after exposure to various chemicals, antibiotics or radiation sources were surveyed across the genus Acinetobacter. The error-prone SOS mutagenesis response occurs when DNA damage induces a cell's umuDC-or dinP-encoded error-prone polymerases. The model strain Acinetobacter baylyi ADP1 possesses an unusual, regulatory umuD allele (umuDAb) with an extended 59 region and only incomplete fragments of umuC. Diverse Acinetobacter species were investigated for the presence of umuDC and their ability to conduct UV-induced mutagenesis. Unlike ADP1, most Acinetobacter strains possessed multiple umuDC loci containing either umuDAb or a umuD allele resembling that of Escherichia coli. The nearly omnipresent umuDAb allele was the ancestral umuD in Acinetobacter, with horizontal gene transfer accounting for over half of the umuDC operons. Despite multiple umuD(Ab)C operons in many strains, only three species conducted UV-induced mutagenesis: Acinetobacter baumannii, Acinetobacter ursingii and Acinetobacter beijerinckii. The type of umuDC locus or mutagenesis phenotype a strain possessed was not correlated with its error-free response of survival after UV exposure, but similar diversity was apparent. The survival of 30 Acinetobacter strains after UV treatment ranged over five orders of magnitude, with the Acinetobacter calcoaceticus-A. baumannii (Acb) complex and haemolytic strains having lower survival than non-Acb or non-haemolytic strains. These observations demonstrate that a genus can possess a range of DNA damage response mechanisms, and suggest that DNA damage-induced mutation could be an important part of the evolution of the emerging pathogens A. baumannii and A. ursingii. INTRODUCTIONWhen bacteria are exposed to DNA-damaging agents, such as UV light or chemical mutagens, a wide variety of SOS response genes (genes that play critical roles in repairing damaged DNA) are specifically induced (Friedberg et al., 1995;Walker, 1996). In the model developed by experimentation in Escherichia coli, SOS genes are negatively regulated by the LexA repressor binding to the SOS box in the promoter (Mount et al., 1972). DNA damage causes the activation of RecA, which facilitates LexA self-cleavage and releases the repression of SOS genes such as umuDC (Little et al., 1980;1981). UmuD initially causes a pause in cell division while DNA repair and replication occurs (Opperman et al., 1999). However, within minutes, UmuD homodimerizes and carries out intermolecular self-cleavage, facilitated by activated RecA* interaction (Nohmi et al., 1988). Two cleaved UmuD9 molecules then associate with UmuC to form DNA polymerase V, which carries out error-prone, trans-lesion DNA replication (SOS mutagenesis) (Reuven et al., 1999;Tang et al., 1999). Another errorprone polymerase commonly involved in SOS mutagenesis as part of the DNA damage response is DinP (also called DinB), DNA polymerase IV, which can function either by itself (Kim et al., 1997) or with U...

show abstract

Acinetobacter

Nemec

2022

Bergey's Manual of Systematics of Archaea and Bacteria

View full text Add to dashboard Cite

A.ci.ne.to.bac'ter. Gr. masc. adj. akinetos unable to move; N.L. masc. adj. bacter , a rod; N.L. masc. n. Acinetobacter , a non‐motile rod. Proteobacteria / Gammaproteobacteria / Pseudomonadales / Moraxellaceae / Acinetobacter Rods 0.9–1.6 × 1.5–2.5 μm, becoming spherical in the stationary phase of growth. Colonies are generally nonpigmented and are mucoid when the cells are encapsulated. Cells commonly occur in pairs. Do not form spores. Gram‐stain‐negative. Flagella are absent, and swimming motility does not occur, but cells display twitching motility associated with fimbriae. Strictly aerobic. Oxidase‐negative. Catalase‐positive. Mesophilic. Most strains do not reduce nitrate to nitrite in conventional assays. Grow well on complex media. Most strains grow in defined media containing a single carbon and energy source (most commonly acetate), using ammonium or nitrate salts or one of several common amino acids, as a source of nitrogen. The genome consists of a single circular chromosome sized 2.6−4.7 Mb and a strain‐dependent set of plasmid replicons. Widespread in nature, inhabiting diverse soil and water ecosystems and being associated with plants and animals. Some species reside on the human skin and mucosa. Several species can cause nosocomial infections; strains involved are often resistant to multiple antibiotics and can give rise to epidemics. In 2021, the genus included 73 species with correct names. DNA G + C content (mol%) : 34.9−49.6 (median: 40.2). Type species : Acinetobacter calcoaceticus Baumann et al. 1968b AL (basonym: Micrococcus calcoaceticus Beijerinck 1911) emend. Bouvet and Grimont 1986.

show abstract

Incidence of Acinetobacter Species Other than A. baumannii among Clinical Isolates of Acinetobacter : Evidence for Emerging Species

Cited by 166 publications

References 27 publications

Acinetobacter johnsonii and Acinetobacter lwoffii - the emerging fish pathogens

Acinetobacter johnsonii and Acinetobacter lwoffii - the emerging fish pathogens

Diverse responses to UV light exposure in Acinetobacter include the capacity for DNA damage-induced mutagenesis in the opportunistic pathogens Acinetobacter baumannii and Acinetobacter ursingii

Acinetobacter

Contact Info

Product

Resources

About