Incidence of metallo-beta-lactamase-producing  Pseudomonas aeruginosa  in diabetes and cancer patients

Varaiya, A; Kulkarni, M; Bhalekar, Pallavi; Dogra, J

doi:10.4103/0377-4929.41683

Cited by 81 publications

(92 citation statements)

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“…Though imipenem was found unaffected by the action of the enzymes in many studies, MBL production in our study was 15.38% which is comparable with the studies of Ibukun et al, [11] Prashant et al, [15] Agarwal et al, [16] Jayakumar et al, [18] Navneeth et al, [19] and, slightly raised level of carbapenem resistance were reported by Variya et al, (25%) [20]. The percentage variation in the resistance mechanism could be due to the study environment where the study was done.…”

Section: Discussionsupporting

confidence: 89%

Resistance Pattern of Pseudomonas aeruginosa in a Tertiary Care Hospital of Kanchipuram, Tamilnadu, India

2014

JCDR

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Purpose:This study was undertaken to analyze the extended spectrum of β lactamase (ESBL), metallo β lactamase (MBL) & AmpC production in Pseudomonas aeruginosa in various clinical samples. Materials & Methods:One hundred four non repetitive clinical specimens were inoculated onto nutrient agar, blood agar and incubated at 37 o C overnight. The colonies were tested for oxidase test and other biochemical tests and antibiogram. ESBL screening was done using 3 rd generation cephalosporins and confirmatory combined double disc test, imipenem-EDTA double disc synergy test for MBL enzyme and AmpC test using Cefoxitin disc. Results & Analysis:Out of 104 P.aeruginosa isolates, 42.30% were ESBL producer, 15.38 % MBL producer and none were AmpC producer. Imipenem, Ofloxacin, and aminoglycosides (amikacin (29.8%) tobramycin (29.8%) and netilmycin (13.46%) has got the better antipseudomonal activity in this study. 43 (41.35%) P.aeruginosa was found to be Multi Drug Resistant (MDR). Conclusion:This study highlights the prevalence of ESBL, MBL and MDR P.aeruginosa. Carbapenems and aminoglycosides are promising drugs with antipseudomonal activity in our study.

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Section: Discussionsupporting

confidence: 89%

Resistance Pattern of Pseudomonas aeruginosa in a Tertiary Care Hospital of Kanchipuram, Tamilnadu, India

2014

JCDR

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“…Urology 2(2%) 1(3) 3(27) 2(28.5) In this study, out of the 112 Pseudomonas aeruginosa isolates, 11(10%) were imipinem resistant strains, which was less than that observed by (Varaiya et al, 2008) and (Manoharan et al, 2010) This may be due to the minimal use of carbapenems in our hospital. Of the 11 imipinem resistant strains, 7 were found to be MBL producing isolates.…”

Section: Resultsmentioning

confidence: 59%

“…The IMP and VIM genes responsible for MBL production are horizontally transferable via plasmids and can rapidly spread to other bacteria (Nordmann and Poirel, 2002;Varaiya et al, 2008). So the occurrence of MBL positive isolates in a hospital setting not only pose a therapeutic problem, but also it is a serious concern for infection control management (Pitout et al, 2005).…”

Section: Issn: 2319-7706 Volume 7 Number 04 (2018)mentioning

confidence: 99%

Prevalence of ESBL and MBL Producing Pseudomonas aeruginosa in a Tertiary Care Hospital in Tirunelveli

Jeyabharathi¹,

Rejitha²

2018

Int.J.Curr.Microbiol.App.Sci

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“…Carbapenem antibiotics, including imipenem and meropenem, are used in the treatment of infections caused by P. aeruginosa (12); however, carbapenem resistance among P. aeruginosa strains has been reported in recent years (13). The production of β-lactamase and metallo-β-lactamase (MBL), and reduced penetration of the drugs are challenging factors in the therapeutic management of these infections (13,14).…”

Section: Introductionmentioning

confidence: 99%

Characterization by phenotypic and genotypic methods of metallo-β-lactamase-producing Pseudomonas aeruginosa isolated from patients with cystic fibrosis

Zhang

Wang

et al. 2014

Molecular Medicine Reports

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Abstract.Pseudomonas aeruginosa continues to be a predominant cause of infections with high intrinsic resistance to antibiotics, resulting in treatment failure. P. aeruginosa is the leading cause of respiratory infections among cystic fibrosis (CF) patients. Resistance to carbapenem antibiotics among P. aeruginosa has been reported. Thus, this study was undertaken to characterize the metallo-β-lactamase (MBL) production of P. aeruginosa by phenotypic and genotypic methods. A total of 572 sputum samples were collected from cystic fibrosis patients along with the patient demographic details in a questionnaire. In total, 217 P. aeruginosa isolates were collected and an antibiogram revealed that 159 (73.3%) and 141 (64.9%) of these colonies exhibited resistance to imipenem and meropenem, respectively. Ceftazidime and tobramycin resistance were both identified in 112 (51.6%) isolates, and resistance to piperacillin-tazobactam, gatifloxacin and netilmicin was detected in 96 (44.2%) respective samples. A total of 62 (28.6%) respective samples were resistant to cefoperazone, cefepime and ceftriaxone. The least antibiotic resistance was shown to amikacin and ceftizoxime with 51 (23.5%) and 32 (14.7%) respective colonies resistant to the antibiotics. The minimum inhibitory concentration (MIC) for imipenem revealed a reduction in the MIC values. MBL screening by the zone enhancement method using ceftazidime plus EDTA discs demonstrated that 63 (56.25%) of the colonies were positive for MBL. A total of 53 (84.1%) samples expressed blaVIM and 48 (76.1%) expressed blaIMP genes, as detected by duplex polymerase chain reaction. In conclusion, carbapenem resistance is of great clinical concern in cystic fibrosis patients with P. aeruginosa infection. Therefore, mandatory regular screening and monitoring the resistance in P. aeruginosa among CF patients is required.

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Incidence of metallo-beta-lactamase-producing Pseudomonas aeruginosa in diabetes and cancer patients

Cited by 81 publications

References 0 publications

Resistance Pattern of Pseudomonas aeruginosa in a Tertiary Care Hospital of Kanchipuram, Tamilnadu, India

Resistance Pattern of Pseudomonas aeruginosa in a Tertiary Care Hospital of Kanchipuram, Tamilnadu, India

Prevalence of ESBL and MBL Producing Pseudomonas aeruginosa in a Tertiary Care Hospital in Tirunelveli

Characterization by phenotypic and genotypic methods of metallo-β-lactamase-producing Pseudomonas aeruginosa isolated from patients with cystic fibrosis

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