Incidence of (n-3) essential fatty acid deficiency on trout
reproductive processes

Leray, Claude; Nonnotte, G.; Roubaud, P; Léger, Claude

doi:10.1051/rnd:19850409

Cited by 58 publications

(30 citation statements)

References 13 publications

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“…VTG is a peculiar lipoprotein since it is characterized by a strong hydrophobicity ; however, it can be expected that, in relation with the length and degree of unsaturation, the arrangement of phospholipids and proteins also depends on the spatial organization of the acid chains. Alterations in embryonic development observed when vitellogenic trout were fed a n-3 deficient diet (Leray et al, 1985) (Owen and Gillett, 1983 ;Owen, McIntyre and Gillett, 1984). Moreover, it is quite possible that VTG PUFA modulate interactions between the complex and the plasma membrane of the oocyte and affect VTG incorporation since in trout, the yolk cycle presents the features of receptor-mediated endocytosis (Busson-Mabillot, 1984).…”

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confidence: 99%

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Biochemical analysis of vitellogenin from rainbow trout (Salmo gairdneri) : fatty acid composition of phospholipids

Frémont¹,

Riazi²

1988

Reprod. Nutr. Dévelop.

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Summary. Vitellogenin was obtained from three year-old vitellogenic trout.Two procedures of isolation were compared : dialysis against distilled water and ultracentrifugation in the density interval 1 .21 -1 .28 g/ml.Similar patterns were observed by gel filtration and electrophoresis for both preparations of vitellogenin, indicating that electric charge and molecular weight were not modified by either procedure.The apparent M, of the native form was 560,000 in gel filtration, whereas that of the monomer was estimated as 170,000 by sodium dodecylsulfate-polyacrylamide gel electrophoresis. Minor proteins were also detected (100,000-88,000-78,000).The main components were protein (79%), and lipids (19 %), Carbohydrates accounted for 0.3 % when protein phosphorus and calcium each represented 0.7 % of total weight.Phospholipids (70 % of total lipids) mainly consisted of phosphatidylcholine in which n-3 docosahexanenoic acid accounted for one-third of total fatty acids.The results show the high levels of essential fatty acids in structural lipids which are known to be involved in embryo development.Introduction.

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confidence: 99%

“…In addition, we characterized the phospholipid moiety of VTG which is the main vehicle for the essential fatty acids involved in the embryonic development of trout (Leray et al, 1985 (Gilfillan et al, 1983).…”

mentioning

confidence: 99%

Biochemical analysis of vitellogenin from rainbow trout (Salmo gairdneri) : fatty acid composition of phospholipids

Frémont¹,

Riazi²

1988

Reprod. Nutr. Dévelop.

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“…Siendo el aceite de pescado particularmente rico en DHA, se emplea como componente fundamental de los balanceados para peces y, en tales condiciones, Buzzi et ál. (33) Dada su importancia, estos ácidos se determinan en la mayoría de las evaluaciones sobre composición o metabolismo en general (16,26); la relación derivada DPA/DHA se convierte entonces en un factor de registro común y con utilidad comparativa entre diferentes trabajos. También tiende a ubicarse dentro de un rango relativamente estrecho, independiente de las condiciones experimentales y de determinación en laboratorio con, por ejemplo, .…”

Section: Discussionunclassified

“…Esta situación se hace evidente en condiciones experimentales en las que se promueven deficiencias de tipo extremo (26,29,30) dirigidas a identificar necesidades de ácidos grasos específicos. Aun así, Tocher (9) establece que la composición general, específicamente en los huevos, es más resistente a los cambios dietarios que aquella que se puede ocasionar sobre otro tipo de tejidos, en una regularidad de incorporación que particularmente sobre el DHA identifican Izquierdo (36) y Wiegand (6), explicándola como producto de una presión de selección y una selectividad en la retención durante la embriogénesis, de forma que se logra mantener dentro de rangos reducidos.…”

Section: Discussionunclassified

Composición de ácidos grasos en ovas de trucha arco iris (Oncorhynchus mykiss, Walbaum, 1792)

Puccini¹,

Parra²,

Gonzalez³

2012

Rev. Med. Vet.

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ResumenEn este artículo se analizan con parámetros de composición 58 puestas de trucha arcoiris obtenidas, manejadas y monitoreadas individualmente en desempeño desde la fertilización hasta finalizar la etapa de reabsorción de vesícula. Además de los valores reproductivos, el contenido de ácidos grasos en las ovas se determinó en fresco, mediante cromatografía de gases. En el perfil medio se destacan los ácidos palmítico (C16), oleico (C18:1n-9) y docosahexaenoico (C22:6n-3) como los más representativos, con casi el 60% del total y, en general, en concentraciones estables entre las hembras. Tanto para cada ácido determinado como para el conjunto de contenidos integrados de las series n-3 y n-6, y los colectivos de saturados (SAF), monoinsaturados (MUFA) y poliinsaturados (PUFA), se define un patrón de composición similar al reportado para la especie en otros esquemas de manejo y bajo regímenes nutricionales variables. Se analizó la condición conservativa en la incorporación de ácidos grasos al huevo, y se discutió su utilidad como posibles definitorios de calidad, teniendo como referente la alta variabilidad registrada en la supervivencia al final del proceso de incubación.Palabras clave: ácidos grasos, calidad del huevo, composición del huevo, Oncorhynchus mykiss. Fatty Acid Composition in Rainbow Trout Eggs (Oncorhynchus mykiss, Walbaum, 1792) AbstractThis article analyzes, with composition parameters, 58 rainbow trout egg-layings obtained, handled and monitored individually in performance from their fertilization until the end of the gallbladder reabsortion phase. Aside from the reproductive values, the content of fatty acids in the eggs was determined in fresh conditions through gas chromatography. The average profile highlights the palmitic acid (C16), oleic acid (C18:1n-9) and docosahexaenoic acid (C22:6n-3), as the most representative, with almost 60% of the total and, in general, in stable concentrations among females. Both for each determined acid and for the suite of integrated series n-3 and n-6, and the groups of saturated (SAF), monounstaturated (MUFA) and polyunsaturated (PUFA), a pattern of similar composition to the one reported is defined for the species in other management schemes and under varying nutritional regimes. The conservative condition was analyzed in the incorporation of fatty acids into the egg and its usefulness as possible quality definers was discussed, using the high variability recorded in survival at the end of the incubation process as reference.

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“…In clupeoid fishes, the EFA produced by phytoplankton are incorporated into the intermediate tissues, gonads, and eventually oocytes during the spawning season (Linko et al 1985, Shirai et al 2002, Garrido et al 2007, Castro et al 2010. Elevated levels of ARA, DHA, EPA, and the DHA/EPA ratio in oocytes accounted for an increased hatching success in a variety of fish species (Leray et al 1985, Pickova & Dutta 1997, Lane & Kohler 2006, Patterson & Green 2014, Asil et al 2017). Additionally, EFA play an important role in larval osmoregulation (Bransden et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Using oocyte essential fatty acid composition to assess spawner reproductive potential under hypersaline conditions

Döring¹,

Ekau²

2017

Mar. Ecol. Prog. Ser.

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Inversion of estuaries in the dry tropics (i.e. salinity increases upstream) against the backdrop of a globally changing climate is forcing migratory clupeid fish species to adapt quickly to modifications in their spawning habitats to ensure reproductive success. Hatching success and survival probabilities of marine fish eggs and early life stages are prone to impairment under high temperature and elevated salinity conditions. Essential fatty acids affect osmoregulation in marine fish early life stages, and deficiencies can therefore be expected to cause elevated offspring mortality under hyperosmotic conditions, ultimately modifying a stock's reproductive potential. Here we show that oocytes spawned by bonga shad Ethmalosa fimbriata inside a hypersaline estuary exhibited significantly higher 20:4 (n-6) proportions (1.6 ± 0.7% of total fatty acids) than oocytes spawned under marine conditions (0.6 ± 0.2%). Further, oocyte proportions in 20:4 (n-6), 20:5 (n-3), and 20:6 (n-3) were positively correlated with water temperature, water salinity, and female reproductive investment (relative batch fecundity, gonado-somatic index, oocyte dry weight). Oocytes spawned during high temperature/high salinity conditions inside the estuary are therefore likely to develop normally under the prevailing conditions. Reproductive potential was determined to be highest in females spawning inside the estuary at the end of the wet season, when temperatures are high and salinities are less stressful due to freshwater input. Results lead to the conclusion that migratory clupeid fishes can maintain their reproductive potential in hypersaline estuaries and lagoons, which potentially allows for sustainable stock sizes.

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Incidence of (n-3) essential fatty acid deficiency on trout reproductive processes

Cited by 58 publications

References 13 publications

Biochemical analysis of vitellogenin from rainbow trout (Salmo gairdneri) : fatty acid composition of phospholipids

Biochemical analysis of vitellogenin from rainbow trout (Salmo gairdneri) : fatty acid composition of phospholipids

Composición de ácidos grasos en ovas de trucha arco iris (Oncorhynchus mykiss, Walbaum, 1792)

Using oocyte essential fatty acid composition to assess spawner reproductive potential under hypersaline conditions

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