Incidence of respiratory viruses in preterm infants submitted to mechanical ventilation

The aim of this study was to determine if there was a correlation between respiratory syncytial virus (RSV) and metapneumovirus (MPV) genotypes and clinical data of Brazilian infants hospitalized for acute lower respiratory infection. The viruses in the patients' nasopharyngeal secretions were studied using the polymerase chain reaction and phylogenetic analysis. The study assessed 144 infants; 31.9% were RSV positive and 5.6% were MPV positive. Statistical analysis was performed using the chi-squared test, Fisher's test, Odds ratio, univariate logistic regression, non-conditional multivariate logistic regression and the forward -stepwise method. Multivariate analysis confirmed a significant relationship between a positive PCR test for RSV and hospitalization during the month of May and with pulse oximetry less than 90%. The phylogenetic analysis indicated the genotypes GA2, GA5, SAA1 (Group A), SAB1, SAB3 and BA (Group B) for RSV and Group B, subgroup B1, for MPV.

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“…In these studies, this virus was present in 17.4 -48% for RSV [27,28] and MPV in 6.4 -17.8% of the patients [4,29].…”

Section: Discussionmentioning

confidence: 90%

Genotypes and clinical data of respiratory syncytial virus and metapneumovirus in brazilian infants: a new perspective

et al. 2009

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“…Therefore, it is plausible that VRTIs acquired during the NICU period could worsen BPD in preterm infants although previous studies have mostly been small observational studies 12,13,[15][16][17][18][19] . We aimed to assess the association between nosocomial VRTIs acquired in the NICU and respiratory morbidity up to hospital discharge.…”

Section: Introductionmentioning

confidence: 99%

Outcomes of Nosocomial Viral Respiratory Infections in High-Risk Neonates

et al. 2016

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A note on versions:The version presented here may differ from the published version or from the version of record. If you wish to cite this item you are advised to consult the publisher's version. Please see the repository url above for details on accessing the published version and note that access may require a subscription.For more information, please contact eprints@nottingham.ac.uk Financial disclosure: The other authors have no financial relationships relevant to this article to disclose. Outcomes of nosocomial viral respiratory infections in high-risk neonates Conflicts of interest:The other authors have no potential conflicts of interest to disclose. Abbreviations:VRTIs -viral respiratory tract infections BPD -bronchopulmonary dysplasia NICU -neonatal intensive care unit NEC -necrotising enterocolitis CPAP -continuous positive airway pressure Bi-PAP -bi-level positive airway pressure HFOV -high frequency ventilation ECMO -extracorporeal membrane oxygenation RT-PCR -real time polymerase chain reaction RVP -respiratory viral panel NHS -National Health Service What's Known on This SubjectViral respiratory tract infections cause severe respiratory morbidity in ex-preterm infants after NICU discharge. They are now recognized to be more prevalent in the NICU but their longterm impact, prior to discharge, during this early period of life is unclear. What This Study AddsThis study identifies the adverse impact viral respiratory infections, particularly rhinovirus, have on newborn infants during their initial NICU admission. Identification of the associated significant respiratory morbidity and healthcare costs should focus efforts on reducing these nosocomially acquired infections. Contributors' Statements:Dr Don Sharkey conceptualized and designed the study, drafted the initial manuscript, and approved the final manuscript as submitted.Drs Shairbanu Zinna, Arthi Lakshmanan, Shin Tan, Shiu Soo, Lisa Szatkowski and Miss Rebecca McClaughry and Mr Martin Clarkson carried out the data collection, initial analyses, reviewed and revised the manuscript, and approved the final manuscript as submitted.All authors approved the final manuscript as submitted and agree to be accountable for all aspects of the work. ABSTRACTBackground Neonatal respiratory disease, particularly bronchopulmonary dysplasia, remains one of the leading causes of morbidity and mortality in newborn infants. There is increasing evidence that nosocomially acquired viral respiratory tract infections (VRTIs) are not uncommon in the neonatal intensive care unit but there are few studies on their impact on neonatal respiratory outcomes and the associated healthcare costs. This study aimed to assess the association between nosocomial VRTI and neonatal respiratory disease.

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“…The respiratory syncytial virus (RSV) is considered to be the most common viral agent of lower respiratory tract infections in infants and young children, and can cause serious respiratory illnesses 5,7,12,20 .The highest morbidity rates are observed in infants, in the elderly and in the immunocompromised 4,9,17,18,22 . Viral isolation in cell cultures continues to be considered the most definitive test for diagnosis 6 .…”

Section: Introductionmentioning

confidence: 99%

Comparison of direct immunofluorescence, conventional cell culture and polymerase chain reaction techniques for detecting respiratory syncytial virus in nasopharyngeal aspirates from infants

Reis

Fink

Machado

et al. 2008

Rev. Inst. Med. trop. S. Paulo

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SUMMARYA total of 316 samples of nasopharyngeal aspirate from infants up to two years of age with acute respiratory-tract illnesses were processed for detection of respiratory syncytial virus (RSV) using three different techniques: viral isolation, direct immunofluorescence, and PCR. Of the samples, 36 (11.4%) were positive for RSV, considering the three techniques. PCR was the most sensitive technique, providing positive findings in 35/316 (11.1%) of the samples, followed by direct immunofluorescence (25/316, 7.9%) and viral isolation (20/315, 6.3%) (p < 0.001). A sample was positive by immunofluorescence and negative by PCR, and 11 (31.4%) were positive only by RT-PCR. We conclude that RT-PCR is more sensitive than IF and viral isolation to detect RSV in nasopharyngeal aspirate specimens in newborn and infants.

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Incidence of respiratory viruses in preterm infants submitted to mechanical ventilation

Cited by 34 publications

References 29 publications

Genotypes and clinical data of respiratory syncytial virus and metapneumovirus in brazilian infants: a new perspective

Genotypes and clinical data of respiratory syncytial virus and metapneumovirus in brazilian infants: a new perspective

Outcomes of Nosocomial Viral Respiratory Infections in High-Risk Neonates

Comparison of direct immunofluorescence, conventional cell culture and polymerase chain reaction techniques for detecting respiratory syncytial virus in nasopharyngeal aspirates from infants

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