Incidence of Staphylococci and Streptococci During Winter in Mastitic Milk of Sahiwal Cow and Murrah Buffaloes

Abstract

Mastitis is a serious problem in dairy sector and among various aetiological agents, the incidence of staphylococci and streptococci remains high in milking animal. The present study was focused on detection of staphylococci and streptococci in winter season. Milk samples (117) of mastitic animals were tested for presence of staphylococci and streptococci using biochemical and PCR based assays. The testing revealed majority of animals (90.6%) were infected with more than one causative agent. Amongst 117 sample, 109 and 90 comprised of staphylococci and 90 streptococci, respectively. Distribution proportion of S. aureus, S. epidermidis, S. agalactiae, S. uberis and S. dysgalactiae among the mastitic cases was found as 64.9, 7.7, 5.1, 1.7, 48.7, 65.8 and 0.8%, respectively. Streptococci and staphylococci were observed in different combinations and the frequent were S. aureus/S. agalactiae/S. uberis, S. aureus/S. uberis, S. aureus/S. agalactiae and S. agalactiae/S. uberis which were accounted for 23.9, 19.7, 5.9 and 2.6%, respectively. Approximately half of the (52.1%) cases were observed for reoccurrence of mastitis. Reoccurrence of mastitis in winter season among these cases was significantly low as compared to summer (cattle-5 cases; buffaloes-2 cases). In addition, prevalence of S. aureus, S. agalactiae, S. uberis, and S. epidermidis in reoccurring mastitic cases was 73.7, 63.9, 45.9 and 6.6%, respectively. The observations revealed mastitis causing pathogens remains in hidden phase in winter season; however, cannot be neglected. The observation might be helpful in culling or segregation of cows for mastitis reduction programmes.

Isolation and characterization of mastitis pathogens and milk composition changes in Murrah buffaloes (Bubalus bubalis) during winter season

“…The relative prevalence rates of various bacterial strains revealed that S. aureus (35.29%) was the major causative pathogen of bovine mammary gland followed by S. agalactiae (25.49%). Similar results have also been reported by many researchers (Khan and Muhammad, 2005;Ericission et al ,2009;Sentitula et al, 2012;Duguma et al, 2014 ;Z. Ali et al,2015;Galvan et al, 2015).…”

Microbiological Evaluation of Milk Quality and Antibiogram in Dairy Cows Managed on Pasture

“…corresponded to 38.76% of the total agents isolated from milk samples. clinical and subclinical mastitis (Li et al, 2017;Yadav and Kumar, 2012). These findings corroborate those presented in this study, which demonstrate that Staphylococcus aureus was the main agent isolated (41.65%), followed by enterobacteria (37.50%).…”

Occurrence and aetiology of subclinical mastitis in water buffalo in Bangladesh

“…Other studies in India have reported higher occurrence (59–78%) (Bhanot et al ., 2012; Charaya et al ., 2015), while lower prevalence (28%) has been reported in a small-scale study in Bangladesh (Islam et al ., 2019). Compared with the quarter-level prevalence, the animal-level SCM prevalence was much higher in this study (81.6%), which is in line with previously reported values ranging from 70 to 78% (Bachaya et al ., 2005; Sentitula et al ., 2012). These differences in estimated SCM could be due to differences between study sites, study years, geographical areas, the criteria applied for SCM detection, climate variations, and underlying animal- or quarter-level risk factors (Hussain et al ., 2018).…”