Background/Aim: The aim of this study was a CBCT radiographic bone density analysis of bicortical defects healing when treated with guided bone regeneration and two bone substitutes-bovine xenograft and alloplastic bone substitute. Methods. Research was performed on domestic pigs in two phases. In the first phase, extraction of all teeth in the inter-canine sector was performed in the lower jaw and postextraction wounds were sutured. In the second phase, after the period required for healing, bi-cortical defects were formed-following elevation of mucoperiosteal flaps from the vestibular and lingual side in the area of the previously extracted teeth, surgical removal of the cambium layer of periosteum was performed in the area of future defects with sharp surgical scissors and curette. Two defects, 10 mm in diameter, on the left and right side of the medial line were formed and filled with alloplastic bone substitute on left and xenograft on right side, afterwards. After augmentation, the defects were covered by collagen resorptive membrane on both sides and flap was repositioned and sutured. After the 12 weeks, experimental animals were sacrificed. Results. Analysis of bone tissue showed a statistically significant difference between the examined substitutes (p<0.01). After applying Bonfferoni correction, the difference was still statistically significant. Conclusion: Both bone substitutes used in the study showed good osteoconductive properties in the treatment of bi-cortical defects. Bone tissue density in defects filled with alloplastic bone graft was statistically significantly higher than that in the defects filled with xenograft.