Inclusion Complex of Docetaxel with Sulfobutyl Ether β-Cyclodextrin: Preparation, In Vitro Cytotoxicity and In Vivo Safety

Abstract

Docetaxel (DTX), as a first-line anti-tumor drug, has been studied for decades for its diverse bioactivities. However, DTX presents poor solubility in water, low bioavailability and serious toxic side effects which has hindered its application in the clinic. To address these problems, docetaxel-sulfobutyl ether-β-cyclodextrin inclusion complex (DTX-SBE-β-CD) was prepared successfully by saturated aqueous solution method. Sulfobutyl ether β-cyclodetrin (SBE-β-CD) is used as delivery material. For this study, the inclusion complex of docetaxel with sulfobutyl ether β-cyclodetrin (DTX-SBE-β-CD) was prepared and optimized its properties to enhance the cytotoxicity of cancer cells. A large number of physical characterization results showed that DTX-SBE-β-CD inclusion complex was successfully prepared by saturated aqueous solution method. DTX-SBE-β-CD inclusion complex was optimized by Central Composite Design. DTX-SBE-β-CD had an inhibitory effect on the in vitro determination of MCF-7 and HepG2 cells by MTT assay. Pharmacokinetic studies were carried out on male Sprague–Dawley rats by tail injection, including the distribution, metabolism and elimination of DTX-SBE-β-CD in vivo. In the experimental study of inhibition of cancer cells, DTX and DTX-SBE-β-CD showed apparent concentration-dependent inhibitory actions on tumor cells and the inhibition of DTX-SBE-β-CD group was more obvious.

Synergistic Antitumor Potency of a Self-Assembling Cyclodextrin Nanoplex for the Co-Delivery of 5-Fluorouracil and Interleukin-2 in the Treatment of Colorectal Cancer

“…Hemolysis, liver damage, renal damage status in blood and histological data are used to evaluate the safety of polymers. The inclusion complex of SBE βCD prepared with docetaxel was found to be safer than free docetaxel in the hemolysis and vascular stimulation test in rabbits [ 50 ]. When the implant containing poly-HPβCD and ropivacaine was examined in the rat, no negative effects were observed in the samples and histopathological data [ 51 ].…”

Ternary inclusion complex of docetaxel using ß-cyclodextrin and hydrophilic polymer: Physicochemical characterization and in-vitro anticancer activity

“…Thus, it can be stated that β-CD is efficient in improving the solubility of docetaxel. An earlier complexation study performed using sulfobutyl ether β-cyclodextrin showed that docetaxel also formed a stable inclusion complex with it, which also showcased a magnificent increase in water solubility (Ren et al, 2020). This solubility has been further enhanced by the addition of the hydrophilic polymer.…”

3D-Printed Drug-Loaded Composite Scaffolds to Promote Osteogenesis and Antibacterial Activity

“…Following the previous study, we used the saturated aqueous solution method to prepare the β-CD-CHX. The β-CD was dissolved in double-distilled water and the CHX in ethanol, and the molecular molar ratio of β-CD to CHX was 1:1. , The next step is the preparation of scaffolds. First, CAD software was used to design the scaffold material.…”