Incorporation and Antibody Recognition of a Lipid-Anchored Membrane Protein in Supported Lipid Layers

A Silicate Gel for Promoting Deposition of Lipid Bilayers

“…These bilayers are simple models of biological membranes and can be used in combination with various transducers for the study of membrane proteins and processes (4)(5)(6)(7)(8). In order for SLBs to be useful for the study of membrane proteins it is essential that they should be able to incorporate the membrane proteins to be studied; this has been shown, for some proteins, to necessitate an aqueous layer between the bilayer and the underlying substrate to accommodate extra-membrane protein domains.…”

“…A number of surface-sensitive techniques have been used to study the interaction of vesicle suspensions with solid surfaces, including electrochemical (10) and optical (5,(11)(12)(13) methods. Recently, acoustic wave devices have also been applied to these studies and have been shown to be able to monitor the mass and viscoelastic changes occurring during vesicle fusion on the device surface (14)(15)(16).…”

Silica supported phospholipid layers doped with GM1: A comparison between different methods

“…Most of the reports found in the literature deal with formation of phospholipid layers on different hydrophilic solid supports, e.g. silica [3][4][5][6][7], mica [8][9][10], gold [3,11] but only a limited amount of research has been devoted to hydrophobized surfaces (mainly hydrophobized gold) [3,[11][12][13]. Apart from the above solid supports, phospholipid monolayers have also been reported on a mercury drop surface [14].…”

“…The aim of the present work was therefore to develop a reproducible method to form stable and close-packed lipid monolayers onto hydrophobized silica surfaces, which are resistant to both buffer rinsing and detergent washing. Several methods for supported lipid layer formation onto hydrophilic surfaces are described in the literature, namely, Langmuir-Blodgett transfer [8,9], spin coating [16], vesicle fusion [3,4,8,[10][11][12]17], and deposition from mixed micelles [5,6,13]. The methods to form the lipid layers selected in this study were vesicle fusion, since it was found to be the most common method, adsorption of mixed micelles, due to the fact that little work has been devoted to their use, and the so called hydration method (deposition from solvent), which is normally employed in the ELISA assays [18,19].…”

Utilizing adsorbed proteoliposomes trapped in a non-ruptured state on SiO2 for amplified detection of membrane proteins

“…Such structures are, for example, supported planar lipid membranes (Sackmann, 1996). Supported planar lipid membranes have the ability to act as an inert background, thus reducing non-specific interactions (Glasmästar et al, 2002;Svedhem et al, 2003) which, when combined with the possibility to incorporate defined biological functionalities constitutes a platform for specific biorecognition events to be detected (Gizeli et al, 1997;McConnell et al, 1986;Liley et al, 1997;Salafsky et al, 1996). In addition, supported membranes are generic sensor templates in the sense that they, in contrast to other surface-modification protocols, are compatible with both transmembrane proteins, a class of protein important as targets in drug design (Groves, 2002) and water-soluble biomolecules, generally anchored to the membrane via chemically modified lipids (Schmidt et al, 1992;Snejdorova et al, 1993;Puu and Gustafson, 1997;Puu et al, 2000;Siontorou et al, 2000;Liebau et al, 2001).…”