Incorporation of camptothecin into N-phthaloyl chitosan-g-mPEG self-assembly micellar system

Opanasopit, Praneet; Ngawhirunpat, Tanasait; Chaidedgumjorn, Amornrut; Rojanarata, Theerasak; Apirakaramwong, Auayporn; Phongying, Sasiprapha; Choochottiros, Chantiga; Chirachanchai, Suwabun

doi:10.1016/j.ejpb.2006.06.001

Cited by 88 publications

(47 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Various approaches have been reported in literature in order to reduce these severe systemic toxicities and enhance antitumor effects of CPT. These includes conjugation to synthetic polymers [28][29][30][31], incorporation into liposomes [32,33], microspheres [34,35], nanohybrids [36], and polymeric micelles [37][38][39][40][41].…”

Section: Introductionmentioning

confidence: 99%

Mixed PEG–PE/vitamin E tumor-targeted immunomicelles as carriers for poorly soluble anti-cancer drugs: Improved drug solubilization and enhanced in vitro cytotoxicity

Sawant

Torchilin

2008

European Journal of Pharmaceutics and Biopharmaceutics

View full text Add to dashboard Cite

Two poorly soluble, potent anticancer drugs, paclitaxel and camptothecin, were successfully solubilized by mixed micelles of polyethylene glycol-phosphatidyl ethanolamine (PEG-PE) and vitamin E. Drug containing micelles were additionally modified with anti-nucleosome monoclonal antibody 2C5 (mAb 2C5), which can specifically bring micelles to tumor cells in vitro. The optimized micelles had an average size of about 13-to-22 nm and the immuno-modification of micelles did not significantly change it. The solubilization of both drugs by the mixed micelles was more efficient than by micelles made of PEG-PE alone. Solubilization of camptothecin in micelles prevented also the hydrolysis of active lactone form of the drug to inactive carboxylate form. Drug loaded mixed micelles and mAb 2C5-immunomicelles demonstrated significantly higher in vitro cytotoxicity than free drug against various cancer cell lines.

show abstract

Section: Introductionmentioning

confidence: 99%

Mixed PEG–PE/vitamin E tumor-targeted immunomicelles as carriers for poorly soluble anti-cancer drugs: Improved drug solubilization and enhanced in vitro cytotoxicity

Sawant

Torchilin

2008

European Journal of Pharmaceutics and Biopharmaceutics

View full text Add to dashboard Cite

show abstract

“…[19][20][21] Various nanoparticles have been developed to encapsulate camptothecin to decrease its interaction with serum albumin, which enhances the stability of the drug. [22][23][24] In our results, LPPC-encapsulated BP retained its cytotoxic activity even when incubated in a protein-rich, oxygenic, or acid/alkaline solution (Figure 2). Collectively, the results showed that LPPC encapsulation protects BP against serum protein interactions, conformational changes, and oxidation.…”

mentioning

confidence: 59%

Liposomal n-butylidenephthalide protects the drug from oxidation and enhances its antitumor effects in glioblastoma multiforme

Lin¹,

Chang²,

Huang³

et al. 2015

IJN

View full text Add to dashboard Cite

Background The natural compound n -butylidenephthalide (BP) can pass through the blood–brain barrier to inhibit the growth of glioblastoma multiforme tumors. However, BP has an unstable structure that reduces its antitumor activity and half-life in vivo. Objective The aim of this study is to design a drug delivery system to encapsulate BP to enhance its efficacy by improving its protection and delivery. Methods To protect its structural stability against protein-rich and peroxide solutions, BP was encapsulated into a lipo-PEG-PEI complex (LPPC). Then, the cytotoxicity of BP/LPPC following preincubation in protein-rich, acid/alkaline, and peroxide solutions was analyzed by MTT. Cell uptake of BP/LPPC was also measured by confocal microscopy. The therapeutic effects of BP/LPPC were analyzed in xenograft mice following intratumoral and intravenous injections. Results When BP was encapsulated in LPPC, its cytotoxicity was maintained following preincubation in protein-rich, acid/alkaline, and peroxide solutions. The cytotoxic activity of encapsulated BP was higher than that of free BP (~4.5- to 8.5-fold). This increased cytotoxic activity of BP/LPPC is attributable to its rapid transport across the cell membrane. In an animal study, a subcutaneously xenografted glioblastoma multiforme mouse that was treated with BP by intratumoral and intravenous administration showed inhibited tumor growth. The same dose of BP/LPPC was significantly more effective in terms of tumor inhibition. Conclusion LPPC encapsulation technology is able to protect BP’s structural stability and enhance its antitumor effects, thus providing a better tool for use in cancer therapy.

show abstract

“…Thus, the internal core can serve as a nano-container for hydrophobic drugs. Modified chitosan is appropriate for decreasing severe side effects such as cytotoxicity in usual tissue (Fang et al, 2006;Gorochovceva et al, 2005;Opanasopit et al, 2006).…”

Section: Modified Chitosan and Chitinmentioning

confidence: 99%

Nanoparticles Based on Modified Polysaccharides

Namazi¹,

Fathi²,

Heydari³

2012

The Delivery of Nanoparticles

View full text Add to dashboard Cite

Incorporation of camptothecin into N-phthaloyl chitosan-g-mPEG self-assembly micellar system

Cited by 88 publications

References 28 publications

Mixed PEG–PE/vitamin E tumor-targeted immunomicelles as carriers for poorly soluble anti-cancer drugs: Improved drug solubilization and enhanced in vitro cytotoxicity

Mixed PEG–PE/vitamin E tumor-targeted immunomicelles as carriers for poorly soluble anti-cancer drugs: Improved drug solubilization and enhanced in vitro cytotoxicity

Liposomal n-butylidenephthalide protects the drug from oxidation and enhances its antitumor effects in glioblastoma multiforme

Nanoparticles Based on Modified Polysaccharides

Contact Info

Product

Resources

About