Incorporation of gelatin microparticles on the formation of adipose-derived stem cell spheroids

Kim, Yun-Kyung; Baipaywad, Phornsawat; Jeong, Younghyen; Park, Hansoo

doi:10.1016/j.ijbiomac.2018.01.046

Cited by 24 publications

(22 citation statements)

References 37 publications

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“…Incorporation of growth factor-loaded microparticles in the developing cell spheroid prevents the formation of a necrotic core by effectively delivering nutrients at the center of the spheroids. 95 Additionally, GMPs can be loaded with specific cytokines for the directed differentiation of the cell spheroids. In one study, GMPs were utilized to deliver TGF-b1 into bone marrow-derived cell spheroids for the induction of chondrogenesis for cartilage tissue engineering.…”

Section: Gelatin-based 3d Microenvironment For Cell Culturementioning

confidence: 99%

“…Composite microparticles overcome limitations of gelatin and other types of biomaterial. Other commonly used materials in generating microparticles include inorganic polymers (hydroxyapatite, calcium phosphate), 142,143 organic polymers (poly-lactic-co-glycolic acid, poly-lactic acid, and poly-ethylene glycol), [144][145][146] natural proteins (collagen, ECM, and gelatin), 95,147,148 and polysaccharides (alginate, chitosan). [148][149][150] The use of these materials for the generation of microparticles have their own advantages and drawbacks.…”

Section: Fabrication Of Gelatin Nano and Microparticlesmentioning

confidence: 99%

See 1 more Smart Citation

Engineering and Functionalization of Gelatin Biomaterials: From Cell Culture to Medical Applications

Bello

Kim

et al. 2020

Tissue Engineering Part B: Reviews

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399

259

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Health care and medicine were revolutionized in recent years by the development of biomaterials, such as stents, implants, personalized drug delivery systems, engineered grafts, cell sheets, and other transplantable materials. These materials not only support the growth of cells before transplantation but also serve as replacements for damaged tissues in vivo. Among the various biomaterials available, those made from natural biological sources such as extracellular proteins (collagen, fibronectin, laminin) have shown significant benefits, and thus are widely used. However, routine biomaterial-based research requires copious quantities of proteins and the use of pure and intact extracellular proteins could be highly cost ineffective. Gelatin is a molecular derivative of collagen obtained through the irreversible denaturation of collagen proteins. Gelatin shares a very close molecular structure and function with collagen and thus is often used in cell and tissue culture to replace collagen for biomaterial purposes. Recent technological advancements such as additive manufacturing, rapid prototyping, and three-dimensional printing, in general, have resulted in great strides toward the generation of functional gelatin-based materials for medical purposes. In this review, the structural and molecular similarities of gelatin to other extracellular matrix proteins are compared and analyzed. Current strategies for gelatin crosslinking and production are described and recent applications of gelatin-based biomaterials in cell culture and tissue regeneration are discussed. Finally, recent improvements in gelatin-based biomaterials for medical applications and future directions are elaborated.

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Section: Gelatin-based 3d Microenvironment For Cell Culturementioning

confidence: 99%

Section: Fabrication Of Gelatin Nano and Microparticlesmentioning

confidence: 99%

Engineering and Functionalization of Gelatin Biomaterials: From Cell Culture to Medical Applications

Bello

Kim

et al. 2020

Tissue Engineering Part B: Reviews

Self Cite

399

259

View full text Add to dashboard Cite

show abstract

“…However, particles within spheroids are capable of controlling conditions inside the spheroids during culturing. Consequently, the viability and proliferation of cells improve (Table 4) [80,81].…”

Section: Particlesmentioning

confidence: 99%

Spheroid Culture System Methods and Applications for Mesenchymal Stem Cells

Ryu

Lee

Park

2019

Cells

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356

270

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Owing to the importance of stem cell culture systems in clinical applications, researchers have extensively studied them to optimize the culture conditions and increase efficiency of cell culture. A spheroid culture system provides a similar physicochemical environment in vivo by facilitating cell-cell and cell-matrix interaction to overcome the limitations of traditional monolayer cell culture. In suspension culture, aggregates of adjacent cells form a spheroid shape having wide utility in tumor and cancer research, therapeutic transplantation, drug screening, and clinical study, as well as organic culture. There are various spheroid culture methods such as hanging drop, gel embedding, magnetic levitation, and spinner culture. Lately, efforts are being made to apply the spheroid culture system to the study of drug delivery platforms and co-cultures, and to regulate differentiation and pluripotency. To study spheroid cell culture, various kinds of biomaterials are used as building forms of hydrogel, film, particle, and bead, depending upon the requirement. However, spheroid cell culture system has limitations such as hypoxia and necrosis in the spheroid core. In addition, studies should focus on methods to dissociate cells from spheroid into single cells.

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“…[108] Another study used cells that internalized iron oxide nanoparticles to effectively fabricate human MSC spheroids, and it was confirmed that an in vitro stem cell niche has been successfully mimicked by collagen gels embedded with magnetized spheroids. [109] An alternative to the [108] Iron oxide Human MSC [ 20,109] Polymeric micro-/nanosphere PLGA Human MSC [19,21] Gelatin Rat MSC, human ASC, human MSC [22,120,121,122] PDMS Human MSC [23] Collagen Rat hepatocytes [123] Polyacrylamide Human ASC [124] PCL Human MSC, human dermal fibroblasts [125] Hydrogel Matrigel, collagen, gelatin HepG2 [127] PNIPAAm-PEG Human PSC [128] Chitosan-PEG-genipin Glioblastoma cells [129] PEG U87, primary HDF, PC-12, INS-1, U251, human MSC [ 24,25,130] Alginate HepG2, mouse ESC, human ASC, HeLa cell [ 131,132,134,135] Heparin Mouse ESC [133] Cell membrane modification PNIPAAm HL-60, rat heart myoblasts, HepG2 [ 17,114] Oxyamine, ketone Human neonatal dermal fibroblasts, NIH3T3, 3T3 swiss albino mouse fibroblasts, human MSC [115] DNA Human luminal cells, myoepithelial cells, epithelial cells, HUVEC, human MSC [18] Fibronectin, gelatin Pancreatic cell, human pancreatic cancer cell, HUVEC, MRC-5 [ 116,117] Hyaluronic acid Renal mesangial cells [118] Gelatin, alginate, chitosan Human breast cancer cells [119] Nanofiber PLGA Embryonic kidney cells, dermal fibroblasts [ 136,137] PLLA Dermal fibroblasts, human MSC…”

Section: Use Of Magnetic Particlesmentioning

confidence: 99%

“…[22] Oxygen and nutrient diffusion were significantly improved, resulting in viability enhancement and increased proliferation, and it was confirmed that alkaline phosphatase production of stem cells within the composite spheroids was increased; similar phenomena were also observed in an ASC spheroid. [120] Gelatin microspheres have also been used as a drug carrier, and PSC spheroids formed with BMP4-laden gelatin microspheres demonstrated increased mesoderm and ectoderm lineage gene expression ( Figure 2d). [121] Microparticles and nanoparticles were also utilized as mechano-regulators inside the spheroid to modulate the mechanical properties of the cell microenvironment and to control overall spheroid stiffness for modulation of cell functions.…”

Section: Use Of Microparticles and Nanoparticlesmentioning

confidence: 99%

Engineering Multi‐Cellular Spheroids for Tissue Engineering and Regenerative Medicine

Kim

Yamamoto

et al. 2020

Adv Healthcare Materials

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148

104

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Multi-cellular spheroids are formed as a 3D structure with dense cell-cell/cell-extracellular matrix interactions, and thus, have been widely utilized as implantable therapeutics and various ex vivo tissue models in tissue engineering. In principle, spheroid culture methods maximize cell-cell cohesion and induce spontaneous cellular assembly while minimizing cellular interactions with substrates by using physical forces such as gravitational or centrifugal forces, protein-repellant biomaterials, and micro-structured surfaces. In addition, biofunctional materials including magnetic nanoparticles, polymer microspheres, and nanofiber particles are combined with cells to harvest composite spheroids, to accelerate spheroid formation, to increase the mechanical properties and viability of spheroids, and to direct differentiation of stem cells into desirable cell types. Biocompatible hydrogels are developed to produce microgels for the fabrication of size-controlled spheroids with high efficiency. Recently, spheroids have been further engineered to fabricate structurally and functionally reliable in vitro artificial 3D tissues of the desired shape with enhanced specific biological functions. This paper reviews the overall characteristics of spheroids and general/advanced spheroid culture techniques. Significant roles of functional biomaterials in advanced spheroid engineering with emphasis on the use of spheroids in the reconstruction of artificial 3D tissue for tissue engineering are also thoroughly discussed.

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Incorporation of gelatin microparticles on the formation of adipose-derived stem cell spheroids

Cited by 24 publications

References 37 publications

Engineering and Functionalization of Gelatin Biomaterials: From Cell Culture to Medical Applications

Engineering and Functionalization of Gelatin Biomaterials: From Cell Culture to Medical Applications

Spheroid Culture System Methods and Applications for Mesenchymal Stem Cells

Engineering Multi‐Cellular Spheroids for Tissue Engineering and Regenerative Medicine

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