Incorporation of Lipid-Tagged Single-Chain Antibodies into Lipid Monolayers and the Interaction with Antigen

Vikholm, Inger; Györvary, Erika; Peltonen, Jouko

doi:10.1021/la950949c

Cited by 21 publications

(14 citation statements)

References 34 publications

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“…Phage display for bio-detection is a nascent technology suitable for real-time and inexpensive field detection [5]. Antibodies can be immobilized on solid substrates via their F c fraction leaving the F ab portion free for detection using physical adsorption [21], Langmuir-Blodgett (LB) technique [22,23], and covalent coupling [24,25]. Immunosensors have serious limitations including the non-antigenic nature of the analyte, incompatibility with the sample matrix or extraction process, and the time-and labor-intensive process of making the antibodies.…”

Section: Introductionmentioning

confidence: 99%

Recognition of Salmonella typhimurium by immobilized phage P22 monolayers

et al. 2008

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Phages are promising alternatives to antibodies as the biorecognition element in a variety of biosensing applications. In this study, a monolayer of bacteriophage P22 whose tailspike proteins specifically recognize Salmonella serotypes was covalently bound to glass substrates through a bifunctional cross linker 3-aminopropyltrimethoxysilane. The specific binding of Salmonella typhimurium to the phage monolayer was studied by enzyme-linked immunosorbent assay and atomic force microscopy. Escherichia coli and a Gram-positive bacterium Listeria monocytogenes were also studied as control bacteria. The P22 particles show strong binding affinity to Salmonella typhimurium. In addition, the dried P22 monolayer maintained 50% binding capacity to Salmonella typhimurium after a one-week storage time. This is a promising method to prepare phage monolayer coatings on surface plasmon resonance and acoustic biosensor substrates in order to utilize the nascent phage display technology.

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Section: Introductionmentioning

confidence: 99%

Recognition of Salmonella typhimurium by immobilized phage P22 monolayers

et al. 2008

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“…The Langmuir−Blodgett (LB) technique allows the transfer of a monolayer from the air−water interface onto a solid support and thus enables the preparation of novel organic assemblies . Several groups have used the LB technique for biosensor design because the orientation and surface density of antibodies can be controlled. , Different approaches and molecules have been used to biofunctionalize a lipid monolayer, such as a biotinylated amphiphile−streptavidin system, protein A, N , N -dioctadecyl- N -methyl-N−(2-mercaptoethyl) ammonium bromide (DOMA), lipid-tagged single-chain antibodies, or linker lipids . Linker lipids were first used in liposomes to make the liposomes suitable for targeted drug delivery. , Egger et al , produced planar supported lipid matrixes with incorporated linker lipids by the vesicle-spreading method.…”

Section: Introductionmentioning

confidence: 99%

Miscibility in Binary Monolayers of Phospholipids and Linker Lipid

1999

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We studied the miscibility in binary lipid matrixes made by the Langmuir−Blodgett (LB) technique. The components in the lipid matrix were N-(ε-maleimidocaproyl)-dipalmitoyl phosphatidylethanolamine (DPPE-EMC; biofunctionalized linker lipid) and a phospholipid. Three different matrix phospholipids were used: 1,2-dipalmitoyl-sn-glycero-3-phosphatidylethanolamine (DPPE), 1,2-dimyristoyl-sn-glycero-3-phosphatidylethanolamine (DMPE), and 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC). The phase-transition temperature of the linker lipid as determined by Fourier transform infrared spectroscopy was 45 °C. The surface potential of the linker lipid, 290 mV at pH 6.8, was clearly smaller than the values observed for pure phospholipids. Clear evidence of the miscibility could not be obtained from the surface pressure−area isotherms. On the contrary, Brewster angle microscopy (BAM) enabled a visual investigation of the miscibility and domain morphology. The best miscibility was obtained for DPPC/DPPE-EMC matrixes but only to some extent for DPPE/DPPE-EMC and DMPE/DPPE-EMC matrixes. Atomic force microscopy on solid supported LB films showed domains similar to the BAM images of Langmuir monolayers.

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“…These conjugated IgGs can then be directly used to prepare LB films for immunosensing. 103 The pH of subphase plays a key role for electrostatic complexation of an antibody with the template LM. For example, BA has pK a of around 5.4, thus it is nearly neutrally charged at pH 5.5.…”

Section: Lb Film Based Immunosensorsmentioning

confidence: 99%

Fundamentals and application of ordered molecular assemblies to affinity biosensing

et al. 2012

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Organization of biomolecules in two/three dimensional assemblies has recently aroused much interest in nanobiotechnology. In this context, the development of techniques for controlling spatial arrangement and orientation of the desired molecules to generate highly-ordered nanostructures in the form of a mono/multi layer is considered highly significant. The studies of monolayer films to date have focused on three distinct methods of preparation: (i) the Langmuir-Blodgett (LB) technique, involving the transfer of a monolayer assembled at the gas-liquid interface; (ii) self-assembly at the liquid-solid interface, based on spontaneous adsorption of desired molecules from a solution directly onto a solid surface; and (iii) Layer-by-layer (LBL) self-assembly at a liquid-solid interface, based on inter-layer electrostatic attractions for fabrication of multilayers. A variety of monolayers have been utilized to fabricate biomolecular electronic devices including biosensors. The composition of a monolayer based matrix has been found to influence the activity(ies) of biomolecule(s). We present comprehensive and critical analysis of ordered molecular assemblies formed by LB and self-assembly with potential applications to affinity biosensing. This critical review on fundamentals and application of ordered molecular assemblies to affinity biosensing is likely to benefit researchers working in this as well as related fields of research (401 references).

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Incorporation of Lipid-Tagged Single-Chain Antibodies into Lipid Monolayers and the Interaction with Antigen

Cited by 21 publications

References 34 publications

Recognition of Salmonella typhimurium by immobilized phage P22 monolayers

Recognition of Salmonella typhimurium by immobilized phage P22 monolayers

Miscibility in Binary Monolayers of Phospholipids and Linker Lipid

Fundamentals and application of ordered molecular assemblies to affinity biosensing

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