Recognition of Salmonella typhimurium by immobilized phage P22 monolayers

Handa, Hitesh; Gurczynski, Stephen J.; Jackson, Matthew P.; Auner, Gregory W.; Walker, Jeremy; Mao, Guangzhao

doi:10.1016/j.susc.2008.01.036

Cited by 81 publications

(71 citation statements)

References 50 publications

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“…The difference between vertical and lateral sizes can be caused by adsorption, drying, and tip convolution. [50] This image is consistent with previously published data [51] it however has features, which is similar to those observed on the PEG substrate, making the discrimination of features related to either types of surface difficult. There also exist some larger features on the modified surface as the one in red circle in Figure 3b, which has a dimension of 60.5 nm × 5.5 nm (lateral diameter and height, respectively).…”

Section: Resultssupporting

confidence: 91%

Multifunctional Dendrimer‐Templated Antibody Presentation on Biosensor Surfaces for Improved Biomarker Detection

Han

Kannan

Wang

et al. 2010

Adv Funct Materials

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Dendrimers, with their well-defined globular shape and a high density of functional groups, are ideal nanoscale materials for templating sensor surfaces. This work exploits dendrimers as a versatile platform for capturing biomarkers with improved sensitivity and specificity. Synthesis, characterization, fabrication, and functional validation of the dendrimer-based assay platform are described. Bifunctional hydroxyl/thiol functionalized G4-polyamidoamine (PAMAM) dendrimer is synthesized and immobilized on to the polyethylene-glycol (PEG)-functionalized assay plate by coupling PEG-maleimide and dendrimer thiol groups. Simultaneously, part of the dendrimer thiol groups are converted to hydrazide functionalities. The resulting dendrimer-modified surface is coupled to the capture antibody in the Fc region of the oxidized antibody. This preserves the orientation flexibility of the antigen binding region (Fv) of the antibody. To validate the approach, the fabricated plates are further used as a solid phase for developing a sandwich type ELISA to detect IL-6 and IL-1β, important biomarkers for early stages of chorioamnionitis. The dendrimer-modified plate provides assays with significantly enhanced sensitivity, lower nonspecific adsorption, and a detection limit of 0.13 pg ml-1 for IL-6 luminol detection and 1.15 pg ml-1 for IL-1β TMB detection, which are significantly better than those for the traditional ELISA. The assays were validated in human serum samples from normal (non-pregnant) woman and pregnant women with pyelonephritis. The specificity and the improved sensitivity of the dendrimer-based capture strategy could have significant implications for the detection of a wide range of cytokines and biomarkers since the capture strategy could be applied to multiplex microbead assays, conductometric immunosensors and field effect biosensors.

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Section: Resultssupporting

confidence: 91%

Multifunctional Dendrimer‐Templated Antibody Presentation on Biosensor Surfaces for Improved Biomarker Detection

Han

Kannan

Wang

et al. 2010

Adv Funct Materials

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“…This two-step method was further improved by application of dithiobis(succinimidyl propionate) (DTSP) self-assembled monolayer (SAM) where the thiol group binds to the gold surface while the free succinimidyl interacts with the surface amine groups on the phages [28]. Silane chemistry has similarly been applied for silicon based substrates to facilitate P22 phage immobilization for Salmonella capture [29] as well as study of phage receptor-host ligand binding strength using atomic force microscopy [30]. In yet another example, electrochemical oxidation was used to generation of carboxyl group on carbon surface followed by amide coupling of T4 phages for subsequent E. coli capture [31].…”

Section: Recognition Elementsmentioning

confidence: 99%

Recent Advances in Bacteriophage Based Biosensors for Food-Borne Pathogen Detection

Singh

Poshtiban

Evoy

2013

Sensors

310

196

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Foodborne diseases are a major health concern that can have severe impact on society and can add tremendous financial burden to our health care systems. Rapid early detection of food contamination is therefore relevant for the containment of food-borne pathogens. Conventional pathogen detection methods, such as microbiological and biochemical identification are time-consuming and laborious, while immunological or nucleic acid-based techniques require extensive sample preparation and are not amenable to miniaturization for on-site detection. Biosensors have shown tremendous promise to overcome these limitations and are being aggressively studied to provide rapid, reliable and sensitive detection platforms for such applications. Novel biological recognition elements are studied to improve the selectivity and facilitate integration on the transduction platform for sensitive detection. Bacteriophages are one such unique biological entity that show excellent host selectivity and have been actively used as recognition probes for pathogen detection. This review summarizes the extensive literature search on the application of bacteriophages (and recently their receptor binding proteins) as probes for sensitive and selective detection of foodborne pathogens, and critically outlines their advantages and disadvantages over other recognition elements.

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“…The potential use of a phage-based biosorbent to detect, concentrate, and identify target bacteria has been reported in several studies (10,42). In one approach, physical adsorption has been used recently to immobilize filamentous and Podoviridae phages on gold surfaces of surface plasmon resonance (SPR) sensors and glass substrates to be used as a recognition element for many targeted pathogens (2,13,26). In an earlier study, Salmonella cells were captured from food matrices using Salmonella-specific phage passively immobilized on polystyrene, but this resulted in a low capture efficiency (3).…”

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confidence: 99%

Biocontrol of Listeria monocytogenes and Escherichia coli O157:H7 in Meat by Using Phages Immobilized on Modified Cellulose Membranes

Anany

Chen

Pelton

et al. 2011

Appl Environ Microbiol

204

140

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The ability of phages to specifically interact with and lyse their host bacteria makes them ideal antibacterial agents. The range of applications of bacteriophage can be extended by their immobilization on inert surfaces. A novel method for the oriented immobilization of bacteriophage has been developed. The method was based on charge differences between the bacteriophage head, which exhibits an overall net negative charge, and the tail fibers, which possess an overall net positive charge. Hence, the head would be more likely to attach to positively charged surfaces, leaving the tails free to capture and lyse bacteria. Cellulose membranes modified so that they had a positive surface charge were used as the support for phage immobilization. It was established that the number of infective phages immobilized on the positively charged cellulose membranes was significantly higher than that on unmodified membranes. Cocktails of phages active against Listeria or Escherichia coli immobilized on these membranes were shown to effectively control the growth of L. monocytogenes and E. coli O157:H7 in ready-to-eat and raw meat, respectively, under different storage temperatures and packaging conditions. The phage storage stability was investigated to further extend their industrial applications. It was shown that lyophilization can be used as a phage-drying method to maintain their infectivity on the newly developed bioactive materials. In conclusion, utilizing the charge difference between phage heads and tails provided a simple technique for oriented immobilization applicable to a wide range of phages and allowed the retention of infectivity.

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Recognition of Salmonella typhimurium by immobilized phage P22 monolayers

Cited by 81 publications

References 50 publications

Multifunctional Dendrimer‐Templated Antibody Presentation on Biosensor Surfaces for Improved Biomarker Detection

Multifunctional Dendrimer‐Templated Antibody Presentation on Biosensor Surfaces for Improved Biomarker Detection

Recent Advances in Bacteriophage Based Biosensors for Food-Borne Pathogen Detection

Biocontrol of Listeria monocytogenes and Escherichia coli O157:H7 in Meat by Using Phages Immobilized on Modified Cellulose Membranes

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