Incorporation of microencapsulated Lactobacillus rhamnosus into infant-foods inhibit proliferation of toxicogenic Bacillus cereus strains

Abdel-Rahman, Mohamed Ali; Sadek, Zeinab I.; Azab, Mohamed Salah; Darwesh, Osama M.; Hassan, Mahmoud S.

doi:10.1016/j.bcab.2019.01.051

Cited by 7 publications

(6 citation statements)

References 28 publications

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“…This gene is the most important one in pyocyanin production because it is considered the final gene in biosynthesis pathway and responsible for hydroxylation and decarboxylation of 5-methylphenazine 1-carboxylic acid betaine to pyocyanin. These clearly show the P. aeruginosa strain Osh1 contains phenazine biosynthetic operon and modifying genes which response to produce pyocyanin pigment [13]. Sequentially, the proteomic studies of pyocyanin biosynthesis modified genes were done on P. aeruginosa strain to confirm their having of the studied genes.…”

Section: Discussionmentioning

confidence: 91%

“…The pure isolates were confirmed on cetrimide medium. Then the pure colonies were preserved on cetrimide agar slants at 4°C [13]. The primary identification of isolates was done based on procedures detailed in Bergey's Manual of Systematic Bacteriology [14].…”

Section: Methodsmentioning

confidence: 99%

“…The DNA purity and concentration were determined according to Barbas et al [26] by measuring the absorbance at λ 260nm and λ 280nm using UV/VIS spectrophotometer (Jenway UV/Visible-2605 spectrophotometer, England) and confirmed on 1% agarose gel electrophoresis [27]. To detect the phenazine -modifying gene (phzS) involved in biosynthesis of pyocyanin in P. aeruginosa strain OSh1, the PCR amplification was conducted in 50 µL containing 2.5U of Dream-Taq DNA polymerase (Thermo scientific, formerly Fermentas, Lithuania), 1x Dream-Taq Buffer, 40 ng of extracted DNA, 0.2 mM of dNTPs, 1 mM MgCl 2 and 20 pmol of forward oligonucleotide primer (5'-TGCGCTACATCGACCAGAG-3') and the reverse primer (5'-CGGGTACTGCAGGATCAACT-3') [13]. The PCR reaction was performed with thermal cycler (MJ Research PTC-100, MJ Research Inc., MA, USA) by amplification conditions started with pre-denaturation step of double stranded DNA at 94°C for 5 min; followed 35 cycles of denaturation step 94°C for 40 sec, annealing step 48°C for 40 sec, extension step 72°C for 1 min; then one step of final extension 72°C for 10 min, and storage at 4°C.…”

Section: Molecular Detection Of Phenazine Modifying Gene (Phzs)mentioning

confidence: 99%

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Production of antimicrobial blue green pigment Pyocyanin by marine Pseudomonas aeruginosa

2019

Biointerface Res Appl Chem

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This study focused on isolation and molecular identification of marine bacteria which producing bioactive pigment. For that, 10 pigmented bacteria were isolated from 3 water samples collected from Mediterranean Sea, Alex., Egypt. One isolate was had efficient value to produce blue green pigment. This one was identified using molecular biology techniques and recorded in GeneBank under title of Pseudomonas aeruginosa strain OSh1 with accession number KT032066. The produced pigment was extracted, purified and characterized as pyocyanin. The pathway of pyocyanin production contains many sequential genes, the important one is phzS. This gene was detected genetically in this study using specific primers and gave PCR product with 448 bp. To confirm that, the inducible proteins for pyocyanin synthesis genes were documented using SDS-PAGE. The results represent found 2 proteins with 36 and 43 kDa for phzM and phzS, respectively. The biological activity of the produced pyocyanin at different concentrations was investigated as antimicrobial agent. The antibacterial activity was ranged between 31- 42 mm of inhibition zone. Whereas, the inhibition zone for antifungal activity was ranged between 34- 35 mm. The results indicate the produced pyocyanin had high activity against multi-drug resistant microbes and we can use it as marine antibiotic.

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Section: Discussionmentioning

confidence: 91%

Section: Methodsmentioning

confidence: 99%

Section: Molecular Detection Of Phenazine Modifying Gene (Phzs)mentioning

confidence: 99%

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Production of antimicrobial blue green pigment Pyocyanin by marine Pseudomonas aeruginosa

2019

Biointerface Res Appl Chem

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“…7). Another important factor for actinomyces growth or enzymes and other bioactive compounds production is the initial pH value of cultivation media [78]. In this way, Thf.…”

Section: Optimization Of Beneficial Enzymes Productionmentioning

confidence: 99%

Thermoflavimicrobium dichotomicum as a novel thermoalkaliphile for production of environmental and industrial enzymes

2019

Biointerface Res Appl Chem

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Thermoalkaliphilic actinomycetes enzymes have many important applications in many industrial, biotechnological and environmental aspects. So, the current study aimed to obtain the thermoalkali-enzymes producing actinomycetes. A novel thermoalkaliphilic actinomycete strain was isolated from Egyptian Siwa oasis and identified according to its morphological, physiological and biochemical characters as Thermoflavimicrobium dichotomicum. And then confirmed by phylogenetic analysis and the partial sequence was deposited in GenBank under accession number of KR011193 and name of Thermoflavimicrobium dichotomicum HwSw11. It could produce amylase, cellulase, lipase, pectinase and proteinase enzymes. Also, this strain exhibited anti-bacterial activities against P. aeruginosa and E. coli with inhibition zones of 14 and 20 mm, respectively. Consequently, it has antifungal activity against A. niger, A. flavus and Penicillium notatum with inhibition zones of 17, 14 and 14 mm, respectively. For that, it may be concluded that Thermoflavimicrobium dichotomicum HwSw11 as a novel thermoalkaliphile has a wide range of biological activities against a broad spectrum of pathogenic bacteria and fungi, in addition to produce many enzymes (amylase, cellulase, lipase, pectinase and proteinase). So, this isolate could be applied as manufactory for many industrial, biotechnological and environmental sectors.

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“…Propionic acid is a volatile fatty acid that is produced from microbes existed in the human and animal gut. Propionic acid and propionates have significant inhibiting effects on many fungi and yeasts as well as bacterial strains [25][26][27][28]. The effect of these compounds on fungi is inhibitory (fungistatic) rather than fatal (fungicidal).…”

Section: Chemicals As Food Preservativesmentioning

confidence: 99%

Food Preservation: Comprehensive overview of techniques, applications and hazards

et al. 2022

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Without food preservation, human life is in danger. Because there are many serious diseases transmitted through spoiled foods called foodborne diseases, like meningitis, diarrhea and cancer, and can be lead to long-lasting disability and death. Therefore, there had to be many ways to preserve food to prevent poisoning, illnesses, increase the shelf life of foods and maintain its nutritional values. Food preservation is a very sensitive issue and its' techniques can generally be divided into three categories: physical, chemical and biological. This paper aims to review the three main categories of food preservation methods. Definitions, types, applications, advantages and disadvantages of each category will be considered with emphasis on its' importance to the future of the food industry, antimicrobial activities and consumers' health. Chemical-based preservation methods' hazards will be discussed and possible side effects and diseases were mentioned. Biological preservation methods have been extensively reviewed with explanations of the natural antimicrobials from animal, microbial and plant origins. This review paper lists and discusses the three types of food preservation methods and focuses on, natural methods from different origins to maintain the quality and nutritional values of foods as well as extend their shelf life.

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Incorporation of microencapsulated Lactobacillus rhamnosus into infant-foods inhibit proliferation of toxicogenic Bacillus cereus strains

Cited by 7 publications

References 28 publications

Production of antimicrobial blue green pigment Pyocyanin by marine Pseudomonas aeruginosa

Production of antimicrobial blue green pigment Pyocyanin by marine Pseudomonas aeruginosa

Thermoflavimicrobium dichotomicum as a novel thermoalkaliphile for production of environmental and industrial enzymes

Food Preservation: Comprehensive overview of techniques, applications and hazards

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