Incorporation of Sulphur-35 into the Subcommissural Organ and Reissner's Fibre

The subcommissural organ

“…Olsson (1958a) indicated that the secretion of the bovine SCO must be rich in sialic acid, perhaps in the form of a syalilated protein. Autoradiographic investigations after the administration of radioactive cisteine and cystine have shown that the SCO and the RF become strongly labeled (Diederen et al, 1987;Ermisch, 1973;Leatherland and Dodd, 1968;Rodríguez et al, 1992;Sterba et al, 1967b). In brief, all the histochemical studies have indicated that the secretory material of the SCO is a carbohydrate-protein complex (or a complex of glycoproteins) with a high content of cysteine and sialic acid.…”

“…20). Thus, 2 hours after the administration of radioactive cysteine, the label is found in the proximal portion of RF; as the postinjection time increases, the label is found in more distal segments of RF (Sterba et al, 1967b). This experimental approach has allowed us to determine the growth rate of RF.…”

“…This experimental approach has allowed us to determine the growth rate of RF. In the mouse, RF grows 10% of its length per day (Ermisch, 1973); in the rat, 7% per day (Herrera, 1988); and in the carp and lamprey, about 1% per day (Ermisch et al, 1968;Sterba et al, 1967b).…”

Biosynthesis and molecular biology of the secretory proteins of the subcommissural organ

“…1). RF grows caudally and extends along the brain aqueduct, the fourth ventricle, and the whole length of the central canal of the spinal cord Oksche, 1969;Olsson, 1993;Pérez et al, 1996;Rodríguez et al, 1985Rodríguez et al, , 1998Sterba et al, 1967;Wolf and Sterba, 1972).…”

Functional aspects of the subcommissural organ-Reissner's fiber complex with emphasis in the clearance of brain monoamines

“…The Pre-RF-material (Rodríguez et al, , 1992 was first demonstrated at the light-microscopic level using the same staining and histochemical procedures that reveal the intracellular secretory material of the SCO (Herrera and Rodríguez, 1990;Naumann, 1968;Oksche, 1961;Olsson, 1958;Rodríguez et al, 1986Rodríguez et al, , 1987bSterba et al, 1967;Wingstrand, 1953). The layer of pre-RF material has also been visualized by transmission electron microscopy (Hofer et al, 1980;Muller and Sterba, 1965;Rodríguez, 1970;Sterba et al, 1967;Wenger et al, 1969) and scanning electron microscopy (Krstic, 1975) (Fig.…”