Incorporation of water molecules among the hydrophilic groups of film molecules by thermal treatment of monolayers on the water surface at certain high temperatures

Kato, Takeshi; Shimizu, Yasushi; Kameyama, Makoto; Kimura, Yoshiki; Okahata, Yoshio

doi:10.1016/s0040-6090(95)08263-8

Cited by 4 publications

(3 citation statements)

References 10 publications

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“…A π-A isotherm of the thermally hydrated DPPC Langmuir film was measured to check the film-expansion effect. 8 To prepare the hydrated L film, 70 µL of the DPPC solution in chloroform of 1 mg mL -1 was dropped on a subphase solution (pH 7.4) at 25 °C, and it was left for 10 min to evaporate the solvent. This temperature is below the main-transition temperature (circa 41 °C) of DPPC.…”

Section: Resultsmentioning

confidence: 99%

“…Kato et al most recently reported 8 that a thermally hydrated Langmuir (spread monolayer) film of DMPA (dimirystoylphosphatidic acid) on a water surface was dramatically expanded by the incorporated water molecules in the Langmuir (L) film. The thermal treatment was typically done by raising the temperature once, and the heated L film was cooled down again to the original temperature.…”

Section: Introductionmentioning

confidence: 99%

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Thermally Hydrated DPPC Langmuir Film: A Trial Application to the Analysis of Interaction of Sucrose with DPPC Liposome

1997

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The thermally hydrated dipalmitoyl phosphatidyl choline (DPPC) Langmuir (L) film was investigated to determine whether it could be an appropriate physical model of DPPC liposomes. The interaction of sucrose with the L film and the liposome was investigated for the examination. The conventional DPPC L film without any heating process showed a film shrinkage by bound sucrose on the surface of the L film. The film shrinkage was enhanced with an increase of the concentration of sucrose. This shrinkage was found to affect all of the entire characteristics of the conventional L film. On the other hand, the hydrated L film by a heating process indicated a film expansion by the penetrated sucrose, although the incorporated water in the film avoided the approach of sucrose a little. The expansion increased with the concentration of sucrose, a fact that was contrary to the results of the conventional L film. To compare the hydrated L film with liposome, the fluorescence decay and anisotropy of 1,6-diphenyl-1,3,5-hexatriene (DPH) in DPPC liposomes were measured. The results indicated that the molecular order of DPPC in the liposome decreased with the concentration of sucrose. The molecular motion of DPPC was reduced with the concentration of sucrose. These phenomena were successfully explained by the results of the hydrated L films. The hydrated L film was consequently proposed to be a much better physical model of liposomes than the conventional L film.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Thermally Hydrated DPPC Langmuir Film: A Trial Application to the Analysis of Interaction of Sucrose with DPPC Liposome

1997

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“…In the line of these studies, we previously reported the availability of thermally fully hydrated , Langmuir (L) films (spread monolayers on the aqueous solution) for the investigation of molecular interaction between the phospholipid membrane and sucrose. The fully hydrated DPPC L films were prepared by a heating process via a temperature beyond the transition temperature (41 °C) 7 of DPPC L film.…”

Section: Introductionmentioning

confidence: 99%

Binding Mechanism of Sucrose to Dipalmitoylphosphatidylcholine Langmuir Films by Fourier Transform Infrared Reflection−Absorption Spectroscopy and Quartz-Crystal Microbalance Technique

1998

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Quartz-crystal microbalance (QCM) and Fourier transform infrared (FTIR) reflection−absorption (RA) techniques are employed to investigate the binding mechanism of fully hydrated (thermally treated) DPPC Langmuir (L) films with sucrose in the aqueous solution. Sucrose molecules incorporated in the fully hydrated L film strongly bind to the L film even when the L film is laterally compressed to a high surface pressure, while those in partially hydrated (without thermal treatment) L films are squeezed out by the compression. When the L film is compressed to a low surface pressure corresponding to the liquid-crystalline (soft) state, the incorporated sucrose molecules remain well in both fully and partially hydrated L films. The amount of bound sucrose in the fully hydrated soft L film linearly increases with the concentration of sucrose. This indicates that the fully hydrated soft DPPC L film forms a mingled layer where a hydrated DPPC film and deeply incorporated sucrose molecules are inseparable from each other. In other words, incorporated sucrose molecules in the surface of the fully hydrated soft DPPC L films are very stable.

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Modern physicochemical research on Langmuir monolayers

Dynarowicz-Łątka

Dhanabalan

Oliveira

2001

Advances in Colloid and Interface Science

329

244

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Incorporation of water molecules among the hydrophilic groups of film molecules by thermal treatment of monolayers on the water surface at certain high temperatures

Cited by 4 publications

References 10 publications

Thermally Hydrated DPPC Langmuir Film: A Trial Application to the Analysis of Interaction of Sucrose with DPPC Liposome

Thermally Hydrated DPPC Langmuir Film: A Trial Application to the Analysis of Interaction of Sucrose with DPPC Liposome

Binding Mechanism of Sucrose to Dipalmitoylphosphatidylcholine Langmuir Films by Fourier Transform Infrared Reflection−Absorption Spectroscopy and Quartz-Crystal Microbalance Technique

Modern physicochemical research on Langmuir monolayers

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