Increase effected by calcium ion in the rate of oxygen evolution from preparations of Phormidium luridum

Piccioni, Richard G.; Mauzerall, D.

doi:10.1016/0005-2728(76)90213-9

Cited by 40 publications

(8 citation statements)

References 10 publications

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“…dium (25). While both Mg2e and Ca2+ stimulated turnover rates of PSII reaction centers in Phormidium membranes, Ca2" alone stabilized a maximum number of active reaction centers (26).…”

mentioning

confidence: 95%

Anacystis nidulans Demonstrates a Photosystem II Cation Requirement Satisfied Only by Ca²⁺ or Na⁺

Becker¹,

Brand²

1985

Plant Physiol.

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ABSTRACITAnacystis nidulans exhibits a total loss of photosystem II (PSII) activity upon incubation in a nutrient medium deficient in Ca2' and Na' and containing a divalent cation chelator. This loss of activity is lightdependent, which corresponds to an energy requirement. Likewise, Ca2' efflux takes place only in cells incubated in light. The loss of PSII activity is reversible by addition of submillimolar amounts of either Ca2' or Na' to the external medium but not by the addition of any other cation. Restoration of lost PSII activity also requires light. Light saturation curves for partially depleted cells demonstrate both lower maximum 02 evolution rates and decreased relative quantum yields when compared to control cells. Partial electron transport reactions isolate the site of the Ca2'/Na' effect to the reaction center itself or immediately on its oxidizing side and exclude the water-splitting complex. 02 flash yields decline during cation depletion, indicating a decrease in the number of functional PSII reaction centers, but the maximum turnover rate for still functional reaction centers does not decline. Thus, PSII of A. nidulans exhibits an all-or-none cation requirement, satisfied only by Ca2 or Na'.Early preparations of photosynthetic membranes from cyanobacteria required divalent cations for maximal activity (12,31 (7). We report here a full description of conditions necessary for the reversible loss of PSII activity in intact Anacystis, including the startling finding that Na+ substitutes for Ca2" in this system. We also demonstrate that the site of action for these cations is very near the reaction center. A summary of portions of this work has been presented at a symposium (4). MATERIALS AND METHODSCell Growth and Incubation Conditions. Cg-10 medium was prepared in 3-L batches from stock solutions as described in (32) except that the Fe-EDTA stock solution was prepared from the free acid form of EDTA and the final glycylycine concentration was 11 mm. Acid-washed (0.2 N HCI) glassware was used throughout to minimize cation contamination and the medium was sterilized by autoclaving prior to use. The pH was adjusted to 8.3 with KOH.Anacystis (UTEX 625, Collection of Algae, University of Texas, Austin) was grown at 39C in continuous culture in nutrient medium (28). Cell suspensions of approximately 2 x 108 cells ml-' were aerated continuously with 1.2% CO2 in air and illuminated at approximately 50 uE m-2 s-' PAR with F20T12-CW fluorescent lamps. Cells were harvested, pelleted at 2000g for 10 min, then resuspended to the original cell density in Cg-10 medium devoid of Ca2+. The suspension was pelleted a second time and resuspended in a small volume ofCa2`deficient

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“…dium (25). While both Mg2e and Ca2+ stimulated turnover rates of PSII reaction centers in Phormidium membranes, Ca2" alone stabilized a maximum number of active reaction centers (26).…”

mentioning

confidence: 95%

Anacystis nidulans Demonstrates a Photosystem II Cation Requirement Satisfied Only by Ca²⁺ or Na⁺

Becker¹,

Brand²

1985

Plant Physiol.

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“…Indeed, it was found that in special cell free preparations of Phormiclium luridum a DCMU resistant oxygen evolution can be obtained with K3 [Fe(CN)6] as the electron acceptor [9,10]. Furthermore, electron acceptors like silicomolybdate which are also effective as structural modifiers [ 11 ] were shown to mediate a system II electron transport insensitive to DCMU [ 12].…”

Section: Introductionmentioning

confidence: 99%

The induction of a high resistance to 3‐(3,4‐dichlorophenyl‐1,1‐dimethylurea (DCMU) of oxygen evolution in spinach chloroplasts by trypsin treatment

Ренгер¹

1976

FEBS Letters

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“…Calcium has been suggested to play an important role in cyanobacterial photosynthesis (Piccioni & Mauzerall 1976). Debus (1992) showed that the influence of Ca 2 ' ions on electron transfer and oxygen evolution might be different between cyanobacteria and higher plants.…”

Section: Discussionmentioning

confidence: 99%

Physiological and molecular responses to calcium supplementation inMicrocystis aeruginosa(Cyanobacteria)

Shi¹,

Wu²,

Song³

2013

New Zealand Journal of Marine and Freshwater Research

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Calcium is an important element in water bodies that plays a pivotal role in numerous biological processes; however, its effects on bloom-forming cyanobacteria have been studied only rarely. In the present study, the effects of calcium on Microcystis aeruginosa, a major bloom-forming cyanobacterium, were investigated. Significant decreases in growth, chlorophyll a and maximum electron transport rate were shown when calcium was absent, while similar reductions in these parameters were also found at high concentrations of calcium (240 mg/l). The expression of psbA, grpE and recA was up-regulated significantly for 0 and 240 mg/l calcium treatments. The activity of catalase (CAT) also increased remarkably under these two treatments, suggesting that Microcystis suffered from stress under both calcium absence and under high calcium concentrations. Additionally, the polysaccharide synthesis gene, epsL, was up-regulated at high concentrations of calcium. These data suggest that calcium plays an important role in the growth of Microcystis, and that enhancement in polysaccharide synthesis in response to increases in calcium concentration might be responsible for bloom formation.

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Increase effected by calcium ion in the rate of oxygen evolution from preparations of Phormidium luridum

Cited by 40 publications

References 10 publications

Anacystis nidulans Demonstrates a Photosystem II Cation Requirement Satisfied Only by Ca²⁺ or Na⁺

Anacystis nidulans Demonstrates a Photosystem II Cation Requirement Satisfied Only by Ca²⁺ or Na⁺

The induction of a high resistance to 3‐(3,4‐dichlorophenyl‐1,1‐dimethylurea (DCMU) of oxygen evolution in spinach chloroplasts by trypsin treatment

Physiological and molecular responses to calcium supplementation inMicrocystis aeruginosa(Cyanobacteria)

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Increase effected by calcium ion in the rate of oxygen evolution from preparations of Phormidium luridum

Cited by 40 publications

References 10 publications

Anacystis nidulans Demonstrates a Photosystem II Cation Requirement Satisfied Only by Ca2+ or Na+

Anacystis nidulans Demonstrates a Photosystem II Cation Requirement Satisfied Only by Ca2+ or Na+

The induction of a high resistance to 3‐(3,4‐dichlorophenyl‐1,1‐dimethylurea (DCMU) of oxygen evolution in spinach chloroplasts by trypsin treatment

Physiological and molecular responses to calcium supplementation inMicrocystis aeruginosa(Cyanobacteria)

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Anacystis nidulans Demonstrates a Photosystem II Cation Requirement Satisfied Only by Ca²⁺ or Na⁺

Anacystis nidulans Demonstrates a Photosystem II Cation Requirement Satisfied Only by Ca²⁺ or Na⁺