Increase in the relative level of type V collagen in the placentae of patients with pre-eclampsia

Iwahashi, M

doi:10.1093/molehr/3.8.725

Cited by 6 publications

(3 citation statements)

References 35 publications

(36 reference statements)

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“…COL5A1 , an extracellular matrix (ECM) protein known to be involved in connective tissue disorders, has been identified in placental stromal cells and has been shown to be increased in PE placentas [47]. Similarly, in our study, hypermethylation of COL5A1 in preterm PE placentas resulted in increased mRNA expression.…”

Section: Discussionsupporting

confidence: 75%

Differential Methylation of Genes Associated with Cell Adhesion in Preeclamptic Placentas

et al. 2014

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Preeclampsia (PE), a hypertensive disorder of pregnancy, is hypothesized to be associated with, if not mechanistically related to abnormal placental function. However, the exact mechanisms regulating the pathogenesis of PE remain unclear. While many studies have investigated changes in gene expression in the PE placenta, the role of epigenetics in PE associated placental dysfunction remains unclear. Using the genome-wide Illumina Infinium Methylation 450 BeadChip array, we analyzed gene-specific alterations in DNA methylation in placental biopsies collected from normal pregnant women delivering at term (n = 14), with term PE (≥37 weeks; n = 19) or with preterm PE (<37 weeks, n = 12). Of the 485,582 gene loci on the array, compared to controls, 229 loci were differentially methylated in PE placentas and 3411 loci were differentially methylated in preterm PE (step up p-value <0.05 and >5% methylation difference). Functional annotation of the differentially methylated genes in preterm PE placentas revealed a 32 gene cluster in the cadherin and cell adhesion functional groups (Benjamini p<0.00001). Hypermethylation of CDH11 (p = 0.0143), COL5A1 (p = 0.0127) and TNF (p = 0.0098) and hypomethylation of NCAM1 (p = 0.0158) was associated with altered mRNA expression in preterm PE placentas. Demethylation of first trimester extravillous trophoblast cells resulted in altered CDH11 (p = 0.0087), COL5A1 (p = 0.0043), NCAM1 (p = 0.0260) and TNF (p = 0.0022) mRNA expression. These studies demonstrate aberrant methylation, correlating with disease severity, in PE placentas. Furthermore, we provide evidence that disruption of gene-specific methylation in preterm PE placentas and first trimester trophoblasts is significantly associated with altered gene expression demonstrating that epigenetic modifications early in pregnancy can have effects on trophoblast function contributing to PE.

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Section: Discussionsupporting

confidence: 75%

Differential Methylation of Genes Associated with Cell Adhesion in Preeclamptic Placentas

et al. 2014

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“…This suggested a change in these ratios in the placenta during the process of development and aging [17]. In contrast, the ratio of type III to type I was lower, and that of type V to type I was higher, in placentae from preeclampsia compared with normal term placentae.…”

Section: Discussionmentioning

confidence: 97%

“…The thorough analysis of data of all available studies of collagen metabolism in preeclampsia [12][13][14][15][16][17][18] shows that the extent of the elevation of each marker was mild to moderate. Authors conclude that it is less likely that PICP or PIIINP levels, measured in the second trimester, would be predictors of preeclampsia, although this remains to be tested.…”

Section: Discussionmentioning

confidence: 99%