Increase in the Thermal Stability of Phytase from Citrobacter freundii by Site-Directed Saturation Mutagenesis

Gordeeva, T. L.; Borshchevskaya, L. N.; Kalinina, A. N.; Sineoky, S. P.; Kashirskaya, M.D.; Voronin, S. P.

doi:10.1134/s0003683819080052

Cited by 2 publications

(1 citation statement)

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“…To this end, Bedford ( 2000 ) explored the use of genetic engineering to improve exogenous enzyme thermal stability and adaptation to the high temperatures of the process of feed preparation. Gordeeva et al ( 2019 ) increased the thermal stability of enzymes used in feedstuffs by site-directed saturation mutagenesis. Genetic engineering has been widely used to improve enzyme properties to promote their better application.…”

Section: Enzyme Addition Proceduresmentioning

confidence: 99%

Application of enzymes as a feed additive in aquaculture

Liang

Yuan

et al. 2022

Mar Life Sci Technol

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Modern aquaculture must be sustainable in terms of energy consumption, raw materials used, and environmental impact, so alternatives are needed to replace fish feed with other raw materials. Enzyme use in the agri-food industry is based on their efficiency, safety, and protection of the environment, which aligns with the requirements of a resource-saving production system. Enzyme supplementation in fish feed can improve digestibility and absorption of both plant- and animal-derived ingredients, increasing the growth parameters of aquacultural animals. Herein we summarized the recent literature that reported the use of digestive enzymes (amylases, lipases, proteases, cellulases, and hemicellulases) and non-digestive enzymes (phytases, glucose oxidase, and lysozyme) in fish feed. In addition, we analyzed how critical steps of the pelleting process, including microencapsulation and immobilization, can interfere with enzyme activity in the final fish feed product.

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Section: Enzyme Addition Proceduresmentioning

confidence: 99%