2002
DOI: 10.1046/j.1523-1747.2002.01647.x
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Increase of Pro-opiomelanocortin mRNA Prior to Tyrosinase, Tyrosinase-Related Protein 1, Dopachrome Tautomerase, Pmel-17/gp100, and P-Protein mRNA in Human Skin After Ultraviolet B Irradiation

Abstract: In ultraviolet-induced tanning, the protein levels of various gene products critical for pigmentation (including tyrosinase and tyrosinase-related protein-1) are increased in response to ultraviolet B irradiation, but changes in mRNA levels of these factors have not been investigated in vivo. We have established an in situ hybridization technique to investigate mRNA levels of pro-opiomelanocortin, tyrosinase, tyrosinase-related protein-1, dopachrome tautomerase, P-protein, Pmel-17/gp100, and microphthalmia-ass… Show more

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Cited by 39 publications
(38 citation statements)
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“…This provides unequivocal evidence that POMC derivatives function as autocrine/paracrine regulators of melanocytes. Expression of POMC has been found to be further stimulated upon UV irradiation of the skin in vivo, thus lending support for a physiological role for POMC-derived peptides in regulating the response of melanocytes to UV [23,113].…”
Section: Melanocortins and The Melanocortin Receptorsmentioning
confidence: 84%
See 1 more Smart Citation
“…This provides unequivocal evidence that POMC derivatives function as autocrine/paracrine regulators of melanocytes. Expression of POMC has been found to be further stimulated upon UV irradiation of the skin in vivo, thus lending support for a physiological role for POMC-derived peptides in regulating the response of melanocytes to UV [23,113].…”
Section: Melanocortins and The Melanocortin Receptorsmentioning
confidence: 84%
“…Pro-opiomelanocortin is synthesized in human skin, mainly by keratinocytes and melanocytes in the epidermis [23,90,113]. Epidermal cells also express pro-convertases, thus can process POMC into bioactive derivatives, including a-MSH, ACTH, and b-endorphin [11,20,85,90,124,131].…”
Section: Melanocortins and The Melanocortin Receptorsmentioning
confidence: 99%
“…Tissue in-situ hybridization TISH was carried out as described previously (Suzuki et al, 2002) with minor modifications. Briefly, cross sections of paraffin-embedded skins (3 m) were deparaffinized, treated with proteinase K, and placed in 200 ml acetylation buffer (0.1 M triethylamine, pH 8.0, containing 0.25% acetic anhydride) for 15 minutes.…”
Section: Microarray Proceduresmentioning
confidence: 99%
“…Further supporting evidence of this is that both tyrosinase and TRP-1 mRNA levels have been found in vivo to be significantly increased in biopsies of normal skin five days after exposure to ultraviolet B irradiation. 10 Ultraviolet B irradiation is also known to increase the synthesis of alpha-melanocyte-stimulating hormone (alpha-MSH) and adrenocorticotropin (ACTH), two peptides derived from pro-opiomelanocortin (POMC). 11 These peptides bind to the melanocortin-1 receptor (MC-1R), inducing the formation of cyclic AMP (cAMP).…”
Section: Ultraviolet Radiationmentioning
confidence: 99%