Increase of protein extraction yield from rapeseed meal through a pretreatment with phytase

Rodrigues, Ivo; Carvalho, M. G.; Rocha, J. M. S.

doi:10.1002/jsfa.8087

Cited by 36 publications

(16 citation statements)

References 20 publications

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“…Alkaline extraction is the most commonly used method to extract proteins from oilseed meals (Rodrigues et al ., ). Isolated protein from RSM (PRSM) could not only increase the percentage of protein, but also reduce mineral elements from RSM.…”

Section: Resultsmentioning

confidence: 97%

“…Phytate is the major storage form of phosphorus in most cereal grains and oilseeds, and it usually accounts for 60–90% of the total available phosphorus. Beside its ability to chelate and precipitate minerals, phytate is also closely associated with proteins, especially in oilseeds, decreasing the bioavailability of critical nutrients such as protein, calcium, magnesium, zinc and iron (Haese et al ., ; Rodrigues et al ., ). Therefore, we predicted that the content of soluble phosphorus in the hydrolysate could reflect the hydrolysis ability of Bacillus for RSM to a great extent.…”

Section: Resultsmentioning

confidence: 97%

“…Compared with the 1.15 g l À1 iturin A obtained in the medium with added inorganic salts, the production of iturin A increased 8.70%, which indicated that the addition of inorganic salts might have a negative effect on iturin A production when RSM was used as nitrogen source. Alkaline extraction is the most commonly used method to extract proteins from oilseed meals (Rodrigues et al, 2017). Isolated protein from RSM (PRSM) could not only increase the percentage of protein, but also reduce mineral elements from RSM.…”

Section: Influence Of Initial Glucose and Rsm Concentrations On Iturimentioning

confidence: 99%

“…However, under the conditions of equal protein content, the yield of iturin A obtained from PRSM was still higher than that obtained from RSM. This phenomenon is probably caused by a combination and interaction of other components of RSM such as fibre, non-starch polysaccharides (de Vries et al, 2014) or phytate (Haese et al, 2017;Rodrigues et al, 2017). Further pretreatment, which could make the nutrients in RSM more accessible, might be an effective way to improve the RSM utilization (Chen et al, 2011;Chatzifragkou et al, 2014).…”

Section: Influence Of Initial Glucose and Rsm Concentrations On Iturimentioning

confidence: 99%

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Establishment of a rapeseed meal fermentation model for iturin A production by Bacillus amyloliquefaciens CX‐20

Chen

Wang

et al. 2019

Microbial Biotechnology

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Summary Iturin A is an important broad‐spectrum antifungal cyclic lipopeptide used as an ideal potential biological control agent. However, its application is limited mainly due to the producer strains’ low productivity and the high production costs. Here, a potentially industrial strain Bacillus amyloliquefaciens CX‐20 was proved to use low‐cost rapeseed meal (RSM) as the sole source of all nutrients except the carbon source for the high productivity of iturin A. A fermentation model was first established to analyse the specific roles of different RSM components on iturin A production. Proteins and minerals in RSM were confirmed to play positive role, whereas fibre had negative effect. And the maximal concentration of iturin A was predicted to be more than 1.64 g l−1 by the established evaluation model. Moreover, submerged fermentation of B. amyloliquefaciens CX‐20 demonstrated a strong ability to hydrolyse RSM and release water‐soluble nutrients. This fermentation broth, a mixture of Bacillus, iturin A and RSM hydrolysate, could simultaneously combat clubroot disease and promote the growth of Brassica napus. In conclusion, this study provides a promising strategy to achieve full utilization of RSM for the production of a combination of value‐added biological control agent and biofertilizer.

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Section: Resultsmentioning

confidence: 97%

Section: Resultsmentioning

confidence: 97%

Section: Influence Of Initial Glucose and Rsm Concentrations On Iturimentioning

confidence: 99%

Section: Influence Of Initial Glucose and Rsm Concentrations On Iturimentioning

confidence: 99%

See 2 more Smart Citations

Establishment of a rapeseed meal fermentation model for iturin A production by Bacillus amyloliquefaciens CX‐20

Chen

Wang

et al. 2019

Microbial Biotechnology

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“…The double mutants produced in this study could be crossed with elite cultivars to breed low phytic acid varieties. One of the major aims of oilseed rape breeding is to improve the meal quality, which is rich in favorable proteins and amino acid profiles for human and mono‐gastric animal nutrition (Campbell et al , ; Rodrigues et al , ). Hitherto, external phytases are added to the meal to decrease phytic acid contents and to increase the bioavailability of minerals (Al‐Asheh & Duvnjak, ; Campbell et al , ).…”

Section: Discussionmentioning

confidence: 99%

Identification of phytic acid mutants in oilseed rape (Brassica napus) by large‐scale screening of mutant populations through amplicon sequencing

Sashidhar

Harloff

2019

New Phytologist

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Brassica napus (oilseed rape) is an important oil crop in temperate regions, which originated from hybridization of Brassica oleracea and Brassica rapa. Owing to its polyploidy, the functional study of single genes is cumbersome. Phytic acid is considered as an antinutritive compound, and we aimed to knock out the underlying synthesis and transporter genes to identify low phytic acid mutants.We implemented a high-throughput next-generation sequencing screening protocol for an ethylmethane sulfonate population of 7680 plants in six gene families (BnMIPS, BnMIK, Bn2-PGK, BnIPK1, BnIPK2, and BnMRP5) with two paralogues for each gene.A total of 1487 mutations were revealed, and the vast majority (96%) were confirmed by Sanger sequencing. Furthermore, the characterization of double mutants of Bn.2-PGK2 showed a significant reduction of phytic acid contents.We propose to use three-dimensional pooling combined with amplicon stacking and nextgeneration sequencing to identify mutations in polyploid oilseed rape in a fast and cost-effective manner for complex metabolic pathways. Furthermore, the mutants identified in Bn2-PGK2 might be a very valuable resource for industrial production of oilseed rape protein for human consumption.

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Polypeptide composition of major oilseed proteins and functional properties of extracted protein products: A concise review

Aluko

2023

J Americ Oil Chem Soc

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Oilseeds are grown mainly for their oil content but the residues (meals) that remain after defatting are excellent sources of plant protein ingredients. However, to serve as useful ingredients, the extracted proteins must meet industry expectations in terms of functional performance. Protein functionality is influenced by structural conformation, amino acid composition, type of polypeptides, presence of non‐protein materials (carbohydrates, lipids, and polyphenols), which in turn can be modified by the extraction method. Defatted oilseed meals are extracted mostly through the pH shift method, which involves alkaline solubilization followed by acid‐induced protein precipitation at the isoelectric point. A less popular method is called the protein micellar mass whereby the oilseed meal proteins are extracted with a NaCl solution, which is later diluted to reduce the ionic strength to a level where the proteins are no longer soluble and hence precipitate. A third method utilizes carbohydrases and phytases to first digest non‐protein materials from the oilseed meal into smaller units that are then removed by membrane ultrafiltration to leave behind a protein‐rich extract. These methods produce mainly two types of isolated oilseed proteins, concentrates (60%–89% protein content) and isolates (≥90% protein content), which can differ in terms of their protein conformation, solubility, and functionality as food ingredients. Therefore, this review provides an overview of the extraction and isolation as well as structural and functional properties of soybean, peanut, canola, hemp seed, sunflower, and sesame seed proteins.

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Increase of protein extraction yield from rapeseed meal through a pretreatment with phytase

Cited by 36 publications

References 20 publications

Establishment of a rapeseed meal fermentation model for iturin A production by Bacillus amyloliquefaciens CX‐20

Establishment of a rapeseed meal fermentation model for iturin A production by Bacillus amyloliquefaciens CX‐20

Identification of phytic acid mutants in oilseed rape (Brassica napus) by large‐scale screening of mutant populations through amplicon sequencing

Polypeptide composition of major oilseed proteins and functional properties of extracted protein products: A concise review

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