Increase of ruminal fiber digestion by cellobiose and a twin strain of Saccharomyces cerevisiae live cells in vitro

Abstract

This experiment was designed to investigate the effects of different concentrations of cellobiose (CB) or a twin strain of Saccharomyces cerevisiae live cells (YST) (20, 40 and 60 mg/60 mL), and CB + YST (60 + 20, 60 + 40, 60 + 60 mg/60 mL) on mixed ruminal microorganism fermentation in vitro. Ruminal fluid was collected from a cow, mixed with phosphate buffer (1:2) and incubated (60 mL) anaerobically at 38°C for 24 h with or without supplement plus 400 mg (dry matter [DM] basis) substrate (hay plus concentrate, 1.5:1). The medium pH numerically decreased with CB and CB + YST, but was unchanged with YST. The total volatile fatty acid and proportion of propionate increased (P < 0.05) in all cases. The proportion of acetate decreased (P < 0.05) with CB and CB + YST, but increased (P < 0.05) with YST and that of butyrate increased (P < 0.05) with CB and CB + YST, but decreased (P < 0.05) with YST. Ammonia-N decreased (P < 0.05) with CB and CB + YST, but was unchanged with YST. The number of protozoa was unchanged, and that of cellulolytic bacteria increased (P < 0.05) in all cases. Total gas production increased (P < 0.05) in all cases. Methane decreased, hydrogen was unchanged by YST and both gases were unchanged by CB and CB + YST. The in vitro disappearance of DM and neutral detergent fiber increased (P < 0.05) by 11.2% and 8.9%, 9% and 8.5%, and 12.1% and 10.2% in the case of CB, YST and CB + YST, respectively. Therefore, the dietary supplementation of CB and/or YST may improve ruminal fermentation and digestibility.

Degradabilidade de gramíneas, fermentação e protozoários no rúmen de bovinos em dietas com diferentes aditivos

“…Houve diferenças significativas entre os tratamentos e entre os capins avaliados na degradabilidade ruminal dos nutrientes, entretanto, foi observado interação significativa entre tratamentos e os capins, para: (1) a fração potencialmente degradável (b) da MS do capim Brachiaria nos animais suplementados com a monensina (57,91%) e com levedura (59,19%) foi menor (P<0,05) em relação à dieta sem uso de aditivos (63,94%) enquanto que o tratamento Fator P (62,12%) não diferiu entre os demais (Tabela 1); (2) houve diminuição da fração (b) da MS da silagem de Mombaça com a monensina (53,47%) em relação a dieta controle (57,20%), levedura (56,71%) e Fator P (57,96%) (Tabela 1); (3) a taxa de degradação da proteína bruta do Brachiaria foi menor nos tratamentos Controle e Fator P (2,43%/h) do que em monensina (4,93%/h) e levedura (4,37%/h) (Tabela 2); (4) a taxa de degradação da PB da silagem de Mombaça foi maior com a monensina (6,56%/h) em relação ao controle (2,59%/h), o qual não diferiu com a levedura (3,87%/h) e com o Fator P (3,88%) (Tabela 2); (5) A monensina promoveu maior taxa de degradação (c) da PB com o Brachiaria e com a silagem de Mombaça (p<0,05) em relação ao tratamento sem aditivos mas sem efeito para o Tifton-85; (6) (LILA et al, 2006;CHAUCHEYRAS-DURAND et al, 2016). No presente experimento, esse efeito não foi observado uma vez que não houve diferenças nas degradabilidades potencial e efetiva da FDN entre os tratamentos.…”

Effect of cellooligosaccharide or synbiotic feeding on growth performance, fecal condition and hormone concentrations in Holstein calves

“…Kobayashi and Itabashi (1987) have reported an increase in plasma insulin concentrations by intraruminal and intraduodenal infusion of propionate and butyrate in weaned calves. Lila et al (2006) reported that CE increased the number of cellulolytic bacteria and fiber digestibility with the increase in propionate and total VFA production. This may be due to increased absorption of propionate and butyrate from the digestive tract.…”

Yeasts in modern biotechnology

“…д.). Для того, чтобы синтезировать протеин своего тела животное должно получать необходимое количе-ство протеина в составе пищи [15].…”