Increase of stratifin triggered by ultraviolet irradiation is possibly related to premature aging of human skin

Murakami, Yuhko; Tanaka, Hiroshi; Nakata, Satoshi

doi:10.1111/exd.12390

Cited by 19 publications

(20 citation statements)

References 23 publications

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“…Although we did not detect specific mTOR‐associated proteins, increased mTOR activity results in reduced autophagy, a process known to be essential for keratinocyte differentiation . However, certain proteins are increased on the cheek indicative of enhanced mTOR activity, for example, increased levels of the adapter signalling protein, stratifin (14‐3‐3 σ ) were observed on the cheek . Raaby et al .…”

Section: Discussioncontrasting

confidence: 52%

“…Although we did not detect specific mTOR-associated proteins, increased mTOR activity results in reduced autophagy, a process known to be essential for keratinocyte differentiation [59,60]. However, certain proteins are increased on the cheek indicative of enhanced mTOR activity, for example, increased levels of the adapter signalling protein, stratifin (14-3-3r) were observed on the cheek [61]. Raaby et al [62] reported increased stratifin expression in psoriasis and contact dermatitis, and its levels are also reported to be increased following UV irradiation leading to decreased profilaggrin expression [61].…”

Section: Discussionmentioning

confidence: 62%

“…Raaby et al . reported increased stratifin expression in psoriasis and contact dermatitis, and its levels are also reported to be increased following UV irradiation leading to decreased profilaggrin expression . Thus, increased mTOR activity is likely in the cheek samples.…”

Section: Discussionmentioning

confidence: 93%

“…However, certain proteins are increased on the cheek indicative of enhanced mTOR activity, for example, increased levels of the adapter signalling protein, stratifin (14-3-3r) were observed on the cheek [61]. Raaby et al [62] reported increased stratifin expression in psoriasis and contact dermatitis, and its levels are also reported to be increased following UV irradiation leading to decreased profilaggrin expression [61]. Thus, increased mTOR activity is likely in the cheek samples.…”

Section: Discussionmentioning

confidence: 99%

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The effect of photodamage on the female Caucasian facial stratum corneum corneome using mass spectrometry‐based proteomics

Voegeli

Monneuse²,

Schoop

et al. 2017

Intern J of Cosmetic Sci

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BACKGROUND:The effect of photodamage on facial stratum corneum (SC) is still poorly understood. OBJECTIVE: To describe the SC proteome from tape strippings of Caucasian SC from photoexposed cheek and photoprotected postauricular (PA) site, a global analysis of photodamage on the skin will be developed leading to a better understanding of keratinocyte signalling pathways and identification of new molecular targets for the treatment of photoaged skin. METHODS: Female Caucasian subjects had nine consecutive tape strippings taken from their cheeks and PA site. Proteins were extracted and the trypsin-digested peptides were analysed by nanochromatography coupled to a high-resolution mass spectrometer. Data-dependent acquisition allowed protein identification that was processed by Paragon algorithm of Protein Pilot software. RESULTS: Changes in the levels of epidermal differentiation proteins were apparent indicating poor epidermal differentiation and SC maturation (keratins, cornified envelope (CE) proteins) on photoexposed cheeks. Differences in protease-anti-protease balance were observed for corneodesmolysis (favouring desquamation) and filaggrinolysis (favouring reduced filaggrin processing). 12R-LOX, a CE maturation enzyme, was reduced in photodamaged skin but not transglutaminases. Changes in signal keratinocyte transduction pathway markers were demonstrated especially by reduced levels of downstream signalling markers such as calreticulin (unfolded protein response; UPR) and increased level of stratifin (target of rapamycin; mTOR). Evidence for impaired proteostasis was apparent by reduced levels of a key proteasomal subunit (subunit beta type-6). Finally, key antioxidant proteins were upregulated except catalase. CONCLUSION: Clear examples of poor keratinocyte differentiation and associated metabolic and signalling pathways together with reduced SC maturation were identified in photodamaged facial SC. Corneocyte immaturity was evident with changes in CE proteins. Particularly, the reduction in 12R-LOX is a novel finding in photodamaged skin and supports the lack of SC maturation. Moreover, filaggrinolysis was reduced, whereas corneodesmolysis was enhanced. From our results, we propose that there is a poor crosstalk between the keratinocyte endoplasmic reticulum UPR, proteasome network and autophagy machinery that possibly leads to impaired keratinocyte proteostasis. Superimposed on these aberrations is an apparently enhanced mTOR pathway that also contributes to reduced SC formation and maturation. Our results clearly indicate a corneocyte scaffold disorder in photodamaged cheek SC.R esum e CONTEXTE: Les effets des dommages dus a l'exposition a la lumiere sur le stratum corneum (SC) sont encore mal compris. OBJECTIFS: D ecrire le prot eome du SC issu de pr el evements par bandes adh esives provenant de la joue, expos ee a la lumi ere, et de la r egion post-auriculaire, prot eg ee de la lumi ere, de sujets caucasiens.A cette fin, une analyse globale des dommages cutan es dus a l'exposition a la lumi er...

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Section: Discussioncontrasting

confidence: 52%

Section: Discussionmentioning

confidence: 62%

Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

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The effect of photodamage on the female Caucasian facial stratum corneum corneome using mass spectrometry‐based proteomics

Voegeli

Monneuse²,

Schoop

et al. 2017

Intern J of Cosmetic Sci

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“…UVB‐induced photoageing is known to be mediated by increased MMP expression and activation along with collagen degradation . To determine the effects of UVB on MMP expression, we measured changes in the levels of photoageing‐related MMPs.…”

Section: Resultsmentioning

confidence: 99%

Inhibition of hedgehog signalling attenuates UVB‐induced skin photoageing

Kim

Yang

et al. 2015

Experimental Dermatology

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The hedgehog (Hh) signalling pathway regulates normal development and cell proliferation in metazoan organisms, but its aberrant activation can promote tumorigenesis and progression of a variety of aggressive human cancers including skin cancer. Despite its importance, little is known about its role in photoageing, a type of UV-induced skin lesions. In this study, we investigated the involvement of Hh signalling in the photoageing process as well as the use of an Hh-regulating alkaloid compound as a novel therapeutic drug to regulate photoageing in keratinocytes. We found that UVB induced Hh signalling by the expression of Hh ligands and Hh-mediated transcription factors, respectively. Moreover, UVB-induced Hh activation relied on mitogen-activated protein kinase (p38, ERK and JNK) activity and inflammatory responses (upregulation of COX-2, IL-1β, IL-6 and TNF-α), resulting in premature senescence and photoageing in vitro and in vivo. Notably, a selected Hh inhibitor, evodiamine, mediated photoageing blockade in a mouse skin model. Taken together, our findings demonstrated that Hh signalling is associated with UVB-induced photoageing, while pharmacological inhibition of Hh signalling significantly reduced experimental photoageing, indicating its potential for use as a therapeutic target for this disease.

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High Rejection‐Ratio IGZO Ultraviolet Phototransistor via Additional Solution Processed ZnO Absorption Layer

Ma,

Jeong,

Kang

et al. 2024

Advanced Optical Materials

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The depletion of the ozone layer over the last four decades has increased Earth's exposure to ultraviolet (UV) radiation, posing significant health risks. Indium‐Gallium‐Zinc‐Oxide (IGZO) phototransistors have emerged as promising UV detection candidates, boasting a wide bandgap (>3 eV), low off‐current, high on‐current, and stability. However, previous efforts to enhance the photoresponse of IGZO phototransistors focused on visible light absorption layers, neglecting UV detection. Here, zinc oxide (ZnO) nanoparticles (NPs) as an absorption layer in IGZO TFTs for UV phototransistors are introduced. IGZO/ZnO TFTs exhibit robust photoresponsivity for UV light below 360 nm, showcasing rejection ratios (Ruv / Rvis) of 20 258, 62 261, 193 649, and 401 582 for wavelengths of 360, 340, 320, and 300 nm, respectively. The combination of IGZO's wide bandgap with ZnO NPs proves effective for detecting UV light below 360 nm, even at low incident light intensities (15 µW cm−2). Enhanced photoresponse characteristics are confirmed via ultraviolet photoelectron spectroscopy. This research highlights the potential of IGZO materials for UV phototransistors and introduces a novel perspective on the utilization of an additional absorption layer traditionally employed for the conventional extension into the visible light range.

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Increase of stratifin triggered by ultraviolet irradiation is possibly related to premature aging of human skin

Cited by 19 publications

References 23 publications

The effect of photodamage on the female Caucasian facial stratum corneum corneome using mass spectrometry‐based proteomics

The effect of photodamage on the female Caucasian facial stratum corneum corneome using mass spectrometry‐based proteomics

Inhibition of hedgehog signalling attenuates UVB‐induced skin photoageing

High Rejection‐Ratio IGZO Ultraviolet Phototransistor via Additional Solution Processed ZnO Absorption Layer

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