Increase of the LC–MS/MS sensitivity and detection limits using on-line sample preparation with large volume plasma injection

Li, Ming; Alnouti, Yazen; Leverence, Rachael; Bi, Honggang; Gusev, Arkady I.

doi:10.1016/j.jchromb.2005.03.047

Cited by 29 publications

(12 citation statements)

References 21 publications

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“…On-line SPE technique method, compared to other offline SPE methods, has becoming particularly attractive these years because of numerous advantages, such as high throughput, no loss of analytes and small consumption of organic solvent, etc. To date, although on-line SPE coupled to LC/MS or LC/MS/MS has been widely applied to the trace analysis of organic compounds in environmental waters and biofluids [19][20][21], little information could be obtained about the determination method of steroid hormones in milk [22].…”

Section: Introductionmentioning

confidence: 99%

Determination of estrogens and bisphenol A in bovine milk by automated on-line C30 solid-phase extraction coupled with high-performance liquid chromatography–mass spectrometry

Yan

Zhao

et al. 2009

Journal of Chromatography A

108

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a b s t r a c tUsing triacontyl bonded silica (C 30 ) as on-line solid-phase extraction (SPE) material and a specially designed on-line analytical system which allowed large sample volume injection, a high speed and robust on-line SPE-HPLC-MS method was established for the analysis of five estrogens and bisphenol A (BPA) in milk samples. The milk sample is pretreated with acetonitrile for protein precipitation and then treated with primary secondary amine for the removal of polar impurities in the matrix. Then the pretreated sample can be automatically loaded by a LC pump. For effective extraction, an offshoot with NH 4 Ac solution of high-flow rate was employed to dilute the loaded sample by a mixing tee before sample was loaded onto the C 30 extraction column. In this way, large volume injection (1 mL in this experiment) could be achieved. Some important parameters such as sample loading flow rate, sample dilution ratio and injection volume were optimized. Under the optimized conditions, the recoveries for all analytes range from 71.4 to 97.1% and reproducibility represented as RSDs are less than 15.0% (n = 5) with milk samples spiked at 0.6 and 15 ng/mL of each analyte. To the authors' knowledge, it constitutes the first work describing a C 30 on-line SPE-LC-MS analytical method for the screen and monitoring of these estrogens and BPA in milk.

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Section: Introductionmentioning

confidence: 99%

Determination of estrogens and bisphenol A in bovine milk by automated on-line C30 solid-phase extraction coupled with high-performance liquid chromatography–mass spectrometry

Yan

Zhao

et al. 2009

Journal of Chromatography A

108

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“…To accommodate the dilution effect of the in-solution trypsinization, we investigated a combination of moderately miniaturized chromatography and large-volume injection in a column switching/trapping setup. Large-volume injection procedures have already proven to be very adequate to raise the sensitivity limits of a given system in GC [8,9] and gain more and more interest in LC too. The use of a column-switching valve is not only profitable to inject a larger amount of sample but also, as an extra advantage, desalting and/or a certain level of sample clean-up is achieved at the same time.…”

Section: Lc (Tandem) Ms (Lc-ms(/msmentioning

confidence: 99%

Improved analyte detectability of proteins and peptide lysates by means of multiple large‐volume injection in LC‐MS

Storme

t’Kindt

Bocxlaer³

2009

J of Separation Science

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A 'multiple (trapping) large-volume injection' approach was developed for the analysis of peptides and proteins. In this way, a maximally 10-fold gain in sensitivity could be achieved. The system involves the use of an automated 10-port switching valve in combination with a 1 mm i.d. trapping/guard column and a 1 mm i.d. x 150 mm analytical column. The optimized multiple injection/loading procedure allows quantitative measurements of peptides and protein lysates. Linear calibration curves (R(2) > or = 0.988) over a minimum of two orders of magnitude were generated for a range of peptide and protein standards with sensitivities equal to or even exceeding, those generally achieved only through increasing miniaturization (quantification limit > or = 0.5 pmol/mL).

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“…Many research groups have improved in-use assay technologies. [1][2][3] Some other groups have explored advanced technologies. [4][5][6][7] Several years ago, some authors proposed an assay technology called immunomagnetic reduction (IMR).…”

Section: Introductionmentioning

confidence: 99%

Feasibility studies for assaying alpha-fetoprotein using antibody-activated magnetic nanoparticles

Huang¹,

Yang

Hong³

et al. 2012

IJN

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Some previous reports have already shown the characterizations of immunomagnetic reduction (IMR). The assay technology involves the utilities of biofunctionalized magnetic nanoparticles to label target biomolecules. However, the detection threshold and interference tests for IMR have not been investigated in detail. In this study, alpha-fetoprotein (AFP) was used as a target biomolecule. The signals for AFP solutions of various concentrations, or with interfering materials, were detected via IMR. These samples were also used for characterizing the detection threshold and interference with enzyme-linked immunosorbent assay (ELISA). The results of assaying AFP level with IMR and ELISA were compared. The detection threshold for assaying AFP with IMR was found to be 3 ng/mL, which is 15 times lower than that of ELISA, and definitely suppresses false negative. For the interfering materials noted commonly in serum such as hemoglobin, bilirubin, triglyceride, and vascular endothelial growth factor, there was no detectable interfering effect when assaying AFP with IMR. Several serum samples from normal people and liver-tumor-bearing patients were used for the detections of AFP concentration via IMR. These results reveal the feasibilities of assaying AFP in blood using IMR, as well as achieving high-sensitive and high-specific assay for AFP.

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Increase of the LC–MS/MS sensitivity and detection limits using on-line sample preparation with large volume plasma injection

Cited by 29 publications

References 21 publications

Determination of estrogens and bisphenol A in bovine milk by automated on-line C30 solid-phase extraction coupled with high-performance liquid chromatography–mass spectrometry

Determination of estrogens and bisphenol A in bovine milk by automated on-line C30 solid-phase extraction coupled with high-performance liquid chromatography–mass spectrometry

Improved analyte detectability of proteins and peptide lysates by means of multiple large‐volume injection in LC‐MS

Feasibility studies for assaying alpha-fetoprotein using antibody-activated magnetic nanoparticles

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