Increase of the yields of eicosapentaenoic and docosahexaenoic acids by the microalga <i>Pavlova lutheri</i> following random mutagenesis

Meireles, Luís A.; Guedes, A. Catarina; Malcata, F. Xavier

doi:10.1002/bit.10451

Cited by 67 publications

(36 citation statements)

References 22 publications

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“…The influence of light intensity on the lipid profile of Pavlova lutheri showed that cultures grown under low light intensity (9 Wm -2 ) had a higher fraction of EPA and DHA esterified in polar classes which had a favorable role in aquaculture (Catarina Guedes et al 2010). Application of the genetic engineering concept, by using u.v.-light as mutagenic agent generated strains with EPA and DHA contents about 32.8 and 32.9% (in % dry biomass) higher than those of the control, native strain (Meireles et al 2003). Use of process engineering, an on-line based controlled process in a model system has been employed to increase the production rates of both EPA and DHA by P. lutheri under various conditions of light and growth rate (Meireles et al 2008).…”

Section: Introductionmentioning

confidence: 94%

Microalgae: a sustainable feed source for aquaculture

Hemaiswarya

Raja

Kumar

et al. 2010

World J Microbiol Biotechnol

351

176

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The need for nutritional sources safer than traditional animal products has renewed interest generally in plants and particularly in microalgae. Microalgae have diverse uses in aquaculture, their applications are mainly to provide nutrition and to enhance the colour of the flesh of salmonids. The larvae of molluscs, echinoderms and crustaceans as well as some fish larvae feed on microalgae. Several studies have confirmed that a live multi-specific, low bacterial and microalgal biomass remains essential for shellfish hatcheries. Major advances are expected from new production system, designs and operations from batch run open tanks to more sophisticated continuously-run and closed loop reactors. Currently, studies are underway to examine the cost-effectiveness of the on-and off-site microalgal production systems which can only be achieved by substantial scaling-up and improved quality control. In order to attain sustainability in the usage of microalgae, a systems-based approach is required which integrates different fields such as biotechnology, bioprocess and management procedures.

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Section: Introductionmentioning

confidence: 94%

Microalgae: a sustainable feed source for aquaculture

Hemaiswarya

Raja

Kumar

et al. 2010

World J Microbiol Biotechnol

351

176

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“…Often high doses of mutagen are applied to kill most microorganisms in order to increase frequency of generated mutants (Demain and Adrio 2008;Meireles et al 2002;Smith and Wood 1991). However, the optimum dose of mutagen is that which gives the highest proportion of desirable mutants in the surviving population.…”

Section: Introductionmentioning

confidence: 99%

Microbial strain improvement for enhanced polygalacturonase production by Aspergillus sojae

Heerd

Tarı

Fernández‐Lahore

2014

Appl Microbiol Biotechnol

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Strain improvement is a powerful tool in commercial development of microbial fermentation processes. Strains of Aspergillus sojae which were previously identified as polygalacturonase producers were subjected to the costeffective mutagenesis and selection method, the so-called random screening. Physical (ultraviolet irradiation at 254 nm) and chemical mutagens (N-methyl-N′-nitro-Nnitrosoguanidine) were used in the development and implementation of a classical mutation and selection strategy for the improved production of pectic acid-degrading enzymes. Three mutation cycles of both mutagenic treatments and also the combination of them were performed to generate mutants descending from A. sojae ATCC 20235 and mutants of A. sojae CBS 100928. Pectinolytic enzyme production of the mutants was compared to their wild types in submerged and solid-state fermentation. Comparing both strains, higher pectinase activity was obtained by A. sojae ATCC 20235 and mutants thereof. The highest polygalacturonase activity (1,087.2±151.9 U/g) in solid-state culture was obtained by mutant M3, which was 1.7 times increased in comparison to the wild strain, A. sojae ATCC 20235. Additional, further mutation of mutant M3 for two more cycles of treatment by UV irradiation generated mutant DH56 with the highest polygalacturonase activity (98.8±8.7 U/mL) in submerged culture. This corresponded to 2.4-fold enhanced polygalacturonase production in comparison to the wild strain. The results of this study indicated the development of a classical mutation and selection strategy as a promising tool to improve pectinolytic enzyme production by both fungal strains.

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“…However, some common characteristics of the strains, such as degeneration, weak adaptability, and low production, still hinder the large-scale production [6]. As a kind of simple and effective breeding strategy, mutagenesis is widely used for selecting various kinds of high-producing strains, Meireles [4] increased the yields of EPA and DHA by the microalga following mutagenesis, and it had also been shown to improve the EPA content of microalgae Phaeodactylum tricornutum via ultraviolet (UV) mutant [7].…”

mentioning

confidence: 99%

“…isolated from the ocean has attracted increasing attention. Although the industrial production of DHA has been achieved at very large scale, some methods were for attempts to improve DHA synthesis via intrinsic parameters [4] or extrinsic parameters [5]. However, some common characteristics of the strains, such as degeneration, weak adaptability, and low production, still hinder the large-scale production [6].…”

mentioning

confidence: 99%

Increase of Docosahexaenoic Acid Production by Schizochytrium sp. Through Mutagenesis and Enzyme Assay

Liu

Huang

Ren

et al. 2009

Appl Biochem Biotechnol

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The present study focused on improving docosahexaenoic acid (DHA) production by Schizochytrium sp. through N-methyl-N-nitro-N-nitrisiguanidine treatment coupled with ultraviolet radiation based on the metabolic pathway analysis. The activity of glucose-6-phosphate dehydrogenase of the mutant was higher than the parent strain, which indicated that the hexose monophosphate pathway of the mutant was strengthened, and more NADPH was thus produced. Also, the activities of malic enzyme and ATP-citrate lyase in the cell extract of the mutant were higher than the parent strain, which indicated that the screening method increased NADPH and acetyl-CoA supply in vivo effectively. Finally, in the batch culturing of the mutant, 34.84% higher lipid was accumulated with the cell dry weight at the same level compared with the parent strain. Moreover, the DHA percentage of the total fatty acids up to 56.22% was achieved using the mutant, which was 38.88% higher than the parent strain. When the cultures were maintained under appropriate conditions, the final DHA yield was 0.20 and 0.11 g/g dry biomass, for the mutant and parent, respectively.

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Increase of the yields of eicosapentaenoic and docosahexaenoic acids by the microalga Pavlova lutheri following random mutagenesis

Cited by 67 publications

References 22 publications

Microalgae: a sustainable feed source for aquaculture

Microalgae: a sustainable feed source for aquaculture

Microbial strain improvement for enhanced polygalacturonase production by Aspergillus sojae

Increase of Docosahexaenoic Acid Production by Schizochytrium sp. Through Mutagenesis and Enzyme Assay

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