Increased activation-induced cell death of high IFN-γ–producing TH1 cells as a mechanism of TH2 predominance in atopic diseases

Akkoç, Tunç; Koning, Pieter J.A. de; Rückert, Beate; Barlan, Işıl; Akdiş, Mübeccel

doi:10.1016/j.jaci.2007.12.1171

Cited by 96 publications

(83 citation statements)

References 39 publications

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“…Akkoc et al suggests that Th1 lymphocytes are more sensitive to Fas mediated programmed cell death than Th2 [13]. The same suggestion appears in Zhang's paper [14].…”

Section: Discussionmentioning

confidence: 78%

See 1 more Smart Citation

Lymphocytes sensitivity to Fas stimulation in healthy and asthmatic children.

Potapińska

Zawadzka-Krajewska²,

Kotuła³

et al. 2010

Folia Histochem Cytobiol.

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Abstract:The T cell hypothesis of asthma is based on the concept that the disease is driven and maintained by the persistence of a specialized subset of chronically activated T memory cells sensitized against an array of allergenic, occupational or viral antigens. Overreaction of CD4+ T cells in the peripheral blood and airway tissues is an invariant feature of asthma; therefore a potent mechanism for augmenting the number of activated T cells in this disease would be the resistance to the normally programmed pathway for cell death. The aim of the study was to evaluate the presence of apoptotic markers on peripheral blood lymphocytes from healthy and asthmatic children before and after stimulation with antiCD95 antibodies. The blood was collected from 21 children with atopic asthma suffering from allergic rhinitis because of house dust mite and/or grass pollen allergens and 8 healthy children matched for their age and sex. Blood was mixed with purified monoclonal antibody antiCD95 (Beckman Coulter), incubated for 24 hours and than stained with Annexin V andPI (Becton Dickinson). Prepared suspensions were analyzed with Cytomics FC 500 (Beckman Coulter) flow cytometer. Annexin V(+)/PI(-) cells were characterized as early apoptotic, Annexin V(+)/PI(+) as late apoptotic and Annexin V(-)/PI(+) as dead. In unstimulated sample from asthmatic children 21.09±11.20% cells were characterized as Annexin V positive/PI negative. After stimulation with antiCD95 Annexin V positive/PI negative cells constituted 18.72±9.42% of cells, p=0.1. In unstimulated sample from healthy children 11.69±6.70% cells were characterized as Annexin V positive/PI negative. In the sample stimulated with antiCD95 16.54±2.98% of cells were Annexin V positive/PI negative, p=0.02. There were no differences between results of late apoptotic and necrotic lymphocytes from healthy and asthmatic children. Performed research indicates that lymphocytes from asthmatic children are resistant to Fas mediated apoptosis.

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“…Akkoc et al suggests that Th1 lymphocytes are more sensitive to Fas mediated programmed cell death than Th2 [13]. The same suggestion appears in Zhang's paper [14].…”

Section: Discussionmentioning

confidence: 78%

“…It is proposed that IFN-γ produced by Th1 cells influences caspases expression and sensitizes cells to the apoptosis. To confirm that hypothesis Akkoc et al proved that absence of IFN-γ protects Th1 lymphocytes against Fas -mediated apoptosis [13].…”

Section: Discussionmentioning

confidence: 91%

Lymphocytes sensitivity to Fas stimulation in healthy and asthmatic children.

Potapińska

Zawadzka-Krajewska²,

Kotuła³

et al. 2010

Folia Histochem Cytobiol.

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show abstract

“…The main effector cells are the eosinophils and mast cells. Predominance of Th2 cells might be caused by an increased tendency to activate induced cell death of high IFN-gama producing Th1 cells as it is commonly observed in patients with atopic disorders (15). Th1 cells induce apoptosis of keratinocytes in atopic dermatitis and epithelial cells and/or smooth muscle cells in asthma in the effector phase of these allergic diseases (16)(17)(18)(19)(20).…”

Section: A Ll E Rgymentioning

confidence: 99%

Histamine and Antihistamines / Histamin i antihistamini

Stojkovic¹,

Cekić²,

Ristov³

et al. 2015

Acta Facultatis Medicae Naissensis

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“…Aside from Th2 type cells different T-cells subsets evolve from naïve T-cell subsets into other T-cell subsets, depending on the microenvironment. Th1-cells secrete IFN-γ which induces apoptosis in concert with TNFα in epithelial cells, smooth muscle cells in the lungs of asthmatic patients and in keratinocytes of patients with atopic dermatitis [2][3][4] and in activated Th1, but not Th2-cells. This may contribute to the limitation of inflammatory responses via elimination of cytokine and chemokine producing cells.…”

Section: Introductionmentioning

confidence: 99%