Increased activity of lymph node cells in experimental thermal injury: changes in accessory cells in injured area-draining lymph nodes

Kataranovski, Milena; Nikolić, Tatjana; Veličković, Maja; Čolić, Miodrag; Pejnović, Nada; Vojinović, Jelena

doi:10.1016/s0305-4179(00)00018-8

Cited by 2 publications

(1 citation statement)

References 29 publications

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“…Many studies have focused on monocyte/macrophage functions after burn injury but little is known about DC's involvement in immune regulation in the post-injury period [38]. In an experimental burn model, Kataranovski et al [41] have noted an increase of DCs in wound-draining lymph nodes at 24-72 h after injury. More recently, Lederer's group showed that burn injury-primed macrophages and dendritic cells for enhanced TLR2 and TLR4-induced IL-1b, IL-6, and TNF-a production in the spleen [34].…”

Section: Discussionmentioning

confidence: 99%

Selective effect of burn injury on splenic CD11c+ dendritic cells and CD8α+CD4−CD11c+ dendritic cell subsets

Patenaude

D’Elia

Hamelin

et al. 2010

Cell. Mol. Life Sci.

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Burn injury causes an immunosuppression associated with suppressed adaptive immune function. Dendritic cells (DCs) are APCs for which signaling via their Toll-like receptors (TLRs) induces their maturation and activation, which is essential for the adaptive immune response. In this study, we examined if burn injury alters the TLR activity of splenic DCs. After injury, we noticed that DC functions were impaired, characterized by a suppressed capacity to prime naive T cells when triggering the TLR4 signaling cascade using specific ligands (LPS or rHSP60). The observed perturbations on LPS-primed DCs isolated from burned mice exhibited significantly diminished IL-12p40 production and enhanced IL-10 secretion-associated impairment in mitogen-activated protein kinase activation. Interestingly, we observed a decrease of TLR4/MD-2 expression on the CD8alpha(+) DC subset that persisted following LPS stimulation. The altered TLR4 expression on LPS-stimulated CD8alpha(+) DCs was associated with reduced capacity to produce IL-12 after stimulation. Our results suggested that TLR4 reactivity on DCs, especially CD8alpha(+) DCs, is disturbed after burn injury.

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Section: Discussionmentioning

confidence: 99%