Increased apoptosis in cryopreserved autologous hematopoietic progenitor cells collected by apheresis and delayed neutrophil recovery after transplantation: a nested case-control study

Wu, Luke; Alhejazi, Ayman; Filion, Lionel G.; Ben, Robert N.; Halpenny, Mike; Yang, Lin; Giulivi, Antonio; Allan, David S.

doi:10.3109/14653249.2011.610302

Cited by 15 publications

(16 citation statements)

References 24 publications

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“…Unlike umbilical cord blood and autologous grafts that are processed and volume-reduced before cryopreservation and storage, grafts from adult donors are typically collected as close to the date of infusion as possible and administered fresh to avoid losses in cell yield and/or loss of cell function that could affect immune reconstitution and transplant outcomes such as engraftment. 3,4 Moreover, infusing cryoprotectants such as dimethyl sulfoxide can be associated with infusion-related toxicity and/or may require washing of the product, which may further impact cell dose of the product. Importantly, the infusion of fresh cells may reduce costs associated with processing, cryopreservation, and storage.…”

mentioning

confidence: 99%

Cryopreservation of adult unrelated donor products in hematopoietic cell transplantation: the OneMatch experience and systematic review of the literature

et al. 2017

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mentioning

confidence: 99%

Cryopreservation of adult unrelated donor products in hematopoietic cell transplantation: the OneMatch experience and systematic review of the literature

et al. 2017

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“…11,[14][15][16][17][18] The Ben laboratory has been designing and synthesizing small molecule IRIs and has demonstrated their advantages in many in vitro cellular systems. 4,[19][20][21][22][23] The structure of aldonamide-derived IRI is shown in Fig. 1.…”

Section: Resultsmentioning

confidence: 99%

Inhibition of ice recrystallization during cryopreservation of cord blood grafts improves platelet engraftment

et al. 2020

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BACKGROUND Platelet engraftment following cord blood (CB) transplantation remains a significant hurdle to this day. The uncontrolled growth of ice, a process referred to as ice recrystallization, is one of several mechanisms that lead to cell loss and decreased potency during freezing and thawing. We hypothesized that reducing cell damage induced by ice recrystallization in CB units (CBUs) would reduce losses of stem and progenitor cells and therefore improve engraftment. We previously demonstrated that the ice recrystallization inhibitor (IRI) N‐(2‐fluorophenyl)‐D‐gluconamide (IRI 2) increases the postthaw recovery of CB progenitors. Herein, we set out to ascertain whether IRI 2 can enhance platelet and bone marrow engraftment activity of hematopoietic stem cells (HSCs) in cryopreserved CBUs using a serial transplantation model. STUDY DESIGN AND METHODS CBUs were processed following standard volume/red blood cell reduction procedure and portions frozen with dimethyl sulfoxide (DMSO) supplemented or not with IRI 2. Thawed CB samples were serially transplanted into immunodeficient mice. RESULTS Our results show that supplementation of DMSO with IRI 2 had several beneficial effects. Specifically, higher levels of human platelets were observed in the peripheral blood (p < 0.05; n = 4) upon transplant of CBUs preserved with the IRIs. In addition, human BM chimerism and the number of human CFU progenitors in the bone marrow were superior in IRI 2 recipients compared to DMSO recipients. Moreover, IRI 2 had no negative impact on the multilineage differentiation and self‐renewal activities of HSCs. DISCUSSION Taken together, these results demonstrate that supplementation of a hematopoietic graft with IRI can improve the postthaw engraftment activities of HSCs.

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“…The CD34 counts were performed as previously described. Colony-forming units were enumerated using an automated counter (Stem Cell Technologies, Vancouver, Canada) using standard culture methods that estimate the longer-term engraftment potential of cord blood units, as described previously [16]. The number of CD34-positive per 10 3 TNC and the number of CFUs per 10 4 TNC were determined to assess enrichment for hematopoietic stem and progenitor cells.…”

Section: Discussionmentioning

confidence: 99%

Obstetrical and neonatal factors associated with optimal public banking of umbilical cord blood in the context of delayed cord clamping

Munro

Corsi

Martin

et al. 2019

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Purpose: To assess the association of specific newborn and maternal factors with indicators of increased blood-forming capacity in umbilical cord blood to inform strategic collection strategies that could augment the quality of units in public cord blood banks. Methods: Data regarding 268 consecutive cord blood units (CBUs) banked by Canadian Blood Services were analyzed. Multivariate analysis was performed to identify factors associated with markers of hematopoietic potency and likelihood of utilization. Results: Delayed clamping of the cord beyond 60 s was associated with reduced volume collected. Any delay in clamping of the cord was associated with reduced total nucleated cell counts. Newborn weight >4,000 g was also associated with greater blood volume in the collection but not with other measures of hematopoietic potency. Cord blood acidosis at birth (pH

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Increased apoptosis in cryopreserved autologous hematopoietic progenitor cells collected by apheresis and delayed neutrophil recovery after transplantation: a nested case-control study

Cited by 15 publications

References 24 publications

Cryopreservation of adult unrelated donor products in hematopoietic cell transplantation: the OneMatch experience and systematic review of the literature

Cryopreservation of adult unrelated donor products in hematopoietic cell transplantation: the OneMatch experience and systematic review of the literature

Inhibition of ice recrystallization during cryopreservation of cord blood grafts improves platelet engraftment

Obstetrical and neonatal factors associated with optimal public banking of umbilical cord blood in the context of delayed cord clamping

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