Increased CCL24 and CXCL7 levels in the cerebrospinal fluid of patients with neurosyphilis

Li, Xin‐Xin; Zhang, Jing; Wang, Zhaoyuan; Chen, Si‐Qi; Zhou, Weifang; Wang, Tingting; Man, Xiao‐Yong; Zheng, Min

doi:10.1002/jcla.23366

Cited by 10 publications

(9 citation statements)

References 37 publications

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“…gastric cancer ( Chen et al, 2022 ) and thyroid carcinoma ( Zhang et al, 2021b )), inflammation ( e.g . rheumatoid arthritis ( Guerrero et al, 2021 )), neurosyphilis ( Li et al, 2020 ), COVID-19 ( Yatim et al, 2021 ), and metabolic disease ( e.g . diabetes mellitus ( Moin et al, 2021 ; Zhang et al, 2021a )).…”

Section: Discussionmentioning

confidence: 99%

PPBP gene as a biomarker for coronary heart disease risk in postmenopausal Thai women

Mansanguan

Maneerat

2022

PeerJ

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Background Estrogen is an important ovarian hormone with anti-atherogenic and cardioprotective effects. Postmenopausal women have lower estrogen levels, associated with significantly higher risks of coronary heart disease (CHD) and CHD-related death. Effective biomarkers for the diagnosis, prediction, and treatment of CHD are needed to address this problem and thus reduce the mortality due to CHD in postmenopausal women. We recently reported that the PPBP and DEFA1/DEFA3 genes may be feasible synergistic biomarkers for CHD risk in Thai men with hyperlipidemia. The PPBP gene encodes pro-platelet basic protein (PPBP) from activated platelets, and DEFA1/DEFA3 encodes human neutrophil peptides (HNP) 1–3, mainly produced by activated neutrophils. Both platelets and neutrophils are involved in chronic inflammation during the development of atherogenesis and CHD. This study investigated the potential roles of PPBP and DEFA1/DEFA3 and their proteins as biomarkers for CHD risk in postmenopausal Thai women. Methods This cross-sectional study enrolled 90 postmenopausal Thai women, including 12 healthy controls (N), 18 patients with hyperlipidemia (H), and 21 patients diagnosed with CHD. The remaining 39 women were receiving cholesterol-lowering drugs for hyperlipidemia (HD) were excluded from the study. All CHD patients underwent coronary bypass grafting or coronary angioplasty. PPBP and DEFA1/DEFA3 mRNA expression levels in peripheral blood mononuclear cells isolated from heparinized blood were determined by quantitative reverse-transcription polymerase chain reaction. Levels of PPBP and HNP-1–3 proteins in corresponding plasma samples were assessed by enzyme-linked immunosorbent assay. Differences in parameters were compared among groups and correlations between parameters and clinical manifestations were analyzed. Results PPBP mRNA and protein levels were significantly increased in the CHD group compared with the N and H groups. In contrast, DEFA1/DEFA3 mRNA and HNP-1–3 protein levels did not differ significantly among the groups. None of the levels were associated with any of the clinical parameters analyzed in this study. Conclusion The results indicate that gene and protein expression levels of PPBP, but not DEFA1/DEFA3, and HNP-1–3, may be feasible biomarkers for assessing CHD risk in postmenopausal Thai women with hyperlipidemia.

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Section: Discussionmentioning

confidence: 99%

PPBP gene as a biomarker for coronary heart disease risk in postmenopausal Thai women

Mansanguan

Maneerat

2022

PeerJ

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“…In addition, CXCL7 gene expression was upregulated in dendritic cells in response to B. garinii stimulation compared with unstimulated and LPS-stimulated cells [ 81 ], which might be due to platelet contamination however. During a different spirochetal infection, neurosyphilis, elevated CXCL7 concentrations in the cerebrospinal fluid were reported [ 82 ]. Finally, we found that the addition of recombinant CXCL7 increased cytokine production in an in vitro PBMC model of B. burgdorferi stimulation, supporting an active role for CXCL7 in the inflammatory response to B. burgdorferi.…”

Section: Discussionmentioning

confidence: 99%

Identifying platelet-derived factors as amplifiers ofB. burgdorferi-induced cytokine production

Kerstholt

Schoor

Oosting

et al. 2022

Clinical and Experimental Immunology

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Previous studies have shown that monocytes can be ‘trained’ or tolerized by certain stimuli to respond stronger or weaker to a secondary stimulation. Rewiring of glucose metabolism was found to be important in inducing this phenotype. As we previously found that Borrelia burgdorferi (B. burgdorferi), the causative agent of Lyme borreliosis (LB), alters glucose metabolism in monocytes, we hypothesized that this may also induce long-term changes in innate immune responses. We found that exposure to B. burgdorferi decreased cytokine production in response to the TLR4-ligand lipopolysaccharide (LPS). In addition, B. burgdorferi exposure decreased baseline levels of glycolysis, as assessed by lactate production. Using GWAS analysis, we identified a gene, microfibril-associated protein 3-like (MFAP3L) as a factor influencing lactate production after B. burgdorferi exposure. Validation experiments proved that MFAP3L affects lactate- and cytokine production following B. burgdorferi stimulation. This is mediated by functions of MFAP3L, which includes activating ERK2 and through activation of platelet degranulation. Moreover, we showed that platelets and platelet-derived factors play important roles in B. burgdorferi-induced cytokine production. Certain platelet-derived factors, such Chemokine C-X-C motif ligand 7 (CXCL7) and (C-C motif) ligand 5 (CCL5), were elevated in the circulation of LB patients in comparison to healthy individuals.

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“…CXCL7 has mostly been found to be expressed in tumor cells to promote their proliferation or migration [ 40 ]. CXCL7 released by vascular endothelial cells has been identified as a chemoattractant for human neural stem cells, with its level rising in the cerebrospinal fluid in bacterial meningitis and neurosyphilis [ 41 ]. To the best of our knowledge, this is the first study to show that CXCL7 has an extremely high expression in PVN of rats, although such expression decreased upon SIH.…”

Section: Discussionmentioning

confidence: 99%

Upregulation of Chemokines in the Paraventricular Nucleus of the Hypothalamus in Rats with Stress-Induced Hypertension

Chen

Zhang

et al. 2020

Med Sci Monit

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Background The neuroinflammation of paraventricular nucleus (PVN) of the hypothalamus has been implicated in the development of hypertension. The promoted invasion of peripheral immune cells into PVN may be attributed to the upregulation of chemokines, then exacerbating neuroinflammation. We studied the expressions of chemokines, activation of microglial cells, and inflammatory mediators in PVN of rats with stress-induced hypertension (SIH). Material/Methods SIH was induced by electrical foot shock combined with noise for 2 h twice a day, at an interval of 4 h for 14 consecutive days. At the end of the 14th day, fresh PVN tissues were collected to measure the expressions of chemokines using the RayBiotech antibody array. Results We are the first to report that the expression of CXCL7 was extremely high in PVN of control rats, and was significantly lower in SIH rats. The expressions of CCL2 and CX3CL1 in PVN of SIH rats significantly exceeded those of control rats. The numbers of CX3CR1 (receptor of CX3CL1)-immunostained cells and oxycocin-42 (OX-42, marker of microglia)-positive cells increased in PVN of the SIH rats. The stress enhanced the protein expressions of proinflammatory cytokines IL-6 and IL-17 and reduced those of anti-inflammatory cytokines TGF-β and IL-10 in PVN. Conclusions In PVN of SIH rats, chronic stress induced neuroinflammation characterized by the activated microglia and upregulated proinflammatory cytokines. Expressions of chemokines CXCL7, CX3CL1, and CCL2 were altered. The causal link of chemokines to PVN neuroinflammation and hypertension remain to be determined.

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Increased CCL24 and CXCL7 levels in the cerebrospinal fluid of patients with neurosyphilis

Cited by 10 publications

References 37 publications

PPBP gene as a biomarker for coronary heart disease risk in postmenopausal Thai women

PPBP gene as a biomarker for coronary heart disease risk in postmenopausal Thai women

Identifying platelet-derived factors as amplifiers ofB. burgdorferi-induced cytokine production

Upregulation of Chemokines in the Paraventricular Nucleus of the Hypothalamus in Rats with Stress-Induced Hypertension

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