Increased Cell-free Fetal DNA in Plasma of Two Women with Invasive Placenta

Sekizawa, Akihiko; Jimbo, Masatoshi; Saitô, Hiroshi; Iwasaki, Mariko; Sugito, Yumi; Yukimoto, Yasuo; Otsuka, Junko; Okai, Takashi

doi:10.1093/clinchem/48.2.353

Cited by 119 publications

(53 citation statements)

References 8 publications

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“…Sekizawa et al reported that the level of cff DNA was higher in placenta accreta. 29 Furthermore, cff mRNA was also detected in maternal serum. 30 Recently, it was reported that circulating miR-34a could be an indicator of the progression of various diseases, such as diabetes and hepatitis.…”

Section: Discussionmentioning

confidence: 96%

Roles of microRNA‐34a in the pathogenesis of placenta accreta

Umemura

Ishioka

Endo

et al. 2012

J of Obstet and Gynaecol

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Section: Discussionmentioning

confidence: 96%

Roles of microRNA‐34a in the pathogenesis of placenta accreta

Umemura

Ishioka

Endo

et al. 2012

J of Obstet and Gynaecol

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“…This finding suggests that antenatal detection of invasive placenta might be achievable through analysis of fetal DNA concentrations in maternal plasma. 57 Furthermore, we have also reported on a case of placenta increta, in which a small part of the placenta remained adherent despite manual removal of the placenta at the time of delivery. The patient was followed up by monitoring plasma concentrations of beta-human chorionic gonadotrophin (b-hCG) and fetal DNA levels after delivery.…”

Section: Invasive Placenta (Abnormal Adherence Of the Placenta)mentioning

confidence: 94%

“…Furthermore, as mentioned above, we have previously identified elevated concentrations of fetal DNA within the maternal plasma of women with placenta previa, particularly in those with invasive placenta. 57,58 In these cases, trophoblasts invading the myometrium are attacked and destroyed by the maternal immune system, resulting in increased levels of fetal DNA in maternal plasma. Furthermore, fetal DNA concentrations in maternal plasma increase as pregnancies advance, 16,75 and placental apoptosis increases with gestational age.…”

Section: Origin Of Fetal Dna In Maternal Plasma or Serummentioning

confidence: 99%

Recent advances in non‐invasive prenatal DNA diagnosis through analysis of maternal blood

Sekizawa¹,

Purwosunu

Matsuoka

et al. 2007

J of Obstet and Gynaecol

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Prenatal diagnosis of aneuploidy and single-gene disorders is usually performed by collecting fetal samples through amniocentesis or chorionic villus sampling. However, these invasive procedures are associated with some degree of risk to the fetus and/or mother. Therefore, in recent years, considerable effort has been made to develop non-invasive prenatal diagnostic procedures. One potential non-invasive approach involves analysis of cell-free fetal DNA in maternal plasma or serum. Another approach utilizes fetal cells within the maternal circulation as a source of fetal DNA. At the present time, fetal gender and fetal RhD blood type within RhD-negative pregnant women can be reliably determined through analysis of maternal plasma. Furthermore, genetic alterations can be diagnosed in the maternal plasma when the mother does not have the alterations. However, the diagnosis of maternally inherited genetic disease and aneuploidy is limited using this approach. Non-invasive prenatal diagnosis through examination of intact fetal cells circulating within maternal blood can be used to diagnose a full range of genetic disorders. Since only a limited number of fetal cells circulate within maternal blood, procedures to enrich the cells and enable single cell analysis with high sensitivity are required. Recently, separation methods, including a lectin-based method and autoimage analyzing, have been developed, which have improved the sensitivity of genetic analysis. This progress has supported the possibility of non-invasive prenatal diagnosis of genetic disorders. In the present article, we discuss recent advances in the field of non-invasive prenatal diagnosis.

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“…[31][32][33] Increased cellfree fetal DNA level has also been observed in certain aneuploidies 34,35 and other pregnancy-associated complications. [36][37][38][39][40] The clinical utility of these findings has not yet been widely used, possibly due to two reasons: (a) biologically, the cell-free fetal DNA levels in normal and abnormal pregnancies overlap significantly; (b) quantification inaccuracy causes the overlap of cell-free fetal DNA levels in different pregnancies. It is quite common that only 10 to 20 copies of fetal template are present prior to PCR.…”

Section: Quantitative Analysismentioning

confidence: 99%

MALDI‐TOF Mass Spectrometry for Quantitative, Specific, and Sensitive Analysis of DNA and RNA

Ding

2006

Annals of the New York Academy of Sciences

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Cell-free fetal DNA and RNA released into the maternal circulation offer new opportunities to study fetal and pregnancy-associated abnormalities. Similarly, tumor cells can release cell-free DNA and RNA into the peripheral circulation, and these cell-free DNA and RNA can be used for cancer diagnosis, monitoring, and prognosis. However, these DNA and RNA often exist at very low concentrations (for fetal DNA, approximately 20 genome-equivalents (G.E.)/mL of plasma in the first trimester). The analysis is further complicated by the predominant amount of blood cell-derived DNA and RNA. MALDI-TOF mass spectrometry can provide quantitative, specific, and sensitive analysis of DNA and RNA, and thus may be a useful technology for the field.

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Increased Cell-free Fetal DNA in Plasma of Two Women with Invasive Placenta

Cited by 119 publications

References 8 publications

Roles of microRNA‐34a in the pathogenesis of placenta accreta

Roles of microRNA‐34a in the pathogenesis of placenta accreta

Recent advances in non‐invasive prenatal DNA diagnosis through analysis of maternal blood

MALDI‐TOF Mass Spectrometry for Quantitative, Specific, and Sensitive Analysis of DNA and RNA

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