Increased collagenase activity in macrophages from bronchial lavage as a diagnostic marker of non-small cell lung cancer

Hakoda, Yusuke; Nagate, Akira; Minemura, Kazushige; Utsumi, Kazuhiko R.; Aoshima, Masahiro; Ohyashiki, Kazuma

doi:10.1136/thorax.58.2.122

Cited by 5 publications

(2 citation statements)

References 26 publications

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“…Though macrophages are considered potential diagnostic tools for pulmonary disease, their clinical relevance may vary among individuals [ 162 , 163 ]. Nevertheless, as the “gatekeepers” of the respiratory system, the mechanisms of macrophages cannot be overlooked.…”

Section: Targeting Llms For the Treatment Of Treat Lung Diseasesmentioning

confidence: 99%

Lipid-Laden Macrophages in Pulmonary Diseases

Zhu,

Choi,

Somanath

et al. 2024

Cells

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Pulmonary surfactants play a crucial role in managing lung lipid metabolism, and dysregulation of this process is evident in various lung diseases. Alternations in lipid metabolism lead to pulmonary surfactant damage, resulting in hyperlipidemia in response to lung injury. Lung macrophages are responsible for recycling damaged lipid droplets to maintain lipid homeostasis. The inflammatory response triggered by external stimuli such as cigarette smoke, bleomycin, and bacteria can interfere with this process, resulting in the formation of lipid-laden macrophages (LLMs), also known as foamy macrophages. Recent studies have highlighted the potential significance of LLM formation in a range of pulmonary diseases. Furthermore, growing evidence suggests that LLMs are present in patients suffering from various pulmonary conditions. In this review, we summarize the essential metabolic and signaling pathways driving the LLM formation in chronic obstructive pulmonary disease, pulmonary fibrosis, tuberculosis, and acute lung injury.

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Section: Targeting Llms For the Treatment Of Treat Lung Diseasesmentioning

confidence: 99%

Lipid-Laden Macrophages in Pulmonary Diseases

Zhu,

Choi,

Somanath

et al. 2024

Cells

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“…The presence of collagenase and a small number of macrophages could be explained by healthy tissues surrounding copolymer hydrogels because of the antimicrobial activity of CF. 59,60…”

Section: Groupsmentioning

confidence: 99%

pH-Responsive 2-hydroxyethyl methacrylate/citraconic anhydride–modified collagen hydrogels as ciprofloxacin carriers for wound dressings

Pamfil

Vasile

Mititelu-Tarțău

et al. 2017

Journal of Bioactive and Compatible Polymers

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pH-Sensitive hydrogels of 2-hydroxyethyl methacrylate/citraconic anhydride–modified collagen were obtained by free radical copolymerization/crosslinking in the presence of ammonium persulfate/N,N,N′,N′-tetramethylethylenediamine redox initiator system. Their pH-responsiveness was demonstrated by swelling behavior and ciprofloxacin release tests. Both unloaded and loaded hydrogels were characterized by Fourier transform infrared, scanning electron microscopy, and biocompatibility tests. The enzymatic degradation in the presence of Clostridium histolyticum mainly depends on initiator content. In vivo biocompatibility tests involving intraperitoneal hydrogels’ implantation in rats following the analysis by the granuloma test, leukocyte formula, immune parameters, and hepatic transaminases demonstrated their non-toxicity and biocompatibility with living tissues. The in vitro antimicrobial activity, in vivo biocompatibility, and in vitro biodegradability tests attest the possibility to use these new polymeric hydrogels with tailored properties as matrices for bioactive products in medical and pharmaceutical applications as wound care and targeting drug delivery systems. The ciprofloxacin release studies proved their potential as materials for wound dressings.

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Increased intracellular activity of matrix metalloproteinases in neutrophils may be associated with delayed healing of infection without neutropenia in myelodysplastic syndromes

Yamaguchi

Ohyashiki

2005

Ann Hematol

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To investigate the influence of the intracellular activity of type II and type IV collagenases [matrix metalloproteinases (MMP)-2, MMP-8, and MMP-9] in neutrophils from patients with myelodysplastic syndromes (MDS), we tried to measure intracellular activity using flow cytometric techniques. We also studied the clinical features of patients showing high activity. The intracellular collagenase activity, expressed as a ratio to the standardized fluorescence intensity, in patients with MDS was significantly higher than normal volunteers (19.5+/-14.8 vs 13.3+/-6.8, p=0.024). The difference among subcategories of MDS according to the French-American-British (FAB) and WHO classifications was not significant. No significant influence of three variables of the International Prognostic Scoring System (IPSS) was seen on activity. Of 8 patients with activity of more than 26.9 (mean+2 standard deviations of normal controls), 5 experienced an episode of delayed healing of infection without neutropenia, while 1 of 43 patients with activity of less than 26.9 experienced such an episode (p=0.0002). The average collagenase activity of six patients with delayed healing of infection without neutropenia (44.7+/-28.9) was significantly higher than that of other MDS patients (16.0+/-7.1, p=0.005) (Fig. 4). It was also significantly higher than the activity of the control group (13.3+/-6.8, p=0.011). Our report suggests that increased collagenase activity in neutrophils may delay healing of infection. In addition, we suggest that increased collagenase activity may be an independent prognostic factor for the susceptibility to severe infection in MDS.

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Increased collagenase activity in macrophages from bronchial lavage as a diagnostic marker of non-small cell lung cancer

Cited by 5 publications

References 26 publications

Lipid-Laden Macrophages in Pulmonary Diseases

Lipid-Laden Macrophages in Pulmonary Diseases

pH-Responsive 2-hydroxyethyl methacrylate/citraconic anhydride–modified collagen hydrogels as ciprofloxacin carriers for wound dressings

Increased intracellular activity of matrix metalloproteinases in neutrophils may be associated with delayed healing of infection without neutropenia in myelodysplastic syndromes

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