Increased Dialysate Levels of Phospholipids Containing Unsaturated Fatty Acid Are Associated with Increased Peritoneal Transport Rate

Lipid profiling of rat peritoneal surface layers by online normal- and reversed-phase 2D LC QToF-MS

“…Previous surfaceactive phospholipid research in peritoneal dialysis ( 46 ) was focused on the correlation between PC and peritoneal function, such as changes of PC in peritoneal surface or dialysate and recovery of destroyed peritoneum by PC replenishment through intraperitoneal, intravenous, and oral administration. Combined with our research, there are two reasons for the focus: 1 ) PC was the major class both in rat peritoneal surface ( 33 ) and dialysate ( 47 ), which means even a small proportion of PC altered by some stimulation could be enough to be detected. It is reasonable that these changes are not crucial but detectable by traditional analytical approaches; and 2 ) previous detection methods, such as TLC and 1D LC-MS ( 5,33,47 ), cannot determine LpPL molecular species, let alone the discovery of their changes.…”

“…Combined with our research, there are two reasons for the focus: 1 ) PC was the major class both in rat peritoneal surface ( 33 ) and dialysate ( 47 ), which means even a small proportion of PC altered by some stimulation could be enough to be detected. It is reasonable that these changes are not crucial but detectable by traditional analytical approaches; and 2 ) previous detection methods, such as TLC and 1D LC-MS ( 5,33,47 ), cannot determine LpPL molecular species, let alone the discovery of their changes. In our study, signifi cant PC differences have not been observed between the control and dosed groups, but the LpPL species was observed to be dramatically increased in rat peritoneal Supplemental Material can be found at:…”