Increased expression of a high molecular weight matrix component in rat hepatocellular carcinoma

Abstract

The urea extract of the glycoproteins from the extracellular matrix of rat liver has been compared with that of Morris hepatoma 7777. A high molecular weight glycoprotein present in Morris hepatoma 7777 was not found in the extract of liver matrix. Under reducing conditions in SDS-gel electrophoresis this component gave two glycoprotein bands with Mr 53 k and 56 k. The indirect immunofluorescence staining with a monospecific antiserum directed against the component showed its abundant presence in Morris hepatoma 7777 as well as in the less malignant Morris hepatoma 9121 in form of extracellular network structures. The antigen also densely filled some cumuli of cells. In contrast the liver tissue showed only very weak staining of the extracellular areas. The overall distribution of the component could be correlated with the distribution of several hydrolases in the tumor matrix, notably beta-D-glucuronidase.

The endothelin system in Morris hepatoma‐7777: an endothelin receptor antagonist inhibits growth in vitro and in vivo

“…Tumor mince (1 ml) was diluted with 0.5 ml 0.9% NaCl and 0.5 ml of the resulting suspension was injected using a 20‐g needle. This tumor model is well established in our laboratory ( Riese et al , 1987 ).…”

“…MH‐7777 cell suspensions were injected into both hindlimbs of 44 male Buffalo rats ( Riese et al , 1987 ). The rats were randomly divided into two groups with 22 rats.…”

Immunological Screening of a Glycoprotein Antigen Expressed by Zajdela Ascites Hepatoma Cells on Normal Rat Tissues and Tumour Cells

“…Morris hcpatoma 7777 was a rapidly proliferaling. poorly differentiated hoputocellular carcinoma [22]. Faza hepatoma [15] was a reverianl cell line derived from a de-differentiated variant of Reuber H 35 ral hepaloma [23|.…”