Increased expression of the mRNA for hormone-sensitive lipase in adipose tissue of cancer patients

Thompson, Matthew; Cooper, Sandra T.; Parry, Bryan R.; Tuckey, John

doi:10.1016/0925-4439(93)90044-2

Cited by 84 publications

(50 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…LIPE is an adipocyte enzyme that cleaves fatty acids from intracellular triacylglycerol (Mersmann, 1998), and an increase in LIPE could depress the accumulation of triglycerol in adipose cells (Thompson et al, 1993;Sztalryd et al, 1995). In the present study, there was no obvious relationship between LIPE expression and carcass fat content in the two breeds, but there was a significant positive relationship between carcass fat content and the ratio of FASN to LIPE mRNA.…”

Section: Discussioncontrasting

confidence: 57%

Developmental patterns of FASN and LIPE mRNA expression in adipose tissue of growing Jinhua and Landrace gilts

Miao¹,

Zhu²,

Zhang³

et al. 2010

Czech J. Anim. Sci.

View full text Add to dashboard Cite

ABSTRACT:The present study was aimed to investigate the developmental patterns of FASN (fatty acid synthase) and LIPE (lipase, hormone-sensitive) mRNA in adipose tissue in pigs of different breeds and the relation with carcass fat content. Subcutaneous adipose tissue was sampled and total RNA was extracted to determine FASN and LIPE mRNA levels by semi-quantitative RT-PCR. The results showed that the FASN mRNA level increased with age (P < 0.05) and Jinhua gilts expressed higher FASN mRNA compared with Landrace gilts at 80 and 125 days of age (P < 0.05). In addition, Jinhua gilts expressed lower LIPE mRNA compared with Landrace gilts at 80 days of age (P < 0.01). Furthermore, the ratio of FASN/LIPE mRNA had a similar model in the two breeds, and was higher in Jinhua gilts than that in Landrace gilts at 80 and 125 days of age (P < 0.05). The FASN mRNA level was positively related to carcass fat content in Jinhua and Landrace gilts (r = 0.802, P = 0.01; r = 0.734, P = 0.02; respectively), and the ratio of FASN/LIPE expression exhibited significantly positively related carcass fat content (r = 0.804, P = 0.01; r = 0.749, P = 0.02; respectively).

show abstract

Section: Discussioncontrasting

confidence: 57%

Developmental patterns of FASN and LIPE mRNA expression in adipose tissue of growing Jinhua and Landrace gilts

Miao¹,

Zhu²,

Zhang³

et al. 2010

Czech J. Anim. Sci.

View full text Add to dashboard Cite

show abstract

“…Although the defects in adipocyte development and metabolism are implicated in the pathogenesis of HIV-related lipodystrophy (Bastard et al, 2002), little is known the mechanism underlying malignancy-related lipoatrophy. Lipolysis appears to be increased in cancer cachexia since in white fat lipoprotein lipase activity is decreased in tumour-bearing mice (Thompson et al, 1981) while hormone sensitive lipase mRNA is elevated in cancer patients with cachexia (Thompson et al, 1993). In addition, hyperlipidaemia is often present in cachectic cancer patients (Rofe et al, 1994) and in tumour-bearing rats (Lopez-Soriano et al, 1997a).…”

mentioning

confidence: 99%

Adipose atrophy in cancer cachexia: morphologic and molecular analysis of adipose tissue in tumour-bearing mice

et al. 2006

View full text Add to dashboard Cite

Extensive loss of adipose tissue is a hallmark of cancer cachexia but the cellular and molecular basis remains unclear. This study has examined morphologic and molecular characteristics of white adipose tissue in mice bearing a cachexia-inducing tumour, MAC16. Adipose tissue from tumour-bearing mice contained shrunken adipocytes that were heterogeneous in size. Increased fibrosis was evident by strong collagen-fibril staining in the tissue matrix. Ultrastructure of 'slimmed' adipocytes revealed severe delipidation and modifications in cell membrane conformation. There were major reductions in mRNA levels of adipogenic transcription factors including CCAAT/enhancer binding protein alpha (C/EBPa), CCAAT/enhancer binding protein beta, peroxisome proliferatoractivated receptor gamma, and sterol regulatory element binding protein-1c (SREBP-1c) in adipose tissue, which was accompanied by reduced protein content of C/EBPa and SREBP-1. mRNA levels of SREBP-1c targets, fatty acid synthase, acetyl CoA carboxylase, stearoyl CoA desaturase 1 and glycerol-3-phosphate acyl transferase, also fell as did glucose transporter-4 and leptin. In contrast, mRNA levels of peroxisome proliferators-activated receptor gamma coactivator-1alpha and uncoupling protein-2 were increased in white fat of tumour-bearing mice. These results suggest that the tumour-induced impairment in the formation and lipid storing capacity of adipose tissue occurs in mice with cancer cachexia.

show abstract

“…LMF was also found to stimulate expression of HSL in 3T3 adipocytes, which may explain the increased levels observed in adipose tissue of cancer patients (Thompson et al, 1993).…”

Section: Discussionmentioning

confidence: 90%

“…The elevation of cyclic AMP would lead to an activation of hormone-sensitive lipase (HSL). Thompson et al (1993) observed a 2-fold elevation in serum triacylglycerol and fatty acid levels in cancer patients compared with normal controls, and a 2-fold increase in the relative level of mRNA for HSL in adipose tissue. There was no change in the relative level of the mRNA for lipoprotein lipase (LPL), suggesting that an increased rate of mobilization, rather than deposition, was most important for the depletion of lipid stores.…”

mentioning

confidence: 88%

Modulation of adipocyte G-protein expression in cancer cachexia by a lipid-mobilizing factor (LMF)

et al. 2001

View full text Add to dashboard Cite

Summary Adipocytes isolated from cachectic mice bearing the MAC 16 tumour showed over a 3-fold increase in lipolytic response to both low concentrations of isoprenaline and a tumour-derived lipid mobilizing factor (LMF). This was reflected by an enhanced stimulation of adenylate cyclase in plasma membrane fractions of adipocytes in the presence of both factors. There was no up-regulation of adenylate cyclase in response to forskolin, suggesting that the effect arose from a change in receptor number or G-protein expression. Immunoblotting of adipocyte membranes from mice bearing the MAC16 tumour showed an increased expression of Gαs up to 10% weight loss and a reciprocal decrease in Gα. There was also an increased expression of Gαs and a decrease in Gα in adipose tissue from a patient with cancer-associated weight loss compared with a non-cachectic cancer patient. The changes in G-protein expression were also seen in adipose tissue of normal mice administered pure LMF as well as in 3T3L1 adipocytes in vitro. The changes in G-protein expression induced by LMF were attenuated by the polyunsaturated fatty acid, eicosapentaenoic acid (EPA). This suggests that this tumour-derived lipolytic factor acts to sensitize adipose tissue to lipolytic stimuli, and that this effect is attenuated by EPA, which is known to preserve adipose tissue in cancer cachexia.

show abstract

Increased expression of the mRNA for hormone-sensitive lipase in adipose tissue of cancer patients

Cited by 84 publications

References 39 publications

Developmental patterns of FASN and LIPE mRNA expression in adipose tissue of growing Jinhua and Landrace gilts

Developmental patterns of FASN and LIPE mRNA expression in adipose tissue of growing Jinhua and Landrace gilts

Adipose atrophy in cancer cachexia: morphologic and molecular analysis of adipose tissue in tumour-bearing mice

Modulation of adipocyte G-protein expression in cancer cachexia by a lipid-mobilizing factor (LMF)

Contact Info

Product

Resources

About