Increased Isolation and Characterization of <i>Shigella sonnei</i> Obtained from Hospitalized Children in Tehran, Iran

Abstract

Shigella flexneri has been the most frequent cause of shigellosis in children in Iran. To evaluate the changes in frequency of serogroups, 302 Shigella species were isolated in 2003 from hospitalized children, aged less than 12 years, with acute diarrhoea in Tehran, Iran. The number of collected S. sonnei, S. flexneri, S. boydii, and S. dysenteriae isolates was 178 (58.9%), 110 (37.4%), 10 (3.3%), and 4 (1.3%) respectively. Most (94%) S. sonnei isolates were resistant to co-trimoxazole. They were, however, relatively or completely sensitive to 15 commonly-used antibiotics. The extracted plasmids showed 12 different profiles with two closelyrelated patterns constituting 70% of the total isolates. Ribotyping, using PvuII, HindIII or SalI restriction enzymes, generated a single pattern for all S. sonnei isolates. Data suggest that S. sonnei has become the predominant serogroup in children in the hospitals of Tehran.

Ribotyping as a Molecular Biological Technique for Studying Diversity in <i>Shigella</i> Isolates - A Review

“…In a recent study all S. boydii strains were categorized into two patterns only. Moreover, all but one isolates, belonged to ribotype I (Ranjbar et al, 2008). However, this finding is similar to recent reports from other developing countries, such as Bangladesh (Talukder et al, 2006b).…”

“…Nastasi and colleagues have also found 13 ribotypes by examining 432 S. sonnei isolates (Nastasi et al, 1993). Ranjbar et al (Ranjbar et al, 2008) observed a single ribotype pattern among the S. sonnei isolates collected in Tehran.…”

Clonal dissemination of a single Shigella sonnei strain among Iranian children during Fall 2012 in Tehran, I.R. Iran

“…Recently, other reports have also indicated the increasing resistance to streptomycin and cotrimoxazole among Shigella spp. in Iran and other countries (Ranjbar et al, 2008;Seol et al, 2006;Vrints et al, 2009). …”

“…in developing countries while in Europe and North America S. sonnei is predominant and is a reflection of standards of living (1994). In the last decades several reports have revealed that the majority of shigellosis in Iran is caused by S. flexneri but in recent years a growing tendency has been observed toward S. sonnei (Farshad et al, 2006;Ranjbar et al, 2008). Increasing incidence of S. sonnei versus S. flexneri may imitate the industrialization of the capital city and the improving hygiene level in Iran (Ranjbar et al, 2008).…”

Global Phylogeny of Shigella sonnei Strains from Limited Single Nucleotide Polymorphisms (SNPs) and Development of a Rapid and Cost-Effective SNP-Typing Scheme for Strain Identification by High-Resolution Melting Analysis

“…higella sonnei has emerged as a major global cause of shigellosis (1)(2)(3)(4)(5). There are 4 distinct S. sonnei lineages, and the current global burden involves several geographically associated, multidrug-resistant clones that belong to a single lineage (1).…”