Increased Level of Antibodies Cross-Reacting with Ves v 5 and CRISP-2 in MAR-Positive Patients

Brunner-Agten, Saskia; Pavlovic, Rodoljub; Müller, Lorenz; Horn, Michael P.; Huber, Andreas; Stadler, Beda M.; Vogel, Monique

doi:10.1159/000338946

Cited by 10 publications

(6 citation statements)

References 46 publications

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“…These autoantibodies lead to sperm immobilization and bind along the sperm surface, including the head, where CRISP-2 is localized (Brunner-Agten et al 2013). Additionally, the fact that these autoantibodies can bind to Ves V 5 led the authors to hypothesize that ASA-positive men may have a higher risk to develop wasp allergy (Brunner-Agten et al 2013). However, ASA positivity in this study was considered above 10% which is very different from the WHO reference value.…”

Section: Autoimmune Diseasescontrasting

confidence: 54%

“…The authors demonstrated that ASA-positive men had a higher incidence of allergy against Hymenoptera venom, and the results also showed a high amount of autoantibodies that cross-react with both CRISP-2 and Ves V 5 allergen. These autoantibodies lead to sperm immobilization and bind along the sperm surface, including the head, where CRISP-2 is localized (Brunner-Agten et al 2013). Additionally, the fact that these autoantibodies can bind to Ves V 5 led the authors to hypothesize that ASA-positive men may have a higher risk to develop wasp allergy (Brunner-Agten et al 2013).…”

Section: Autoimmune Diseasesmentioning

confidence: 99%

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The impact of antisperm antibodies on human male reproductive function: an update

2021

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Immune infertility occurs due to the presence of antisperm antibodies (ASA). This type of infertility has a relatively low prevalence (2.6-6.6% in infertile men), and its etiology, risk factors, targets and consequences for male fertility are not completely understood. While it is largely accepted that abnormalities in the blood-testis barrier and/or blood-epididymal barrier are the main factors behind its etiology, and that sperm motility is the most frequently reported altered parameter, few are the well-defined risk factors and ASA targets only now started to be disclosed, with proteins involved in sperm-oocyte interaction rising as the most significant. The development of potential treatments is also limited, being the corticosteroids the more promising. Overall, there are still many knowledge gaps related to immune infertility. With this review we aimed to gather all the information collected from studies developed in humans in the last decade. Despite the controversial results/inconsistencies, that are not only a result from the complexity of mechanisms/variables involved in ASA infertility, but also from the technical approaches to assess ASA and the lack of a consensus regarding the thresholds to be used, this manuscript aims to bring a fresh update on the field. It has become clear that, to obtain more/reliable data, there is a need to assess ASA in all the routine seminal analysis, following WHO recommendations. In this way it will be possible to obtain consistent and comparable information, that can add to current knowledge. Additionally, multicentric studies with large cohorts are also missing, and future research should take this into consideration.

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Section: Autoimmune Diseasescontrasting

confidence: 54%

Section: Autoimmune Diseasesmentioning

confidence: 99%

The impact of antisperm antibodies on human male reproductive function: an update

2021

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“…On the other hand, Fu et al 22 recently developed an enzyme‐linked immunosorbent assay (ELISA) using anti‐actin‐like protein (ACTL)7a antibodies because they thought that previously available tests of ASA detection showed only the binding of antibodies on the sperm surface, but no antigenic information could be applied 23‐25 . They have focused on the sperm‐specific expression protein ACTL7a and found that ACTL7a could be recognized by the antibodies contained in infertile serum, which could reduce mouse fertility rates to zero 26 .…”

Section: Detection Of Anti‐sperm Antibodiesmentioning

confidence: 99%

Anti‐sperm antibodies and reproductive failures

Shibahara

Wakimoto

Fukui

et al. 2020

American J Rep Immunol

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Reproductive failures include infertility and recurrent pregnancy loss (RPL). Although the relative importance of immunological factors in human reproduction remains unclear, there may be immune‐mediated reproductive failures in a portion of unexplained infertility and RPL. As a cause of immunological factor, anti‐sperm antibodies (ASA) are produced both in men and women. There have been reported several antigens in the surface of sperm that are especially foreign to women. The presence of ASA, especially sperm‐immobilizing antibodies, in the sera of infertile women has been shown to inhibit sperm migration in the female genital tract. Therefore, the effectiveness of the treatments for infertile women with sperm‐immobilizing antibodies by timed intercourse or intra‐uterine insemination is limited. Such antibodies can also exert inhibitory effects on various stages of sperm‐egg interaction and subsequent embryo development in vitro. It is suggested that ASA testing for infertile women should be performed before proceeding IVF. The manipulation of gametes and embryos from patients having sperm‐immobilizing antibodies should be carefully carried out especially to avoid contaminating patient's serum and follicular fluid in the culture medium in order to overcome the immunological causes of female infertility by ASA, and satisfactory results under suitable conditions for gametes and embryos have been obtained. The relationship between ASA and RPL was controversially reported. Increased miscarriage rates in women with ASA were demonstrated by some authors. In contrast, lack of association between ASA and RPL was reported. In this manuscript, we are focusing the roles of ASA in women with reproductive failures.

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“…Several tests are available to detect ASA in either semen or serum, including ‘direct’ tests for antibodies bound to the sperm and ‘indirect’ tests in sperm-free fluids such as serum. Previously available tests of ASA detection showed only the binding of antibodies on the sperm surface, but no antigenic information could be applied 10 11 12 . Antibodies derived from accurate, specific spermatozoa antigens should be detected and assessed for possible use in clinical diagnosis.…”

mentioning

confidence: 99%

Immune Infertility Should Be Positively Diagnosed Using an Accurate Method by Monitoring the Level of Anti-ACTL7a Antibody

Yao

Luo

et al. 2016

Sci Rep

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Infertility is currently a major public health problem. Anti-sperm antibodies (ASAs) markedly reduce sperm quality, which can subsequently lead to male and/or female infertility. The accurate detection of ASAs derived from specific spermatozoa is, therefore, clinically useful. We have focused on the spermatozoa-specific expression protein ACTL7a for many years and have developed an enzyme-linked immunosorbent assay (ELISA) to detect the concentration of anti-ACTL7a antibodies in fertile sera (n = 267) and infertile sera (n = 193). Infertile sera were collected from the positive sera of tray agglutination tests (TAT), which is a routine ASA screening methodology. We found that the concentration of anti-ACTL7a antibodies was significantly higher in the infertile sera (than in the fertile sera, P < 0.0001) and much higher in the TAT ≥ 16 infertile sera. The ELISA was much better for male sera detection (AUC = 0.9899). If we set the standard at a strongly positive value (calculated by ROC curve), the positive predictive value of the antibody detection reached 100 percent, with a false positive rate of zero. The developed ELISA method for anti-ACTL7a antibody detection is therefore sensitive, accurate, and easy to perform, making it an excellent potential tool for future clinical use.

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Increased Level of Antibodies Cross-Reacting with Ves v 5 and CRISP-2 in MAR-Positive Patients

Cited by 10 publications

References 46 publications

The impact of antisperm antibodies on human male reproductive function: an update

The impact of antisperm antibodies on human male reproductive function: an update

Anti‐sperm antibodies and reproductive failures

Immune Infertility Should Be Positively Diagnosed Using an Accurate Method by Monitoring the Level of Anti-ACTL7a Antibody

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