Increased levels of angiopoietins 1 and 2 in sputum supernatant in severe refractory asthma

Tseliou, Eleni; Bakakos, Petros; Κostikas, Κonstantinos; Hillas, Georgios; Mantzouranis, Konstantinos; Emmanouil, Philip; Simoes, Davina Camargo Madeira; Alchanatis, Manos; Papiris, Spyros; Loukides, Stelios

doi:10.1111/j.1398-9995.2011.02768.x

Cited by 33 publications

(24 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These findings are in accordance with recent literature (20)(21)(22)(23)(24), although, to the best of our know ledge, this is the first study to report mRNA expression levels of both VEGF and its receptor FLT-1 in human nasal polyp tissue. Mounting evidence suggests that, besides its influence on blood vessel formation, VEGF may even be directly involved in the pathogenesis of bronchial asthma (30,31). Similarly, we hypothesized that VEGF may mediate upper airway remodeling observed in the development of sinonasal polyposis.…”

Section: Discussionmentioning

confidence: 99%

“…In a recent study on severe asthma, the two angiopoietins levels were higher compared to the healthy subjects. In severe asthma, Ang-2 is associated with mediators involved in both the inflammatory and the vascular remodeling processes (31). Ang-1, however, has been shown to induce pro-inflammatory effects, such as eosinophil chemotaxis, and also contributes to the increased secretion of matrix metalloproteinase-2 as well as the decreased secretion of tissue inhibitors of metalloproteinase-2 (33).…”

Section: Discussionmentioning

confidence: 99%

“…Th2 cells, a key element in bronchial asthma pathophysiology, have been shown to express receptors for VEGF. Moreover, evidence exists showing that high VEGF levels may be directly associated with asthma severity, as they correlate directly with disease activity and inversely with airway caliber and airway responsiveness (30,31). VEGF has been shown not only to promote cell growth, but also to inhibit apoptosis in epithelial cells and to auto-regulate its own production in a positive manner in non-neoplastic primary human airway epithelial cells.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Investigation of angiogenetic pathways in nasal polyposis

et al. 2012

View full text Add to dashboard Cite

Abstract. Tissue angiogenesis is a complex phenomenon that results in the growth of new blood vessels from the microcirculation. This process has been known to play a crucial role in tumor growth as well as several benign diseases. The aim of this study was to assess mRNA expression of various angiogenic factors and chemokines in nasal polyps and compare the results to normal nasal mucosa. mRNA expression was measured using real-time RT-PCR for the following angiogenic factors and chemokines: VEGF, VEGFR-1, Ang-1, Ang-2, Tie-2A, Tie-2B, SDF-1α, SDF-1β, CXCR4 and YY1. Biopsy specimens from nasal polyps in the polyposis group and middle turbinates in the control group were studied. A total of 18 nasal polyposis patients were studied and compared to 10 control subjects. Results showed VEGF, VEGFR-1, Ang-1, Ang-2, Tie-2A, Tie-2B, SDF-1α and SDF-1β mRNA expression to be significantly higher in nasal polyposis patients compared to the control group (p<0.05). The findings of this study support the role of angiogenic growth factors in the pathogenenesis of nasal polyposis. Further studies are required to confirm these results and evaluate potential clinical implications. IntroductionNasal polyposis (NP) constitutes a chronic inflammatory disease of the nose and sinuses characterized by the appearance of protruding tissue swellings of the nasal mucosa. These swellings most commonly originate from the middle meatus and anterior ethmoids. On histologic examination, they are typically characterized by proliferation of the epithelial layer, glandular hyperplasia, thickening of the basal membrane, focal fibrosis, edema and cellular infiltration of the stromal layer. Nasal polyps are relatively common in the general population and significantly affect patient quality of life. Despite these facts, etiology of the disease remains largely unknown (1-4).Tissue angiogenesis is a complex phenomenon that results in the growth of new blood vessels from the microcirculation (5-7). This process has been known to play a crucial role in tumor growth, where it is regarded as a prerequisite allowing for the expansion of tumor populations beyond the size restricted by oxygen and nutrient diffusion (6). It also facilitates tumor progression and metastasis by causing degradation of the epithelial basement membrane and providing cancer cells with new capillaries for entering the circulation (7,8).Increasing evidence shows that angiogenesis may also play a critical role in benign disorders (9,10). The phenomenon, appearing in the form of growth of new blood vessels or the enlargement of existing ones, has been strongly associated with airway remode ling in chronic asthma and chronic obstructive pulmonary disease (11,12). In addition, using immunohistochemistry it has been shown that vascular endothelial growth factor (VEGF), possibly the most important driver of angiogenesis, along with its receptors, are strongly expressed in nasal polyp tissues, thus supporting a role in the development of chronic inflammation and edema in this disease (13).Re...

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Investigation of angiogenetic pathways in nasal polyposis

et al. 2012

View full text Add to dashboard Cite

show abstract

“…Sputum induction was performed as previously described [12,13], using all the modifications for safe measurements according to the underlying asthma severity [14,15]. The process had a total duration of 15 min, where subjects inhaled 3% saline at room temperature by an ultrasonic nebulizer (DeVilbiss Co., Heston, UK).…”

Section: Sputum Induction and Processingmentioning

confidence: 99%

Persistent airflow obstruction in patients with asthma: Characteristics of a distinct clinical phenotype

Konstantellou

Papaioannou

Loukides

et al. 2015

Respiratory Medicine

View full text Add to dashboard Cite

show abstract

“…dexamethasone and A3R activation might underlie the resistance to corticosteroids that is experienced in severe refractory asthma. Consistent with this notion is the tight association between severe refractory asthma and intense inflammatory fibrosis that is linked with increased levels of angiopoietin, VEGF, IL8 and SPP1 in the sputum supernatant (Simpson et al, 2008;Delimpoura et al, 2010;Tseliou et al, 2012;Hantera et al, 2014).…”

Section: Discussionmentioning

confidence: 56%

Down-regulation of the A3 adenosine receptor in human mast cells upregulates mediators of angiogenesis and remodeling

Rudich

Dekel

Sagi‐Eisenberg

2015

Molecular Immunology

View full text Add to dashboard Cite

Increased levels of angiopoietins 1 and 2 in sputum supernatant in severe refractory asthma

Abstract: Our results indicate that both angiopoietins levels are higher in SRA compared with moderate asthma and healthy subjects. In SRA, Ang-2 is associated with mediators involved in both the inflammatory and the vascular remodeling processes.

Cited by 33 publications

References 26 publications

Investigation of angiogenetic pathways in nasal polyposis

Investigation of angiogenetic pathways in nasal polyposis

Persistent airflow obstruction in patients with asthma: Characteristics of a distinct clinical phenotype

Down-regulation of the A3 adenosine receptor in human mast cells upregulates mediators of angiogenesis and remodeling

Contact Info

Product

Resources

About