Increased Levels of Lipid Peroxidation Products Malondialdehyde and 4-Hydroxynonenal after Perinatal Hypoxia

Schmidt, Heike; Grune, Tilman; Müller, Rainer H.; Siems, Werner; Wauer, Roland R.

doi:10.1203/00006450-199607000-00003

Cited by 100 publications

(50 citation statements)

References 17 publications

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“…There have been several studies that relate MDA and 4-hydroxynonenal levels with perinatal hypoxia, low birth weight and congenital malformations [25,26]. In our study all the newborns had good birth weight and adequate Apgar scores.…”

Section: Discussionmentioning

confidence: 58%

Malondialdehyde: A Possible Marker of Ageing

Gil¹,

Fariñas²,

Casado³

et al. 2002

Gerontology

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Background: Numerous recent studies have suggested that oxidative damage may be important in the ageing process, and lipid peroxidation is an important biological consequence of oxidative cellular damage. Objective: The aim of this work was to analyze the activities of the two protective enzymes, superoxide dismutase (SOD) and catalase (CAT), and the levels of malondialdehyde (MDA) to examine the relationship between the ageing process and defence antioxidant and lipid peroxidation. Method: SOD activity was measured in red blood cells using the Minami and Yoshikawa method; CAT activity was measured in hemolysates by the Aebi method, and MDA levels were measured in erythrocytes by high-performance liquid chromatography. Results: SOD activity shows statistically significant differences between newborns and the rest of the sample (ANOVA p < 0.001; Student-Newman-Keuls test p < 0.001). CAT activity did not show significant differences between the age groups. We observed statistically significant differences in MDA levels between the different groups (ANOVA p < 0.001; Student-Newman-Keuls test p < 0.05). In the regression analysis and rectilinear/curvilinear adjustment compared to age, SOD and CAT showed coefficients close to zero (SOD linear = 0.16; SOD exponential = 0.15; CAT linear = 0.056; CAT exponential = 0.068), indicating in that way their independence from age. Only MDA obtained a regression coefficient superior to 0.75 (p < 0.05). The best adjustment was reached through an exponential expression, giving the following parametric relation: MDA = 103.117e^0.0021.AGE. No statistically significant variation in SOD and CAT activity and MDA levels, related to sex could be demonstrated. Conclusions: Our data show that old age is associated with an increase in systemic oxidative stress.

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Section: Discussionmentioning

confidence: 58%

Malondialdehyde: A Possible Marker of Ageing

Gil¹,

Fariñas²,

Casado³

et al. 2002

Gerontology

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“…Hypoxia has been previously shown to increase lipid peroxidation in a variety of other biological systems. After hypoxia, levels of the lipid peroxidation indicator MDA increase in plasma and tissues of male albino rats (Kurhaliuk, 2001;Sarada et al, 2002), lung of adult human skiers (Gü zel et al, 2000), and plasma of human perinatal fetus (Schmidt et al, 1996) and newborn infants (Buonocore et al, 1998). Hypoxia also induces an increase in MDA in liver, brain, and heart of chicken embryo (Stock et al, 1990) and rat heart (Chen et al, 1987).…”

Section: Discussionmentioning

confidence: 99%

Inhibition of Inducible Nitric-Oxide Synthase Protects Human T Cells from Hypoxia-Induced Apoptosis

Kiang¹,

Krishnan²,

Lu³

et al. 2007

Mol Pharmacol

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Sodium cyanide-induced chemical hypoxia triggers a series of biochemical alterations leading to apoptosis in many cell types, including T cells. It is known that chemical hypoxia promotes inducible nitric-oxide synthase (iNOS) gene transcription by activating its transcription factors. To determine whether iNOS and NO production are responsible for chemical hypoxia-induced apoptosis, we exposed human Jurkat T cells to sodium cyanide in the presence or absence of iNOS inhibitors. We found that iNOS expression is necessary for hypoxia-induced lipid peroxidation and leukotriene B 4 generation. The inhibition of iNOS limited T-cell apoptosis by decreasing the activity of caspase-3 without affecting the expression of Fas/Apo-1/CD95 on the surface membrane of T cells. These data suggest iNOS-mediated NO produced endogenously in the T cell alters overall T-cell function and results in apoptosis. Proper control of iNOS expressed in the T cell may represent a useful approach to immunomodulation.

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“…Malondialdehyde (MDA) is a product of such ROS-induced damage and reflects the degree of lipid peroxidation. 4 It is well documented that the fetus experiences significant reductions in oxygen saturation and short periods of moderate hypoxia-ischemia during the process of birth. 5,6 What is unclear is whether this degree of intermittent physiologic fetal hypoxemiaischemia during normal delivery is associated with quantifiable increases in hypoxanthine, xanthine, uric acid and malondialdehyde.…”

Section: Introductionmentioning

confidence: 99%

Effect of mode of birth on purine and malondialdehyde in umbilical arterial plasma in normal term newborns

Calderon

Rawson

et al. 2008

J Perinatol

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Objective: To examine the effect of mode of birth on plasma purine and malondialdehyde levels in normal term infants.Study Design: Umbilical arterial cord blood was obtained immediately after birth from a convenience sample of 119 normal term newborns born by vaginal delivery, with or without oxytocin augmentation or by elective cesarean delivery. Plasma was analyzed for purine and/or malondialdehyde levels. Numeric data were analyzed utilizing independent samples t-test and ordinal data were analyzed using MannWhitney test. Correlation coefficients were obtained using Spearman's r.Result: Uric acid levels were significantly elevated (P<0.001) in neonates undergoing vaginal birth, compared to neonates born by elective cesarean delivery. When the effect of oxytocin and length of labor was analyzed, neonates born to mothers on oxytocin had lower hypoxanthine, significantly lower xanthine (P ¼ 0.05) and higher uric acid levels. In addition, malondialdehyde levels were significantly higher (P<0.006) in neonates born to mothers who received oxytocin compared to neonates born to mothers without oxytocin augmentation. We also found significant correlations between malondialdehyde (MDA) and hypoxanthine (r ¼ À0.465, P<0.039) and between MDA and xanthine (r ¼ À0.753, P ¼ 0.003) in neonates born via oxytocin-augmented birth. Mode of birth had no statistically significant effect on clinical outcomes, although infants born by elective cesarean had higher incidence of acute respiratory distress and transient tachypnea of the newborn compared to those born vaginally. Conclusion:Neonates born by elective cesarean had the lowest purine levels in cord blood compared to neonates born vaginally. Oxytocin augmentation is associated with some degree of uterine hyperstimulation which may enhance the ATP degradation pathway resulting in the rapid conversion of hypoxanthine to xanthine and xanthine to uric acid. Significantly higher MDA levels in neonates whose mothers received oxytocin as well as significant correlation between MDA and the purines hypoxanthine and xanthine, suggest free-radical production, most likely due to xanthine oxidase activation. However, despite differences in plasma purine and malondialdehyde levels, no significant differences were seen in neonatal outcome. Further studies are required to fully characterize the effect of mode of birth on purine metabolism and free-radical production.

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Increased Levels of Lipid Peroxidation Products Malondialdehyde and 4-Hydroxynonenal after Perinatal Hypoxia

Cited by 100 publications

References 17 publications

Malondialdehyde: A Possible Marker of Ageing

Malondialdehyde: A Possible Marker of Ageing

Inhibition of Inducible Nitric-Oxide Synthase Protects Human T Cells from Hypoxia-Induced Apoptosis

Effect of mode of birth on purine and malondialdehyde in umbilical arterial plasma in normal term newborns

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