Increased lipid production of the marine oleaginous microalgae Isochrysis zhangjiangensis (Chrysophyta) by nitrogen supplement

Feng, Dina; Chen, Zhaoan; Xue, Song; Zhang, Wei

doi:10.1016/j.biortech.2011.04.006

Cited by 188 publications

(85 citation statements)

References 33 publications

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“…However, the effect of nonconventional media prepared from CAGF on microalgae growth [2,18,19] and biochemical composition [20,21] were investigated.…”

Section: Discussionmentioning

confidence: 99%

Enhance Growth and Biochemical Composition of Nannochloropsis oceanica, Cultured under Nutrient Limitation, Using Commercial Agricultural Fertilizers

Ashour¹,

Kamel²

2017

J Marine Sci Res Dev

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Microalgae culture media should be economic, allow for high growth, satisfy the needs of microalgal cells and easy to prepare. In this study, we evaluate the effect of different media formula prepared from commercial agricultural fertilizers (CAGF), comparing to F/2 Guillard standard medium as a control medium, on growth (cell density, CD; dry weight, DW and specific growth rate, µ) and biochemical composition (lipid, protein, and carbohydrate) of Nannochloropsis oceanica. Comparing to N/P ratio (9.6) and actually quantity (12.36 g/l and 1.29 g/l, respectively) of F/2 standard medium, six N/P ratios (19.2, 9.6, 9.6, 4.8, 3.2 and 1.6) were prepared from Nitric Acid (N-Nt) or Ammonium Sulphate (N-Am), as a nitrogen source, with phosphoric acid (P), as a phosphorus source, for culturing media of N. oceanica. The results investigated that some CAGF media achieved significant (P ≤ 0.05) growth and biochemical composition higher than F/2. Comparing to lipid percentage (30.70 %) of F/2, the lipid percentage of N. oceanica cultured on different CAGF media were ranging from 18.40% to 46.12%, depending on nutrient limitation, nitrogen source, N/P ratios and actually atom concentrations. Finally, the use of CAGF constitutes a viable alternative of F/2 medium to reduce the production costs N. oceanica, the commonly used in marine hatcheries and also other biotechnological applications.

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“…However, the effect of nonconventional media prepared from CAGF on microalgae growth [2,18,19] and biochemical composition [20,21] were investigated.…”

Section: Discussionmentioning

confidence: 99%

Enhance Growth and Biochemical Composition of Nannochloropsis oceanica, Cultured under Nutrient Limitation, Using Commercial Agricultural Fertilizers

Ashour¹,

Kamel²

2017

J Marine Sci Res Dev

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“…As with other algae, including Isochrysis zhangjiangensis, Cyclotella cryptica [35], and Isochrysis galbana [36], the activity of acetyl-CoA carboxylase or other key enzymes of T. suecica and C. minutissima may be enhanced under non-limiting nitrogen conditions, resulting in an increase in lipid production.…”

Section: Effect Of Nitrate On Lipid Production By Microalgaementioning

confidence: 99%

“…The increase in lipid content which takes place with the increase in nitrate availability has been attributed to an increase in the activity of acetyl-CoA carboxylase (ACCase), causing an increase in lipid production [35,36]. As with other algae, including Isochrysis zhangjiangensis, Cyclotella cryptica [35], and Isochrysis galbana [36], the activity of acetyl-CoA carboxylase or other key enzymes of T. suecica and C. minutissima may be enhanced under non-limiting nitrogen conditions, resulting in an increase in lipid production.…”

Section: Effect Of Nitrate On Lipid Production By Microalgaementioning

confidence: 99%

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Effect of nitrate on lipid production by T. suecica, M. contortum, and C. minutissima

et al. 2013

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Abstract:Microalgae are an alternative and sustainable source of lipids that can be used as a feedstock for biodiesel production. Nitrate is a good nitrogen source for many microalgae and affects biomass and lipid yields of microalgae. In this study, the effect of nitrate on cell growth and lipid production and composition in Monoraphidium contortum, Tetraselmis suecica, and Chlorella minutissima was investigated. Nitrate affected the production of biomass and the production and composition of lipids of the three microalgae tested. Increasing the nitrate concentration in the culture medium resulted in increased biomass production and higher biomass productivity. Furthermore, increasing the nitrate concentration resulted in a reduction in lipid content and productivity in M. contortum; however, the opposite effect was observed in T. suecica and C. minutissima cultures. diesel infrastructure for storage and distribution. Biodiesel offers several advantages over petroleumbased diesel. For example, biodiesel is a renewable and biodegradable energy resource, it produces fewer toxic emissions (carbon monoxide, aromatic compounds, hydrocarbons, particulate matter, sulfur oxides, nitrogen oxides, and metals), it is less volatile and safer to transport, store and handle, and it increases efficiency, reduces wear, and extends engine life [2,3].Currently, biodiesel is produced from vegetable oils (edible or inedible), waste oils, and animal fats. However, this practice has raised serious concerns in the international community regarding the production, price, and availability of food. Other concerns include the deforestation of large areas of land that could be used to grow oleaginous vegetables, the huge amounts of water needed for irrigation, and the inability of these biodiesel sources to meet current and future fuel demands [4,5]. A sustainable biodiesel industry needs alternative raw materials that can be obtained easily from alternative renewable and biodegradable sources, allowing continuous operation and avoiding the limitations described [6].Currently, there is a consensus that microalgae are an alternative and sustainable source of lipids that can be used as a feedstock for biodiesel production. Microalgae are suitable for this purpose because they are photosynthetic microorganisms with a simple cellular structure and are easy to culture. Furthermore, microalgae grow in a variety of environments, their growth rate is 20 to 30 times faster than other sources of biofuels, they exhibit high photosynthetic efficiency, contribute greatly to the sequestration of atmospheric CO 2 , thereby mitigating climate change, can be harvested 365 days a year, can be grown in areas unsuitable for agriculture, livestock, industry, or tourism, require smaller volumes of water than oleaginous plants and can use water unsuitable for human consumption, their intracellular lipid content is high and the productivity of lipids per unit area is considerably higher than that of oleaginous plants [7][8][9][10].Several studies have shown that...

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“…It must be noted that this study was carried out in a CO2-limited environment which could have led to reduced biomass productivity. However, there is evidence of an inverse relationship in biomass and lipid productivity for microalgae [39]. An assessment of lipid production as a dry weight percentage of the whole biomass by the diatoms Navicula pelliculosa, Navicula saprophila and Phaeodactylum tricornutum [37] showed that they all produced 28%, 26% and 22% lipid dwt, respectively, which compares well with the microalgal strains in this study whose lipid production by dry weight ranged from 8.9% ± 1.2% to 42.1% ± 2.6 %.…”

Section: Biomass and Lipid Content Of Screened Microalgae Strainsmentioning

confidence: 99%

Screening and Evaluation of Some Green Algal Strains (Chlorophyceae) Isolated from Freshwater and Soda Lakes for Biofuel Production

et al. 2015

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Microalgae are photosynthetic microorganisms that can produce lipids, proteins and carbohydrates in large amounts and within short periods of time and these can be processed into both biofuels and other useful commercial products. Due to this reason microalgae are considered as a potential source of renewable energy; and one of the most important decisions in obtaining oil from microalgae is the choice of species. In this study, the potential of Chlorophyceae species isolated from freshwater and soda lakes in Hungary and Romania (Central Europe) were characterized and evaluated by determining their biomass accumulation, lipid productivity, fatty acid profiles, and biodiesel properties besides protein and carbohydrate productivity. Out of nine strains tested, three accumulated more than 40% dry weight of protein, four accumulated more than 30% dry weight of carbohydrate and the strain Chlorella vulgaris LC8 accumulated high lipid content (42.1% ± 2.6%) with a favorable C16-C18 fatty acid profile (77.4%) as well as suitable biodiesel properties of high cetane number (57.3), low viscosity (4.7 mm 2 /s), lower iodine number (75.18 g I2/100 g), relative cloud point (8.8 °C) and negative cold filter plugging point (−6.5 °C). Hence the OPEN ACCESSEnergies 2015, 8 7503 new strain, Chlorella vulgaris LC8 has potential as a feedstock for the production of excellent quality biodiesel.

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Increased lipid production of the marine oleaginous microalgae Isochrysis zhangjiangensis (Chrysophyta) by nitrogen supplement

Cited by 188 publications

References 33 publications

Enhance Growth and Biochemical Composition of Nannochloropsis oceanica, Cultured under Nutrient Limitation, Using Commercial Agricultural Fertilizers

Enhance Growth and Biochemical Composition of Nannochloropsis oceanica, Cultured under Nutrient Limitation, Using Commercial Agricultural Fertilizers

Effect of nitrate on lipid production by T. suecica, M. contortum, and C. minutissima

Screening and Evaluation of Some Green Algal Strains (Chlorophyceae) Isolated from Freshwater and Soda Lakes for Biofuel Production

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